A5044407583- Tendon Structure and Treatment
- Shoulder Injury and Treatment
- Sports injuries and prevention
- Exercise and Physiological Responses
- Orthopedic Surgery and Rehabilitation
- Muscle Physiology and Disorders
- Knee injuries and reconstruction techniques
- Muscle metabolism and nutrition
- Osteoarthritis Treatment and Mechanisms
- Laser Applications in Dentistry and Medicine
- Myofascial pain diagnosis and treatment
- Bee Products Chemical Analysis
- Connective tissue disorders research
- Cellular Mechanics and Interactions
- Sports Performance and Training
- Protease and Inhibitor Mechanisms
- Cardiovascular Effects of Exercise
- Dermatological and Skeletal Disorders
- Connective Tissue Growth Factor Research
- Muscle activation and electromyography studies
- RNA Research and Splicing
- Growth Hormone and Insulin-like Growth Factors
- Nutrition and Health in Aging
- Veterinary Equine Medical Research
- Lower Extremity Biomechanics and Pathologies
University of Copenhagen
2010-2022
Copenhagen University Hospital
2008-2022
Frederiksberg Hospital
2010-2020
Bispebjerg Hospital
2009-2019
University of Southern Denmark
2011
Odense University Hospital
2011
Capital Region of Denmark
2010
Gigtforeningen
2005
Tendons are often injured and heal poorly. Whether this is caused by a slow tissue turnover unknown, since existing data provide diverging estimates of tendon protein half-life that range from 2 mo to 200 yr. With the purpose determining life-long human tissue, we used (14)C bomb-pulse method. This method takes advantage dramatic increase in atmospheric levels (14)C, produced nuclear bomb tests 1955-1963, which reflected all living organisms. Levels were measured 28 forensic samples Achilles...
Acute exercise induces collagen synthesis in both tendon and muscle, indicating an adaptive response the connective tissue of muscle-tendon unit. However, mechanisms this adaptation, potentially involving collagen-inducing growth factors (such as transforming factor-beta-1 (TGF-beta-1)), well enzymes related to processing, are not clear. Furthermore, possible differential effects specific contraction types on regulation have been investigated. Female Sprague-Dawley rats were subjected 4 days...
In skeletal muscle, an increased expression of insulin like growth factor-I isoforms IGF-IEa and mechano-growth factor (MGF) combined with downregulation myostatin is thought to be essential for training-induced hypertrophy. However, the specific effects different contraction types on regulation these factors in muscle are still unclear, tendon functions myostatin, IGF-IEa, MGF relation training unknown. Female Sprague-Dawley rats were subjected 4 days concentric, eccentric, or isometric ( n...
In skeletal muscle and tendon the extracellular matrix confers important tensile properties is crucially for tissue regeneration after injury. Musculoskeletal adaptation influenced by mechanical loading, which modulates availability of growth factors, including hormone (GH) insulin-like factor-I (IGF-I), may be key importance. To test hypothesis that GH promotes collagen synthesis in musculotendinous tissue, we investigated effects 14 day administration 33-50 microg kg(-1) day(-1)...
The poor regenerative capacity of articular cartilage presents a major clinical challenge and may relate to limited turnover the collagen matrix. However, rate during life is not clear, it debated whether osteoarthritis (OA) can influence it. Using carbon-14 ((14)C) bomb-pulse method, life-long replacement rates were measured in tibial plateau from 23 persons born between 1935 and1997 (15 8 with OA healthy cartilage, respectively). (14)C levels observed showed that, virtually, no matrix...
Important insights concerning the molecular basis of skeletal muscle disuse-atrophy and aging related loss have been obtained in cell culture animal models, but these regulatory signaling pathways not previously studied human muscle. In present study, atrophy was induced by immobilization healthy old young individuals to study time-course transcriptional factors underlying atrophy. The results reveal that irrespectively age, mRNA expression levels MuRF-1 Atrogin-1 increased very initial...
Microdialysis studies indicate that mechanical loading of human tendon tissue during exercise or training can affect local synthesis and degradation type I collagen. Degradation collagen other extracellular matrix proteins is controlled by an interplay between metalloproteinases (MMPs) their inhibitors (TIMPs). However, it unknown whether levels MMPs TIMPs are affected in humans vivo. In the present experiment, six healthy young men performed 1 h uphill (3%) treadmill running. Dialysate was...
Mechanical loading of tissue is known to influence local collagen synthesis, and microdialysis studies indicate that mechanical human tendon during exercise elevates tendinous type I production. Transforming growth factor-β 1 (TGF-β ), a potent stimulator released from cultured fibroblasts in response loading. Thus TGF-β could link synthesis vivo. Tissue levels metabolism markers [procollagen COOH-terminal propeptide (PICP) telopeptide (ICTP)] were measured by peritendinous the Achilles' six...
Tendon tissue and the extracellular matrix of skeletal muscle respond to mechanical loading by increased collagen expression synthesis. This response is likely a secondary effect mechanically induced growth factors, including transforming factor-β1 (TGF-β1) insulin-like factor-I (IGF-I). It not known whether unloading tendon can reduce collagen-inducing factors. Furthermore, coordinated disuse, followed reloading, unclear. Female Sprague-Dawley rats were subjected hindlimb suspension (HS)...
Insulin-like growth factor I (IGF-I) is known to exert an anabolic effect on tendon fibroblast production of collagen. IGF-I's regulation complex and involves six different IGF binding proteins (IGFBPs). Of these, IGFBP-4 -5 could potentially influence the IGF-I in because they both are produced fibroblast; however, response mechanical loading their role tendinous tissue unknown. A splice variant IGF-I, mechano-growth (MGF) upregulated be important for adaptation loaded muscle. However, it...
Acute kicking exercise induces collagen synthesis in both tendon and muscle humans, but it is not known if this relates to increased transcription other matrix genes are regulated. Young men performed 1 h of one‐leg at 67% max workload. Biopsies were taken from the patellar vastus lateralis each leg 2 ( n = 10), 6 11), or 26 10) after exercise. Levels messenger ribonucleic acid mRNA for collagens, noncollagenous proteins, growth factors measured with real‐time reverse polymerase chain...
Overuse Achilles tendinopathy is a common and challenging problem in sports medicine. Little known about the etiology of this disorder, development good animal model for overuse essential advancing insight into disease mechanisms. Our aim was to test previously proposed rat tendon overuse. Ten adult male Sprague-Dawley rats ran on treadmill with 10° incline, 1 h/day, 5 days/wk (17-20 m/min) 12 wk were compared control rats. Histological, mechanical, gene-expression changes measured tendons...
Mechanical loading of tendon cells results in an upregulation mechanotransduction signaling pathways, cell-matrix adhesion and collagen synthesis, but whether unloading removes these responses is unclear. We investigated the response to tension release, with regard matrix proteins, pro-inflammatory mediators phenotypic specific molecules, vitro model where tendon-like tissue was engineered from human cells. Tissue sampling performed 1, 2, 4 6 days after surgical de-tensioning construct. When...
The incidence of Achilles tendinopathy is high and underlying etiology as well biochemical morphological pathology associated with the disease largely unknown. aim present study was to describe differences in chronic tendinopathy. expressions growth factors, inflammatory mediators tendon morphology were determined both chronically diseased healthy parts. Thirty patients randomized an expression-study (n = 16) or a structural-study 14). Biopsies from two areas taken structural parameters:...
Tendons are essential weight-bearing structures that often affected by tendinopathy, which leads to pain and impaired mobility. In healthy Achilles tendons, no significant renewal of the collagen matrix seems occur during adult life, but tendinopathy may lead increased turnover. The carbon-14 ([14C]) bomb pulse method was used measure lifelong replacement rates in tendinopathic tendons (tendinopathic: n = 25, born 1937–1972. Healthy: 10, 1929–1966). As expected, tendon had not been replaced...
Microdialysis studies indicate that mechanical loading of human tendon during exercise elevates type I collagen production in tendon. However, the possibility insertion microdialysis fibers per se may increase local due to trauma has not been explored. Insulin-like growth factor (IGF-I) and its binding proteins (IGFBPs), which are known stimulate animal tendons, regulate translation synthesis. Systemic tissue levels IGF-I, IGFBP, metabolism markers [procollagen COOH-terminal propeptide...
There is a clinical overlap between classic Ehlers-Danlos syndrome (cEDS) and benign joint hypermobility (BJHS), with as the main symptom. The purpose of this study was to investigate role type V collagen mutations tendon pathology in these 2 syndromes. In patients (cEDS, n=7; BJHS, n=8) controls (Ctrl, n=8), we measured patellar ultrastructure (transmission electron microscopy), dimensions (magnetic resonance imaging), biomechanical properties (force ultrasonographic measurements during...