A5088108160- Phytochemicals and Antioxidant Activities
- Postharvest Quality and Shelf Life Management
- Biochemical Analysis and Sensing Techniques
- Pesticide Residue Analysis and Safety
- Isotope Analysis in Ecology
- Mycotoxins in Agriculture and Food
- Nanocomposite Films for Food Packaging
- Cassava research and cyanide
- Plant Gene Expression Analysis
- Ziziphus Jujuba Studies and Applications
- Advanced Chemical Sensor Technologies
- Antibiotics Pharmacokinetics and Efficacy
- Ginseng Biological Effects and Applications
- Freezing and Crystallization Processes
- Mangiferin and Mango Extracts
- Aquaculture Nutrition and Growth
- Insect-Plant Interactions and Control
- Bioenergy crop production and management
- Reproductive biology and impacts on aquatic species
- Electrospun Nanofibers in Biomedical Applications
- GABA and Rice Research
- Wheat and Barley Genetics and Pathology
- Plant Pathogens and Resistance
- Advanced biosensing and bioanalysis techniques
- Tea Polyphenols and Effects
Hainan Agricultural School
2024
Hainan Provincial Academy of Agricultural Sciences
2022-2024
Due to the lack of comprehensive evaluation all metabolites in wampee, metabolic reasons for taste differences are unclear. Here, two local varieties YF1 (sweet taste) and YF2 (sweet-sour taste), were selected quality analysis, followed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based widely targeted metabolomic analysis. clearly separated principal component analysis (PCA) cluster 449 different between cultivars, including 29 carbohydrates organic...
Chlorine dioxide (ClO2) gas has attracted considerable attention due to its safety and efficiency. In this study, we successfully developed a color-variable ClO2 slow-releasing card for postharvest litchi. The optimal was prepared as follows: Card A soaked in 2.5 mol/L NaClO2 0.3 CaCl2. B 1 oxalic acid, CaCl2, natural pigment. Finally, cards were dried adhered using 60% gelatin. releasing time of the more than 120 h at 5–25 °C, it could change color from dark yellow white. qualities 3 kg...
The metabolic reasons for rambutan taste variations during maturity are unknown. Here, we obtained a unique cultivar Baoyan No.2 (BY2) with strong yellow pericarp and excellent taste, the sugar-acid ratios of which ranged from 21.7 to 94.5 maturation. Widely targeted metabolomics analysis was performed reveal behind these variations. results showed that 51 metabolites were identified as common different (DMs), including 16 lipids, 12 amino acids others. Among them, abundance level...
The metabolite-caused taste variation during rambutan maturation is unknown due to a lack of systematic investigation all components. In this study, three growing stages, including unripe (S1), half-ripe (S2), and full-ripe (S3) BY2 BY7 rambutans were compared profiled by UPLC-MS/MS-based widely targeted metabolomics analysis. We demonstrated that the sugar-acid ratios two greatly improved between S2 S3 stages. A total 821 metabolites identified, 232, 205, 204, 12 differential (DMs) in...
Triflumezopyrim, a novel mesoionic insecticide used to control planthoppers, is potential substitute for imidacloprid. In this study, triflumezopyrim and imidacloprid residues in rice were determined using quick, easy, cheap, effective, rugged, safe procedure combined with ultra-high-performance liquid chromatography-tandem mass spectrometry. The limit of quantification both was 0.01 mg kg-1, the average recovery values 94-104% 91-106%, relative standard deviations (RSDs) 1.1-1.4% 2.1-3.4%...
This study aimed to understand how temperatures differentially impact the crucial quality indices and metabolites in rambutan during storage. Rambutan browned quickly at room temperature from 0 d (control). After ten days 5 ℃, browning index lightness were 4.2% 147.5%, compared with stored 1 which was best achieved. An UPLC-MS/MS performed uncover metabolism underlying those differences, followed by analysis of KEGG pathways. Results showed that 276 expressed (DEMs) screened enriched 18 The...
Mycotoxins exposure from food can trigger serious health hazards. This study aimed to establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous detection of 44 mycotoxins in fruits and their products, followed by dietary risk assessment. The optimized UPLC-MS/MS exhibited a good linear relationship with correlation coefficients ≥ 0.99041. limits (LOD) quantification (LOQ) were within range 0.003 ∼ 0.700 μg/kg 0.01 2.00 μg/kg,...
Summary The high content of cyanogenic glycosides (CG) in cassava storage tubers seriously affects human food safety. CG play crucial roles plant growth and development can protect leaves from being masticated by herbivorous predators. Nevertheless, the regulatory mechanism biosynthesis, which results a low leaves, remains poorly understood. Here, yeast one-hybrid assay was performed using mixed cDNA library roots as prey promoter MeCYP79D2 bait. MeCYP79D2, cytochrome P450 protein, is...
So far, the metabolic differences between tree-ripened and postharvest-ripened mangoes have largely remained unexplored. The aim of this study was to evaluate chemical composition nutrient substances in subjected different ripening methods. An untargeted metabolomic approach based on ultra performance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) carried out investigate artificially ripened naturally mangoes. principal component analysis results...
Stylosanthes is an excellent tropical leguminous forage. Anthracnose caused by Colletotrichum gloeosporioides a major limiting factor in production, which can lead to reduced forage and seed production decreased quality. How quickly screen resistant parents varieties of that are anthracnose has become urgent problem be solved production. In this study, the high-performance liquid chromatography detection technology was established for simultaneous analysis content salicylic acid, triethyl...
Mycotoxins exposure from food can trigger serious health hazards. This study aimed to establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous detection of 44 mycotoxins in fruits and their products, followed by dietary risk assessment. The optimized UPLC-MS/MS exhibited a good linear relationship with correlation coefficients ≥ 0.99041. limits (LOD) quantification (LOQ) were within range 0.003~0.700 μg/kg 0.01~2.00 μg/kg,...
Recently, there have been increasing safety concerns about the illegal abuse of quinolone in soybean sprouts. This study aimed to establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for simultaneous detection 16 quinolones (QNs) sprouts, and then reveal their degradation characteristics. The samples were extracted with acetonitrile (with 1% formic acid), purified by a C18 adsorbent, separated ACQUITY UPLC BEH (1.7 μm, 2.1 mm × 100 mm)...
The advantages of genetic modification and preferable physicochemical qualities make nanobody (Nb) easy to develop a sensitive stable immunosensor platform. Herein, an indirect competitive chemiluminescence enzyme immunoassay (ic-CLEIA) based on biotinylated Nb was established for the quantification diazinon (DAZ). anti-DAZ Nb, named Nb-EQ1, with good sensitivity specificity, obtained from immunized library via phage display technique, where molecular docking results indicated that hydrogen...
Biogenic amines (BAs) produced by microbial decarboxylation of amino acids are crucial toxic nitrogenous compounds in fish. An optimized UPLC–MS/MS method with simple pretreatment was established to detect 14 BAs both raw (CK) and deep-fried (DF) hairtails. This exhibited a good linear relationship relative standard deviations ranging from 0.5 5.2. The total CK DF hairtails decreased sharply 338.2 25.3 mg/kg on the 9th day, respectively. Four BAs, including cadaverine (CAD), histamine (HIS),...