A5063998638- Extraction and Separation Processes
- Geochemistry and Elemental Analysis
- Methane Hydrates and Related Phenomena
- Biofuel production and bioconversion
- Hydrocarbon exploration and reservoir analysis
- Microbial Metabolic Engineering and Bioproduction
- Metal Extraction and Bioleaching
- Recycling and Waste Management Techniques
- Microbial metabolism and enzyme function
- Spaceflight effects on biology
- Atmospheric and Environmental Gas Dynamics
- Radioactive element chemistry and processing
- Geological and Geophysical Studies
- Enzyme Production and Characterization
- Microbial Fuel Cells and Bioremediation
- Muscle Physiology and Disorders
- Anaerobic Digestion and Biogas Production
- Biochemical Acid Research Studies
- Light effects on plants
- Amino Acid Enzymes and Metabolism
- Planetary Science and Exploration
- Inflammatory mediators and NSAID effects
- Exercise and Physiological Responses
- Microbial Community Ecology and Physiology
- Space Science and Extraterrestrial Life
Idaho National Laboratory
2013-2024
Kennedy Space Center
2005-2022
Lurie Children's Hospital
2015-2021
Northwestern University
2018
University of Idaho
1998-2010
Ohio University
1997-2009
Pennsylvania State University
1997-2007
University of Connecticut
2007
Bionetics (United States)
2005
University of Florida
2005
ABSTRACT Microbial communities in cores obtained from methane hydrate-bearing deep marine sediments (down to more than 300 m below the seafloor) forearc basin of Nankai Trough near Japan were characterized with cultivation-dependent and -independent techniques. Acridine orange direct count data indicated that cell numbers generally decreased sediment depth. Lipid biomarker analyses presence viable biomass at concentrations greater previously reported for terrestrial subsurface environments...
Programmed cell death (apoptosis) is an intrinsic part of organismal development and aging. Here we report that many nonsteroidal antiinflammatory drugs (NSAIDs) cause apoptosis when applied to v-src-transformed chicken embryo fibroblasts (CEFs). Cell was characterized by morphological changes, the induction tissue transglutaminase, autodigestion DNA. Dexamethasone, a repressor cyclooxygenase (COX) 2, neither induced nor altered NSAID effect. Prostaglandin E2, primary eicosanoid made CEFs,...
With the increasing demand for rare earth elements (REEs) in many emerging clean energy technologies, there is an urgent need development of new approaches efficient REE extraction and recovery. As a step toward this goal, we genetically engineered aerobic bacterium Caulobacter crescentus adsorption through high-density cell surface display lanthanide binding tags (LBTs) on its S-layer. The LBT-displayed strains exhibited enhanced REEs compared to cells lacking LBT, high specificity REEs,...
This study was a preliminary evaluation of ureolytically driven calcite precipitation and strontium coprecipitation for remediating 90Sr contamination at the Hanford 100-N Area in Washington; particular approach is suitable treating sorbed that could otherwise be long-term source groundwater contamination. Geochemical conditions site are compatible with stability, therefore sediment samples were examined to assess ureolytic capabilities native microbiota. Quantitative assays detected up 2 ×...
A bioleaching process to extract rare-earth elements (REE) from fluidized catalytic cracking (FCC) catalysts was optimized using a heterotrophic bacterium Gluconobacter oxydans produce organic acids glucose. Parameters included agitation intensity, oxygen levels, glucose concentrations, and nutrient additions. Biolixiviants the batch demonstrated REE leaching efficiencies up 56%. continuous bioreactor system subsequently developed feed leach of 51%. techno-economic analysis showed be single...
Lanmodulin (LanM) is a recently discovered protein that undergoes large conformational change in response to rare-earth elements (REEs). Here, we use multiple physicochemical methods demonstrate LanM the most selective macromolecule for REEs characterized date and even outperforms many synthetic chelators. Moreover, exhibits metal-binding properties structural stability unseen other metalloproteins. retains REE binding down pH ≈ 2.5, LanM-REE complexes withstand high temperature (up 95 °C),...
The use of biomass for adsorption rare earth elements (REEs) has been the subject many recent investigations. However, REE by bioengineered systems scarcely documented, and rarely tested with complex natural feedstocks. Herein, we engineered E. coli cells enhanced cell surface-mediated extraction REEs functionalizing OmpA protein 16 copies a lanthanide binding tag (LBT). Through biosorption experiments conducted leachates from metal-mine tailings deposits, show that functionalization surface...
Methane hydrate found in marine sediments is thought to contain gigaton quantities of methane and considered an important potential fuel source climate-forcing agent. Much the hydrates biogenic, so models that predict presence distribution require accurate rates situ methanogenesis. We estimated methanogenesis Hydrate Ridge (HR) by coupling experimentally derived minimal methanogen biomass determinations for discrete locations sediment column. When starved a recycle reactor, Methanoculleus...
Rare earth elements (REEs) are indispensable components of many green technologies and increasing demand globally. However, refining REEs from raw materials using current is energy intensive enviromentally damaging. Here, we describe the development a novel biosorption-based flow-through process for selective REE recovery electronic wastes. An Escherichia coli strain previously engineered to display lanthanide-binding tags on cell surface was encapsulated within permeable polyethylene glycol...
Agricultural waste was used as substrate for fermentation by Gluconobacter oxydans to produce lixiviant rare earth element (REE) recovery from industrial materials, i.e., spent fluid catalytic cracking (FCC) catalysts. Biolixiviant generated potato wastes performed comparably that refined glucose (25.7% ±0.2 and 25.1% ±1.1 REE recovered, respectively). Corn stover yielded a slightly less effective biolixiviant (23.3% ±0.3 recovered) but may serve better since collection systems are already...
The current uncertainty in the global supply of rare earth elements (REEs) necessitates development novel extraction technologies that utilize a variety REE source materials. Herein, we examined techno-economic performance integrating biosorption approach into large-scale process for producing salable total oxides (TREOs) from various feedstocks. An airlift bioreactor is proposed to carry out mediated by bioengineered earth-adsorbing bacteria. Techno-economic assessments were compared three...
The nematode Caenorhabditis elegans, a popular organism for biological studies, is being developed as model system space biology. chemically defined liquid medium, C. elegans Maintenance Medium (CeMM), allows axenic cultivation and automation of experiments that are critical spaceflight research. To validate CeMM use during spaceflight, we grew animals using standard laboratory conditions onboard STS-107, shuttle Columbia. Tragically, the Columbia was destroyed while reentering Earth's...
ABSTRACT To evaluate the potential for organic nitrogen addition to stimulate in situ growth of ammonia oxidizers during a field scale bioremediation trial, samples collected from Eastern Snake River Plain Aquifer Idaho before, during, and after molasses urea were subjected PCR analysis monooxygenase subunit A ( amoA ) genes. Ammonia-oxidizing bacteria (AOB) archaea (AOA) present all tested, with AOA genes outnumbering AOB samples. Following addition, nitrate levels rose bacterial copy...
Wet anaerobic storage of corn stover can provide a year-round supply feedstock to biorefineries meanwhile serving an active management approach reduce the risks associated with fire loss and microbial degradation. logistics systems employ particle size reduction early in chain through field-chopping which removes dependency on drying prior baling, expands harvest window, diminishes biorefinery requirements. Over two years, in-field forage chopping was capable reducing over 60% 6 mm or less....
Methanotrophic bacteria play an important role in global cycling of carbon and co-metabolism contaminants. Methanotrophs from pristine regions the Snake River Plain Aquifer (SRPA; Idaho, USA) were studied order to gain insight into native groundwater communities' genetic potential carry out TCE co-metabolism. Wells selected that proximal a plume believed be undergoing natural attenuation. Methane concentrations ranged 1 >1000 nM. Carbon isotope ratios diversity data together suggest SRPA...
Organic acid-based bioleaching has attracted significant research interest for the recovery of rare earth elements (REEs) and other critical metals. Utilizing biologically produced leaching agents, known as biolixiviants, derived from waste materials holds great promise enhancing economic viability environmental sustainability processes. This study focuses on modeling optimization biolixiviant production using corn stover (CS), date palm clippings (DP), nonrecyclable paper (NP)....