A5011809352- Glycosylation and Glycoproteins Research
- Enzyme Catalysis and Immobilization
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Protein purification and stability
- Extracellular vesicles in disease
- Galectins and Cancer Biology
- MXene and MAX Phase Materials
- Advanced Biosensing Techniques and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Amino Acid Enzymes and Metabolism
- Lipid Membrane Structure and Behavior
- thermodynamics and calorimetric analyses
- Microfluidic and Capillary Electrophoresis Applications
- Microbial metabolism and enzyme function
- Enzyme Production and Characterization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Memory and Neural Computing
- Graphene and Nanomaterials Applications
- Electrohydrodynamics and Fluid Dynamics
- Analytical Chemistry and Sensors
- Analytical Chemistry and Chromatography
Slovak Academy of Sciences
2016-2025
Institute of Chemistry of the Slovak Academy of Sciences
2015-2024
Institute of Plant Genetics and Biotechnology of the Slovak Academy of Sciences
2018
Abstract Impedimetric affinity biosensors are, without any doubt, among the most sensitive analytical devices available, offering low limits of detection and wide linear response ranges. There however, only a few papers detailing application impedimetric for analysis clinically relevant samples with due clinical performance. The fact that these have not found their way to commercial or use date might be surprising, since an electrochemical assay platform based on portable potentiostats is...
Two-dimensional layered nanomaterial Ti3C2TX (a member of the MXene family) was used to immobilise enzyme sarcosine oxidase fabricate a nanostructured biosensor. The device applied for detection sarcosine, potential prostate cancer biomarker, in urine first time. morphology and structures have been characterised by atomic force microscopy (AFM) scanning electron (SEM). Electrochemical measurements, SEM AFM analysis revealed that interfaced with chitosan is an excellent support immobilisation...
Ultrasensitive impedimetric lectin biosensors recognizing different glycan entities on serum glycoproteins were constructed. Lectins immobilized a novel mixed self-assembled monolayer containing 11-mercaptoundecanoic acid for covalent immobilization of lectins and betaine terminated thiol to resist nonspecific interactions. Construction based Concanavalin A (Con A), Sambucus nigra agglutinin type I (SNA), Ricinus communis (RCA) polycrystalline gold electrodes was optimized characterized with...
Prostate cancer (PCa) is the second most common cancer. In this paper, isolation and properties of exosomes as potential novel liquid biopsy markers for early PCa diagnosis are investigated using two prostate human cell lines, i.e., benign (control) line RWPE1 carcinoma 22Rv1. Exosomes produced by both lines characterised various methods including nanoparticle-tracking analysis, dynamic light scattering, scanning electron microscopy atomic force microscopy. addition, surface plasmon...
Abstract MXenes are a new group of 2D nanomaterials with fascinating properties including high electrical conductivity, hydrophilic nature, easily tunable structure and surface area. This is why MXene modified interfaces extremely promising for the preparation sensitive electrochemical biosensors. While there numerous reports on MXene‐based enzymatic biosensors detection wide range analytes, application construction affinity in its infancy. The review article summarizes current state‐of...
Exosomes are considered to be a rich source of biomarkers, hence in this article we examine the best procedure for their isolation. We several isolation procedures, exosome storage conditions and other affecting production by prostate cell lines. selected four different commercially available kits based on principles achieve isolation, being magnetic-based. In addition, found at - 20 °C good storing isolated exosomes that were produced from cancerous line 22Rv1 much greater amounts than...
A label-free ultrasensitive impedimetric biosensor with lectin immobilised on graphene oxide (GO) for the detection of glycoproteins from 1 aM is shown here.
We present here comparison of a build-up two ultrasensitive lectin biosensors based on 2D or 3D architecture. A biosensor was prepared by covalent immobilisation Sambucus nigra agglutinin (SNA) recognising sialic acid directly mixed self-assembled monolayer (SAM) planar gold surfaces. SNA SAM layer formed nanoparticles. Surface plasmon resonance technique allowed to follow kinetics (1:1 mixture 11-mercaptoundecanoic and 6-mercaptohexanol) formation bare electrode an modified 5 nm 20...
Impedimetric lectin biosensors capable of recognizing two different carbohydrates (galactose and sialic acid) in glycans attached to antibodies isolated from human serum were prepared. The first step entailed the modification a gold surface by self-assembled monolayer (SAM) deposited solution containing carboxybetaine-terminated thiol applied subsequent covalent immobilization lectins resist nonspecific protein adsorption. In next step, Sambucus nigra agglutinin (SNA) or Ricinus communis...
Abstract Exosomes are naturally produced biological nanoparticles secreted by cells into body fluids and responsible for intercellular communication. can also carry cargo affecting neighbouring forming pre‐metastatic niches. Hence, exosomes behind localised tumour development, progression, but the induction of distant tumours metastasis. A substantially higher cellular activity results in production a greater number than normal, healthy cells. Therefore, present serve as good diagnostic...