A5103026643- MicroRNA in disease regulation
- Protein Hydrolysis and Bioactive Peptides
- Aquaculture disease management and microbiota
- Antimicrobial Peptides and Activities
- Extracellular vesicles in disease
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Invertebrate Immune Response Mechanisms
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Circular RNAs in diseases
- Insect Resistance and Genetics
- Viral Infections and Vectors
- RNA Interference and Gene Delivery
- Polydiacetylene-based materials and applications
- Insect Utilization and Effects
- Insect symbiosis and bacterial influences
Chung-Ang University
2019-2022
Kangwon National University
2015
MicroRNAs (miRNAs) are small non-coding RNAs that contribute to host immune response as post-transcriptional regulation. The current study investigated the biological role of chicken (Gallus gallus) microRNA-200a-3p (gga-miR-200a-3p), using 2 necrotic enteritis (NE) afflicted genetically disparate lines, 6.3 and 7.2, well mechanisms underlying fundamental signaling pathways in chicken. expression gga-miR-200a-3p intestinal mucosal layer NE-induced chickens, was found be upregulated during NE...
Abstract Exosomes are membrane vesicles containing proteins, lipids, DNA, mRNA, and micro RNA (miRNA). Exosomal miRNA from donor cells can regulate the gene expression of recipient cells. Here, Ri chickens were divided into resistant ( Mx /A; BF2 / B21 ) susceptible /G; B13 trait by genotyping genes. Then, infected with H5N1, a highly pathogenic avian influenza virus (HPAIV). purified blood serum for small sequencing. Sequencing data analysed using FastQCv0.11.7, Cutadapt 1.16, miRBase v21,...
The highly pathogenic avian influenza virus (HPAIV) is a threat to the poultry industry as well economy and remains potential source of pandemic infection in humans. Antiviral genes are considered factor for HPAIV resistance. Therefore, this study, we investigated gene expression related cytokine-cytokine receptor interactions by comparing resistant susceptible Ri chicken lines infection.Ri chickens (Mx/A; BF2/B21) (Mx/G; BF2/B13) were selected genotyping Mx dynamin like GTPase (Mx) major...
Defensins are antimicrobial peptides composed of 3 conserved disulfide bridges, a β-sheet, and both hydrophobic cationic amino acids. In this study, we aimed to demonstrate the immunomodulation role avian β-defensin 8 (AvBD8) in chicken macrophage cell line. Chicken AvBD8 stimulated expression proinflammatory cytokines (IL-1β, interferon gamma, IL-12p40) chemokines (CCL4, CXCL13, CCL20) macrophages. Furthermore, by Western blotting immunocytochemistry, confirmed that activated...
To identify immune-related genes in the larvae of white-spotted flower chafers, next-generation sequencing was conducted with an Illumina HiSeq2000, resulting 100 million cDNA reads sequence information from over 10 billion base pairs (bp) and >50× transcriptome coverage. A subset 77,336 contigs created, ∼35,532 sequences matched entries against NCBI nonredundant database (cutoff, e < −5 ). Statistical analysis performed on 35,532 contigs. For profiling immune response, samples were analyzed...
Exosomes are small membrane vesicles that contain proteins and nucleic acids derived from secretory cells mediate intracellular communication. Immune cell-derived exosomes regulate immune responses gene expression of recipient cells. Macrophages recognize viral dsRNA via Toll-like receptor 3, thereby inducing the activation transcription factors such as interferon regulatory factor 3 nuclear kappa-light-chain-enhancer activated B (NF-κB). In this study, we aimed to identify immunomodulatory...
Abstract Background Defensins are antimicrobial peptides composed of three conserved disulfide bridges, a β-sheet, and both hydrophobic cationic amino acids. In this study, we aimed to demonstrate the immunomodulation role avian β-defensin 8 (AvBD8) in chicken macrophage cell line.Results Chicken AvBD8 stimulated expression proinflammatory cytokines (interleukin (IL)-1β, interferon-γ, IL-12p40) chemokines (CCL4, CXCL13, CCL20) macrophages. Furthermore, by western blotting...
Abstract Background: Defensins are antimicrobial peptides composed of three conserved disulfide bridges, a β-sheet, and both hydrophobic cationic amino acids. In this study, we aimed to demonstrate the immunomodulation role avian β-defensin 8 (AvBD8) in chicken macrophage cell line. Results: Chicken AvBD8 stimulated expression proinflammatory cytokines (interleukin (IL)-1β, interferon-γ, IL-12p40) chemokines (CCL4, CXCL13, CCL20) macrophages. Furthermore, by western blotting...