A5008923840- Metalloenzymes and iron-sulfur proteins
- Electrocatalysts for Energy Conversion
- Intracranial Aneurysms: Treatment and Complications
- Advanced battery technologies research
- Blood properties and coagulation
- Electrochemical sensors and biosensors
- Cerebrovascular and Carotid Artery Diseases
- Neonatal Respiratory Health Research
- Erythrocyte Function and Pathophysiology
- Iron Metabolism and Disorders
- Glycosylation and Glycoproteins Research
- Hydrogen Storage and Materials
- Microbial Fuel Cells and Bioremediation
- Infant Nutrition and Health
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Traumatic Brain Injury and Neurovascular Disturbances
- S100 Proteins and Annexins
- Pregnancy and preeclampsia studies
- Endometriosis Research and Treatment
- Photosynthetic Processes and Mechanisms
- Biomarkers in Disease Mechanisms
- Neurological Disorders and Treatments
- Anaerobic Digestion and Biogas Production
- Spinal Fractures and Fixation Techniques
Institute of Basic Biological Problems
2010-2023
Novokuznetsk State Institute of Advanced Medical Education
1994-2018
Dnipropetrovsk State Medical Academy
2018
Russian Academy of Sciences
1996-2015
Clinical Hospital No. 8
2011
Montana State University
2005-2009
Krasnoyarsk Regional Clinical Hospital
2009
Fudan University
2008
Hamilton College
2005
University of Massachusetts Amherst
2005
Enzymes power up: The limiting performance characteristics of hydrogenases in bioelectrocatalysis was studied. A comparison with catalysis by noble metals such as Pt, used low-temperature H2–O2 fuel cells, shows that hydrogenase electrodes are similarly active the consumption dissolved H2 Pt-based electrodes.
The H-D exchange reaction has been measured with the D2-H2O system, for Rhodobacter capsulatus JP91, which lacks hupSL-encoded hydrogenase, and R. BSE16, HupUV proteins. hupUV gene products, expressed from plasmid pAC206, are shown to catalyze an distinguishable due membrane-bound, hydrogenase. In presence of O2, uptake hydrogenase BSE16 cells catalyzed a rapid oxidation H2, D2, HD present in its activity (H-D exchange, H2 evolution reduced methyl viologen [MV+]) depended on external pH,...
Considering crucial problems that limit use of platinum-based fuel cells, i.e. cost and availability, poisoning by impurities low selectivity, we propose electrocatalysis enzymes as a valuable alternative to noble metals. Hydrogenase electrodes in neutral media achieve hydrogen equilibrium potential (providing 100% energy conversion), display high activity H2 electrooxidation, which is similar Pt-based sulphuric acid. In contrast with platinum, enzyme are highly selective for their...
The kinetics of H‐D isotope exchange catalyzed by the thermostable hydrogenase from Thiocapsa roseopersicina have been studied analysis between D 1 and H 2 O. pH dependence reaction was examined 2.5 11. Over whole range, HD produced at a higher initial velocity than , with marked optimum 5.5; second peak in profile observed around 8.5. rapid formation respect to /H O system is consistent heterolytic cleavage into + an enzyme hydride that can both solvent. H‐D‐exchange activity lower /D...
Pyridylthio-modified multiwalled carbon nanotubes (pythio-MWNTs) have been prepared by a reaction of the oxidized MWNTs with S-(2-aminoethylthio)-2-thiopyridine hydrochloride. The obtained pythio-MWNTs nanocomposites formed stable floating monolayers at air−water interface, which were transferred onto substrate surfaces Langmuir−Blodgett (LB) method. Compositions and morphologies LB films characterized absorption, Raman, X-ray photoelectron spectra as well scan electron microscopy atomic...
ABSTRACT Rhodobacter capsulatus synthesizes two homologous protein complexes capable of activating molecular H 2 , a membrane-bound [NiFe] hydrogenase (HupSL) linked to the respiratory chain, and an sensor encoded by hupUV genes. The activities hydrogen-deuterium (H-D) exchange catalyzed hupSL -encoded enzymes in presence D O were studied comparatively. Whereas HupSL is membranes, HupUV activity was localized soluble cytoplasmic fraction. Since gene cluster R. contains hoxH which encodes...
The catalytic and spectroscopic properties of the reversible hydrogenase from cyanobacterium Anabaena variabilis have been examined. required reductive activation in order to elicit hydrogen‐oxidation activity. Carbon monoxide was a weak ( K i = 35 μ M), competitive inhibitor. A flavin with chromatographic FMN, nickel were detected purified enzyme. A. exhibited electron paramagnetic resonance (EPR) spectra its hydrogen‐reduced state, indicative [2Fe‐2S] [4Fe‐4S] clusters. Although no EPR...
Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis, is required by all three domains life. Here, we report purification biochemical structural characterization enolase from Chloroflexus aurantiacus, a thermophilic anoxygenic phototroph affiliated with green non-sulfur bacteria. The protein was purified as homodimer subunit molecular weight 46 kDa. temperature optimum for catalysis 80°C, close measured thermal stability...
Hydrogenases encapulated in porous polymeric silica gels retain significant levels of hydrogen production activity when compared to hydrogenases solution using reduced methyl viologen as an electron donor. Encapsulated remain active after storage at room temperature for longer than four weeks and are less sensitive proteolytic digestion. Nanoscopic confinement solids paves the way their potential use producing catalytic materials applications.
An der Grenze: Die Grenzleistung von Hydrogenasen in Bioelektrokatalyse wurde untersucht. Ein Vergleich mit Katalyse durch Edelmetalle wie Platin, das H2/O2-Niedertemperatur-Brennstoffzellen zum Einsatz kommt, zeigt, dass Hydrogenase-Elektroden ähnlich aktiv beim Verbrauch gelöstem H2 sind Pt-Elektroden. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2007/z701096_s.pdf or from author. Please note: The publisher not responsible...