A5105409989- Retinal Development and Disorders
- Lipid Membrane Structure and Behavior
- Influenza Virus Research Studies
- Photoreceptor and optogenetics research
- Retinal and Optic Conditions
- Neuroscience and Neuropharmacology Research
- Ocular and Laser Science Research
- Respiratory viral infections research
- Glaucoma and retinal disorders
- ZnO doping and properties
- Solidification and crystal growth phenomena
- RNA and protein synthesis mechanisms
- Retinal Imaging and Analysis
- Heat Transfer and Boiling Studies
- Erythrocyte Function and Pathophysiology
- Spacecraft and Cryogenic Technologies
- Intraocular Surgery and Lenses
- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Ocular Infections and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Cellular transport and secretion
- Enzyme Catalysis and Immobilization
- Crystallization and Solubility Studies
- melanin and skin pigmentation
National Institute of Advanced Industrial Science and Technology
2011-2023
National Institute of Technology
2018
Nihon University
2018
Sendai National College of Technology
2018
Midorigaoka Hospital
2016-2017
Kyiv City Clinical Oncology Center
2013
Kaw Nation
2012
Biomedical Research Institute
2010
Shibaura Institute of Technology
2008
National Institute of Infectious Diseases
2007
Aptamers selected against various kinds of targets have shown remarkable specificity and affinity, similar to those displayed by antibodies their antigens. To employ aptamers as genotyping reagents for the identification pathogens strains, in vitro selections were carried out find that specifically bind distinguish closely related human influenza A virus subtype H3N2. The aptamer, P30-10-16, binds haemagglutinin (HA) region target strain A/Panama/2007/1999(H3N2) failed recognize other...
The molecular mechanism of hemolysis and fusion by influenza virus in acidic media was studied. First, the effect trypsin treatment on activity fibroblast-grown results showed that split form viral hemagglutinin, HA1 HA2, but not precursor, is responsible for activity. Second, interaction egg-grown virus, which contains with lipid liposomes studied spin labeling electron microscopy. Phospholipid transfer from envelope to bilayer membrane occurred within 30 s at pH 4.5-5.4. largely...
Interaction between influenza virus WSN strain and MDCK cells was studied by using spin-labeled phospholipids electron microscopy. Envelope fusion negligibly small at neutral pH but greatly activated in acidic media a narrow range around 5.0. The half-time less than 1 min 37 degrees C Virus binding almost independent of the pH. Endocytosis occurred with about 7 pH, 50% initially bound internalized after h. Electron micrographs showed particles coated pits microvillous surface plasma membrane...
Hemagglutinin (HA) glycoproteins isolated from influenza virus caused hemolysis and liposome lysis at pH less than 6.0. The dependence was similar to that of the parent virus. Hemagglutination titers HA were comparable with those time course by somewhat different did not cause fusion erythrocytes in acidic media, contrast Both virus, previously incubated 6.0, lost their low-pH-induced hemolytic activity. Isolated formed rosette-like structures neutral pH, these aggregated media. Virus also...
Rotational diffusion of erythrocyte anion channel protein band 3 was measured in ghost membranes by observing time-resolved phosphorescence anisotropy decays eosinyl-5-maleimide covalently attached to the protein. Experiments were carried out under conditions similar those employed Tsuji and Ohnishi (1986) for translational measurement [(1986) Biochemistry 25, 6133-6139] allow direct comparison rotational 3. Detailed analysis diffusive properties made on basis these data. measurements...
Aptamers are known for their higher discriminating ability between closely related molecules and requirement only a small region binding, as compared to an antibody. In the present studies, we have isolated specific RNA aptamer against influenza virus B/Johannesburg/05/1999 by in vitro selection procedure. The bound efficiently HA of B required 5 mM MgCl2 ion its recognition. not distinguished derived from A virus, but also inhibited HA-mediated membrane fusion.
The structure of membrane fusion intermediates between the A/PR/8(H1N1) strain influenza virus and a liposome composed egg phosphatidylcholine, cholesterol, glycophorin was studied using quick-freezing electron microscopy. Fusion by viral hemagglutinin protein induced at pH 5.0 23°C. After 19-s incubation under these conditions, small protrusions with diameter 10–20 nm were found on fractured convex faces liposomal membranes, pits complementary to concave faces. corresponded parts outward...
1. To study the effects of brief periods hypoxia on cellular functions in rat hippocampal slice, extracellular and intracellular recordings were made from pyramidal neurons, interstitial potassium activity ([K+]o) was measured cell layers. Slices perfused an interface chamber at 36-37 degrees C with medium containing 8.5 mM [K+]o. Hypoxia induced by switching overflow gas O2-CO2 to N2-CO2. 2. Brief (5-60 s) produced electrographic seizures typical tonic clonic components 53% 293 slices that...
Gaucher disease (GD) is the most common of lysosomal storage disorders and caused by defects in GBA gene encoding glucocerebrosidase (GlcCerase). The accumulation its substrate, glucocylceramide (GlcCer) considered main cause GD. We found here that expression human mutated GlcCerase (hGBA) associated with neuronopathy GD patients causes neurodevelopmental Drosophila eyes. data indicate endoplasmic reticulum (ER) stress was elevated eye carrying hGBAs using ER markers dXBP1 dBiP. also...
Exosomes (diameter 30-200 nm) are a subtype of extracellular vesicles secreted by cells containing DNA, microRNA (miRNA), and proteins. expected to be valuable as means delivering drugs or functional miRNAs in treatment diseases. However, the delivery exosomes is not sufficiently effective, even though have intrinsic functions. Cell-penetrating peptides (CPPs) short peptide families that facilitate cellular intake molecules vesicles. We previously reported modification cells, liposomes with...
ABSTRACT Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n -alkane under nitrogen-limiting conditions. Under the optimized conditions with -hexadecane as substrate, hexadecyl hexadecanoate in cells reached 0.17 g/g (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant ester formation. The contribution acyl-CoA reductase to synthesis was evaluated by gene disruption analysis.
Influenza virus hemagglutinin (HA) is a determinant of infectivity. Therefore, it important to determine whether HA new influenza virus, which can potentially cause pandemics, functional against human cells. The novel imaging technique reported here allows rapid analysis function by visualizing viral fusion inside This was designed detect changing the spectrum fluorescence-labeled virus. Using this imaging, we detected between and very small endosome that could not be previously, indicating...
Sulfatide is abundantly expressed in various mammalian organs, including the intestines and trachea, which influenza A viruses (IAVs) replicate. However, function of sulfatide IAV infection remains unknown. synthesized by two transferases, ceramide galactosyltransferase (CGT) cerebroside sulfotransferase (CST), degraded arylsulfatase (ASA). In this study, we demonstrated that enhanced replication through efficient translocation newly nucleoprotein (NP) from nucleus to cytoplasm, using...
In the direct melt bonding of isotactic polypropylene (iPP) to aluminum (Al), blending a small amount maleic anhydride-grafted PP (PPgMA) with iPP was found induce dramatic improvement strength adhesion. The effect PPgMA was, however, limited, maximizing at ∼20 wt % PPgMA. Incorporation larger amounts reduced We studied mechanism adhesion between Al and by incorporating chemical functionality polymer side. fracture surfaces produced peeling off interfaces were investigated replicating...
A method including surface silanization, phase transfer and self-assembly, SiO2 shell growth has been developed to incorporate multiple hydrophobic CdSe/ZnS nanocrystals into beads where they are well suited for bio-application due their high brightness, less-cytotoxic, non-blinking nature.
Plant cellulose is the most abundant organic compound on earth. Technologies for producing fiber or improving enzymatic saccharification of hold key to biomass applications. A technology atomizing without strong acid catalysis remains be developed. The water jet a well-known device used in machines (e.g., washing machines, cutters, and mills) that use high-pressure water. In this study, we examined whether system could atomize crystalline cellulose, which comprises approximately 50% plant...