A5064010443- Electrochemical Analysis and Applications
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- RNA and protein synthesis mechanisms
- Analytical Chemistry and Sensors
- Mass Spectrometry Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Glycosylation and Glycoproteins Research
- DNA and Nucleic Acid Chemistry
- Lipid Membrane Structure and Behavior
- Molecular Junctions and Nanostructures
- Carbohydrate Chemistry and Synthesis
- Biosensors and Analytical Detection
- Parkinson's Disease Mechanisms and Treatments
- RNA Interference and Gene Delivery
- Advanced Chemical Sensor Technologies
- Monoclonal and Polyclonal Antibodies Research
- Biotin and Related Studies
- Galectins and Cancer Biology
- Nanopore and Nanochannel Transport Studies
- Photosynthetic Processes and Mechanisms
- Molecular Biology Techniques and Applications
- Conducting polymers and applications
- thermodynamics and calorimetric analyses
- Biochemical and biochemical processes
Czech Academy of Sciences
2014-2025
Czech Academy of Sciences, Institute of Biophysics
2016-2025
Akademie (Czechia)
2024
Czech Academy of Sciences, Institute of Analytical Chemistry
2010
In-Q-Tel
2010
Institute of Biophysics
2009
Comenius University Bratislava
2003-2008
Prostate cancer (PCa) is the second most common cancer. In this paper, isolation and properties of exosomes as potential novel liquid biopsy markers for early PCa diagnosis are investigated using two prostate human cell lines, i.e., benign (control) line RWPE1 carcinoma 22Rv1. Exosomes produced by both lines characterised various methods including nanoparticle-tracking analysis, dynamic light scattering, scanning electron microscopy atomic force microscopy. addition, surface plasmon...
Abstract Present proteomics and biomedicine require sensitive analytical methods for all proteins. Recent progress in electrochemical analysis of peptides proteins based on their intrinsic electroactivity is reviewed. Tyrosine and/or tryptophan‐containing are oxidizable at carbon electrodes. At mercury electrodes (about 13 >25 were tested) produce chronopotentiometric peak H nanomolar concentrations. This to changes protein structure. Microliter sample volumes sufficient the analysis....
Parkinson's disease (PD) is associated with the formation and deposition of amyloid fibrils protein α-synuclein (AS). It has been proposed that oligomeric intermediates on pathway to fibrilization rather than themselves are pathogenic agents PD, but efficient methods for their detection lacking. We have studied interfacial properties wild-type AS course its aggregation in vitro using electrochemical analysis dynamic light scattering. The oxidation signals tyrosine residues at carbon...
We developed an innovative electrochemical method for monitoring conformational transitions in proteins using constant current chronopotentiometric stripping (CPS) with dithiothreitol-modified mercury electrodes. The was applied to study the effect of oncogenic mutations on DNA-binding domain tumor suppressor p53. CPS responses wild-type and mutant p53 showed excellent correlation structural stability data provided additional insights into differential dynamic behavior proteins. Further, we...
Dithiothreitol (DTT)−mercury and DTT−solid amalgam electrodes are proposed for protein microanalysis by means of constant current chronopotentiometric stripping (CPS). At the DTT-modified hanging mercury drop electrode (DTT-HMDE), proteins at nanomolar concentrations produce CPS peak H, which is due to catalyzed hydrogen evolution. Self-assembled monolayers (SAMs) DTT surface protected surface-attached from electric field-driven denaturation, but did not interfere with electrocatalysis....
A simple and effective method to prepare an enzyme electronic biosensor by immobilizing nanoparticles directly onto the gold electrode surface is described; prepared horseradish peroxidase have been successfully used develop reagentless biosensors for H2O2 detection without promoters mediators offer great potential enzyme-based biosensors.
Gold and carbon electrodes have been largely used as transducers in protein DNA sensors arrays. Liquid mercury electrodes, with potential windows allowing detection of reduction processes at highly negative potentials, were considered useless such Here, we show that solid amalgam electrode (SAE) arrays can be prepared a substitution liquid the analysis above biomacromolecules. Vacuum metal sputtering on glass substrate, photolithography, galvanic formation for fabrication an inexpensive...
Using constant current chronopotentiometry we showed that in 50 mM sodium phosphate (pH 7) bovine serum albumin and some other proteins were not significantly denatured at a bare mercury electrode while higher concentrations they underwent electric field-driven denaturation on the surface.
In contrast to previous reports claiming bovine serum albumin (BSA) denaturation at mercury surfaces, recently it has been shown that BSA and other proteins do not denature as a result of adsorption the electrodes alkaline neutral pH values. this range, constant current chronopotentiometry (CPS) with or solid amalgam can be used distinguish between native, denatured damaged BSA. Here we show acid values (around 4.5) native yield almost same CPS responses suggesting electrode surface. Under...
Abstract Osmium tetroxide complexes with nitrogen ligands [Os(VIII)L] have been widely applied as probes of the DNA structure and electroactive labels DNA. Here we describe electrochemical behavior Os(VIII)2,2‐bipyridine (Os, bipy)‐base‐labeled nucleosides. We show that label can be introduced also in nucleoside ribose residues using six‐valent osmium complex. Cyclic voltammograms sugar‐Os(VI)‐modified ribosides are similar but not identical to those base‐modified ribosides. Our results...