Sperm is particularly susceptible to reactive oxygen species (ROS) during critical phases of spermiogenesis. However, the level seminal ROS restricted by antioxidants which have beneficial effects on sperm parameters and developmental potentials. Mitochondria plasma membrane are two major sites generation in cells. Besides, leukocytes including polymer phonuclear (PMN) macrophages produce broad category molecules free radicals, non-radical nitrogen species. Physiological role increase...

Varicocele occurs in approximately 15% to 20% of the general male population and it is most common cause poor semen production decreased quality. It has been demonstrated that patients with varicocele have a significantly higher DNA fragmentation index (DFI) spermatozoa nuclear anomalies than healthy fertile men. Therefore, aim this study was evaluate sperm chromatin integrity these patients. Sixty men referring andrology laboratory were categorised into three different groups: 20 infertile...

The particles in the range of 1-100 nm are called nanoparticles. Gold nanoparticle is one most important metal nanoparticles with wide usage.

To investigate sperm chromatin/DNA integrity, global DNA methylation, and DNMT mRNA transcription in men with oligoasthenoteratozoospermia (OAT) compared normozoospermic men.Semen samples from 32 OAT patients who comprised the case group control were isolated purified using a standard gradient isolation procedure according to World Health Organization criteria. DNMT1, DNMT3A, DNMT3B transcripts then between groups real-time quantitative reverse-transcription polymerase chain reaction. Global...

In recent years, smoking water pipes or hookah has increased among adolescents in most countries. Although there is evidence support of the negative effects this type on human health, such as risk lung disease, little known about potential male reproductive system, especially molecular aspects sperm.This cross-sectional study examined sperm DNA fragmentation index, protamine 1 and 2 (PRM1 PRM2) genes expression, oxidant status normozoospermic smokers comparison with non-smoker controls.Our...

ObjectivesTo evaluate the effect of cord injury on (1) sperm parameters and (2) DNA chromatin status.DesignCase–control study.SettingData were collected from men referred to Research Clinical Center for Infertility, Yazd, Iran.ParticipantsThirty infertile with presence any level spinal (SCI) compared 30 healthy donors definite fertility normal parameters.InterventionsNot applicable.Outcome measuresSperm integrity was assessed using aniline blue (AB), chromomycin A3 (CMA3), toluidine (TB),...

The aim of this study was to survey the impact alcohol consumption on sperm parameters and DNA integrity in experimentally induced diabetic mice. A total 32 adult male mice were divided into four groups: group 1 served as control fed basal diet, 2 received streptozotocin (STZ) (200 mg kg(-1) , single dose, intraperitoneal) 3 (10 water soluble) 4 STZ for 35 days. cauda epididymidis each mouse dissected placed ml pre-warm Ham's F10 culture medium 30 min. swim-out spermatozoa analysed count,...

To evaluate the effects of zinc oxide nanoparticles (ZNPs) on morphometric and stereological parameters mouse testis.ZNPs are increasingly used in sunscreens, biosensors, food additives, pigments, rubber manufacture, electronic materials. However, potential toxicity these is not well understood.Experimental Groups (ZNP-1, ZNP-2 ZNP-3) received one following treatments daily for 35 days: 5, 50 300 mg/kg ZNPs respectively. Right testis from each animal was fixed bouin's solution measurement...

Globozoospermia is a severe form of teratozoospermia with low incidence in infertile patients, considered as one the important causes male infertility. The objective was to investigate chromatin/DNA integrity well apoptosis ejaculated spermatozoa cases partial or total globozoospermia. Fifty-seven semen samples were divided into three groups globozoospermia (n = 17), 10) and normozoospermia (control; n 30). Sperm chromatin condensation, DNA assessed using cytochemical assays. results showed...

The chief outcome of testicular torsion in clinical and experimental contexts is ischemia. Curcumin, a compound with anti-inflammatory antioxidant properties, has fascinated researchers clinicians for its promise the treatment fertility diseases.Thirty-five fully grown male mice were randomly classified into five groups: control, sham, torsion, group 1 (testicular torsion+short-term curcumin), 2 torsion+long-term curcumin). Thirty-five days later, spermatozoa from right cauda epididymis...

Previous studies have given conflicting results about the effect of generally infection and Chlamydia trachomatis on seminal ILs semen parameters. The aim this study was to investigate relationship between quality level interleukins (ILs) in infertile couples with C. trachomatis. Blood, first void urine (FVU) were obtained from 250 men who had failed conceive after 12 months trying. Serological analysis for specific IgA, IgM IgG antibodies serum, presence FVU sample carried out. main are as...

Our objective was to evaluate the effect of IMSI on embryo kinetics and clinical outcomes in patients with different aetiologies male infertility. A total 150 couples infertility were randomly divided into ICSI treatment groups (n = 75). done accordingly. For group, sperm selection using MSOME criteria then injected. The zygotes cultured time-lapse monitoring system (TLM) for 3 days. 650 embryos developed assessed TLM two groups. Data showed rate fragmentation had significant correlation (p...

Background: Generation of free radicals and oxidative stress are a major contributorto diabetes. These factors lead to the development diabetic testicles disorders.Objective: In this study, protective effect vitamin E on functional disordersassociated with diabetes induced in male reproductive systems hasbeen investigated.Materials Methods: Thirty-three adult Mice were divided into control,diabetic, untreated groups. Streptozotocin was used induce diabetes.In treated group, given...