A5061445953- Advanced biosensing and bioanalysis techniques
- Electrochemical Analysis and Applications
- DNA and Nucleic Acid Chemistry
- Electrochemical sensors and biosensors
- Molecular Junctions and Nanostructures
- Conducting polymers and applications
- Surface Roughness and Optical Measurements
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Sensors
- Bone Tissue Engineering Materials
- Gout, Hyperuricemia, Uric Acid
- Metabolism and Genetic Disorders
- Nanopore and Nanochannel Transport Studies
- RNA and protein synthesis mechanisms
- Blood properties and coagulation
- Laser Applications in Dentistry and Medicine
- Lipid Membrane Structure and Behavior
- Molecular Sensors and Ion Detection
- Force Microscopy Techniques and Applications
- Dental Implant Techniques and Outcomes
- Laser Material Processing Techniques
- Laser-Ablation Synthesis of Nanoparticles
- Peripheral Artery Disease Management
- Analytical chemistry methods development
- Minerals Flotation and Separation Techniques
University of California, Irvine
2024
Czech Academy of Sciences
2006-2023
Czech Academy of Sciences, Institute of Biophysics
2010-2023
Masaryk University
2000-2011
University of Eastern Finland
2011
Stripping voltammetric determination of purine bases in the presence copper ions at mercury, amalgam, or carbon-based electrodes has recently been utilized analysis DNA synthetic oligodeoxynucleotides (ODNs). Here we report on copper-enhanced label-free anodic stripping detection guanine and adenine acid-hydrolyzed anodically oxidized boron-doped diamond electrode (AO-BDDE). The AO-BDDE was successfully applied a three-electrode microcell which an ∼50 μL drop analyte solution can be...
Using a mechanically grinded pyrolytic graphite electrode in edge orientation, sensitive electrochemical method was developed for simultaneous determination of uric acid (UA), xanthine (XAN), hypoxanthine (HYP) (products purine catabolism human), allopurinol (ALO), and oxypurinol (OXY) (a drug used treatment disorders its metabolite, respectively). It is demonstrated that differential pulse voltammetry connection with this can serve as simple efficient tool monitoring transformation...
In this review we briefly summarize the results of impedance measurements (dependence differential capacitance C and/or Z electrode double layer on potential E, C-E curves) or frequency (Electrochemical Impedance Spectroscopy-EIS) nucleic acids at mercury film (MFE). Mercury was plated either a glassy carbon (GCE) pyrolytic graphite (PGE). The dependence pseudocapacitance redox peak echinomycin concentration and ac voltage measured in bulk solution. Interaction single- double-stranded DNA...
Abstract Mechanically grinded edge‐plane pyrolytic graphite electrode (g‐PGEe) was applied in voltammetric analysis of purine nucleobases, acid‐hydrolyzed synthetic oligodeoxynucleotides and a nonhydrolyzed plasmid DNA. Properties the mechanically electrodes these analytical applications were compared with some other carbon types. We show that surface grinding 15‐μm SiC particles resulted remarkable improvement oxidation signals bases no addition copper ions. Addition ions, causing strong...
Adsorption of human plasma fibrinogen (HPF) on 6 differently treated titanium samples (polished, polished and etched, 4 carbide coatings produced by using plasma-enhanced chemical vapour deposition (PECVD) method) is investigated diffractive optical element (DOE) sensor. Permittivity (susceptibility) change fluctuation in roughness (R(opt)) surface the presence background electrolyte without with HPF molecules are sensed DOE sensor ellipsometry. Correlation between transmitted light...
Abstract The determination of adenine (Ade), adenosine (Ado) and hydrolyzed (h‐Ado) in the presence copper(II) ions is described by cyclic (CV) elimination voltammetry with linear scan (EVLS) connection adsorptive stripping technique. Signals copper‐adenine complex were measured on a hanging mercury drop electrode (HMDE) buffered solutions different pH. differences electrochemical behavior Ade Ado found not only dependence copper ions, rate, concentration pH, but also accumulation time...
We present a simple, cost-effective design for amplifying oligodeoxynucleotide (ODN) sensing, in microliter ODN volumes containing copper ions, by solution streaming (bubbling). The inert gas (bubbling) at constant pressure of 0.04 bar drives the motion 30-μL droplet three-electrode circuit (inverted drop microcell), and presence ions offers an ∼50-times improvement detection samples. ODNs carbon paste electrode is based on enhancement oxidation peaks purine bases (adenine guanine) anodic...