Nitric oxide (NO) functions as a signaling molecule in plant adaptation to changing environmental conditions. NO levels were found increase plants response low temperatures (LTs). However, knowledge of the pathways involved enhanced production under cold stress is still limited. For this reason, we aimed determine role different sources generation cucumber roots exposed 10 °C for short (1 d) and long (6 periods. The short-term treatment seedlings with LT markedly increased plasma...

Vacuolar H+ -ATPase (V-ATPase; EC 3.6.3.14) is the main enzyme responsible for generating a proton gradient across tonoplast. Under cadmium (Cd) stress conditions, V-ATPase activity inhibited. In present work, hydrogen sulfide (H2 S) and peroxide O2 ) cross-talk was analyzed in cucumber (Cucumis sativus L.) seedlings exposed to Cd explain role of both signaling molecules control V-ATPase. gene expression as well H2 S content endogenous production were determined roots plants treated with 100...

In the present study we demonstrate that signalling molecules NO, H2O2 and H2S are important for understanding mechanisms of modification plasma membrane H+-ATPase (EC 3.6.3.14) activity in conditions both salt (50mM NaCl) low temperature (10°C, LT) stress. Plants were subjected to stress 1 or 6 days. After 3 days exposure some plants transferred control another days: post-stressed (3+3). We measured endogenous levels stressed plants. To determine physiological significance induced (PM...

Water and nutrient deficiencies in soil are becoming a serious threat to crop production. Therefore, usable water recovery from wastewater, such as urine grey water, should be considered. In this work, we showed the possibility of using after processing an aerobic reactor with activated sludge which nitrification process takes place. The resulting liquid (nitrified NUG) contains three potential factors that can adversely affect plant growth hydroponic system: anionic surfactants, deficits,...

Plasma membrane NADPH oxidases (RBOHs, EC 1.6.3.1) are known as the main ROS generators involved in plant adaptation to stress conditions. In present work, regulation of oxidase was analyzed cucumber (Cucumis sativus L. var. Krak) seedlings exposed salinity. RBOH activity and gene expression, well H2O2 content, were determined roots plants treated with 50 or 100 mM NaCl for 1 h, 6 days. It found that enzyme increased parallel an enhancement level day. The expression some CsRboh genes induced...

Two electrogenic proton pumps, vacuolar H(+) transporting ATPase (V-ATPase, EC 3.6.3.14) and inorganic pyrophosphatase (V-PPase, 3.6.1.1), co-exist in the membrane of plant cells. In this work, all CsVHA CsVHP genes encoding V-ATPase V-PPase, respectively, were identified cucumber genome. Among them, three CsVHA-c for subunit c two CsVHP1 type I V-PPase analyzed detail. Individual isogenes differentially regulated tissues during development as well under changing environmental conditions....

When plants are exposed to environmental stress, their growth is inhibited. Under such conditions, controlled inhibition of beneficial for plant survival. Jasmonic acid (JA) a well-known phytohormone that limits growth, which has been confirmed in several species. However, its role cucumber seedlings not yet comprehensively investigated. For this reason, we aimed determine the involvement JA regulation proteins crucial including plasma membrane proton pump (PM H+-ATPase), PM nitrate...

Polyamines (PAs) are small aliphatic compounds that participate in the plant response to abiotic stresses. They also nitric oxide (NO) production plants; however, their role this process remains unknown. Therefore, study aimed investigate of putrescine (Put) NO roots cucumber seedlings subjected salt stress (120 mM NaCl) for 1 and 24 h. In salinity, exogenous Put can regulate levels by managing biosynthesis pathways a time-dependent manner. exposed h reduced level decreasing nitrate...

As in plants sugar sensing and signal transduction involve pathways dependent or independent on hexokinase 1 ( HXK1 ) as a glucose sensor, research was conducted to determine which pathway is responsible for regulation of the nitrate reduction. An Arabidopsis mutant with T‐ DNA insertion AtHXK1 gene defects signaling hxk1 used reductase NR activity, NIA genes expression leaves after 8‐h treatment sugars (glucose sucrose), organic acids [2‐oxoglutarate (2OG)] amino (glutamine glutamate)....

Cucumber (Cucumis sativus L.) is a crop plant being the third most-produced vegetable developed as new model plant. Heavy metal pollution serious global problem that affects production. An industrial activity has led to high emissions of Cd into environment. Plants realize adaptive strategies diminish toxic effects Cd. They can remove excess ions heavy metals from cytoplasm outside cells using metal/proton antiport. The proton gradient needed for action antiporter generated by plasma...

The regulatory properties of NADH-dependent nitrate reductase (NR) in desalted root extracts from hydroponically grown cucumber (Cucumis sativus L.) seedlings were examined. lowest activity NR was detected incubated with Mg2+ and ATP. An inhibitory effect Mg-ATP cancelled the presence staurosporine (the protein kinase inhibitor) completely reversed after addition ethylenediaminetetraacetate (EDTA) as well AMP into reaction mixture. Reactivation enzyme due to presence, contrary...

The effect of different oxygen availability on the nitrate reductase (NR, EC 1.6.6.1) activity in cucumber roots was studied. NR measured presence Mg2+ (actual activity) as well with EDTA (maximum increased distinctly after 30 min root incubation a medium flushed N2 (anaerobic conditions). In contrast, aeration (aerobic conditions) decreased both enzyme activities. Such inactivation rapidly reversed transferring to anaerobic conditions. An air-induced decrease actual prevented by...