We report the recent emergence of a novel beak and feather disease virus (BFDV) genotype in last remaining wild population critically endangered Orange-bellied Parrot (Neophema chrysogaster). This poses significant threat to recovery species potentially its survival wild. used PCR detect BFDV blood three psittacine (PBFD)–affected fledglings captured as founders for an existing captive breeding program. Complete genome sequence data from one these birds demonstrating 1,993-nucleotide-long...

Quasispecies variants and recombination were studied longitudinally in an emergent outbreak of beak feather disease virus (BFDV) infection the orange-bellied parrot (Neophema chrysogaster). Detailed health monitoring small population size (<300 individuals) this critically endangered bird provided opportunity to track viral replication mutation events occurring a circular, single-stranded DNA over period four years within novel bottleneck population. Optimized PCR was used with different...

Since the characterization of psittacine beak and feather disease (PBFD) in 1984, a wide range avian circoviruses have been discovered with varying pathogenic effects amongst diverse hosts. Until recently these circovirus species were thought to be restricted within Orders such as Psittaciformes for virus (BFDV) Columbiformes pigeon little evidence cross-family transmission or replication. We report naturally occurring novel host switch event self-limiting BFDV infection group rainbow...

Beak and feather disease virus (BFDV) is the dominant pathogen of wild Psittaciformes in Australasia where it has been present for at least 10 million years. The richness psittacine avifauna this region produced a mixture potential hosts resulting competing forces co-evolution, spill-over infection host-switches within parrots, cockatoos lorikeets. Recent evidence shown that all threatened endangered Australian bird species can be infected by BFDV genotypes from any other close or distantly...

Only three wild-bred female Orange-bellied Parrots returned from migration in the 2016/17 breeding season, representing lowest point of a long-term decline. In this context imminent extinction risk we (i) update knowledge population parameters, (ii) critically evaluate current recovery actions, and (iii) identify new management options. We present data season. were only observed at last known site where fire suppression may havecaused shortage natural food. Recently burned habitat elsewhere...

Australia has a comprehensive system of capabilities and functions to prepare, detect respond health security threats. Strong cooperative links coordination mechanisms exist between the human (public health) animal arms in Australia. Wildlife is included this system. Recent reviews both sectors have highlighted Australia's relative strengths detection management emerging zoonotic diseases. However, risks posed by diseases with wildlife as part their epidemiology will almost certainly become...

Abstract Poxviruses are large DNA viruses with varying zoonotic potential, and recognised in a broad range of wildlife. Although poxviruses have been detected kangaroos, their genetic relationships to other animals humans is not well understood. Here, we present novel genome sequence marsupial poxvirus, the Eastern grey kangaroopox virus (EKPV-NSW), isolated from wild eastern kangaroo. In study, histopathologically confirmed epidermal pox lesions were used recover full-length viral perform...

We previously reported that interleukin (IL)-4 treatment of nonobese diabetic (NOD) mice elevates intrapancreatic CCL4 expression and protects from type 1 diabetes. Here, we show antibody neutralization abrogates the ability T-cells IL-4-treated NOD to transfer protection against Intradermal delivery via a plasmid vector stabilized by incorporation Epstein-Barr virus EBNA1/oriP episomal maintenance replicon (pHERO8100-CCL4) beginning at later stages disease progression This was associated...

Characterisation of a complete genome sequence an Australian strain canid alphaherpesvirus 1 (CHV-1) and its phylogenetic relationship with other varicellovirus species.Standard pathology PCR methods were used to initially detect herpesvirus in hepatic tissue from infected 4-week-old Labrador Retriever puppy. The CHV-1 was sequenced using next-generation sequencing technology followed by de novo reference assembly, annotation.The 125 kbp length contained 74 predicted open reading frames...

Objective To discover beak and feather disease virus (BFDV) genotypes in Australian parrots that might threaten vulnerable endangered psittacine bird species. Methods Phylogenetic analyses of new DNA sequence data from birds including the Rep gene (n = 55) nine whole genomes, were compared with all available published BFDV genomes to assess host‐ geographically‐based divergence as well probable host‐switch events. Results Strong support for flexible host‐switching recombination was detected,...

To improve understanding of pathophysiologic processes occurring in green sea turtles ( Chelonia mydas) stranded along the east coast Australia, we retrospectively examined hematologic and biochemical blood parameters 127 admitted to 2 rehabilitation facilities, Dolphin Marine Magic (DMM) Taronga Zoo (TZ), between 2002 2016. The predominant size class presented was small immature animals (SIM), comprising 88% 69% admissions DMM TZ, respectively. Significant differences profiles were noted...

Management of diarrhoea in horses is usually non-specific and supportive. Faecal microbiota transplantations (FMT) are used to manage dysbiosis with diarrhoea. There few studies investigating the effects storage on prepared FMT solutions. This study was an vitro non-randomised controlled experiment that investigated solution preparation faecal microbiota. Fresh faeces were collected from five healthy adult for DNA extraction FMT. From each FMT, seven aliquots extracted immediately after (0...

Case report A mature, captive K refft's river turtle ( E mydura macquarii krefftii ) was presented with severe proliferative and ulcerative lesions of the skin shell. The areas were biopsied histopathological examination demonstrated orthokeratotic hyperkeratosis keratinocytes containing eosinophilic intranuclear inclusions. Molecular diagnostics confirmed presence a herpesvirus in affected tissues. Conclusion This is first recorded case infection an ustralian freshwater species.