To prepare peptides with high angiotensin-converting enzyme (ACE) inhibitory (ACEi) activity, Alcalase was screened from five proteases and employed to protein hydrolysate (TMH) of skipjack tuna (Katsuwonus pelamis) milts. Subsequently, 10 novel ACEi were isolated the high-ACEi activity TMH identified as Tyr-Asp-Asp (YDD), Thr-Arg-Glu (TRE), Arg-Asp-Tyr (RDY), Thr-Glu-Arg-Met (TERM), Asp-Arg-Arg-Tyr-Gly (DRRYG), Ile-Cys-Tyr (ICY), Leu-Ser-Phe-Arg (LSFR), Gly-Val-Arg-Phe (GVRF),...

Hepatitis C virus (HCV) NS5B is RNA-dependent RNA polymerase (RdRP), the essential catalytic enzyme for HCV replication. Recently, NS5A has been reported to be important establishment of replication in vitro by adaptive mutations, although its role viral remains uncertain. Here we report that purified bacterial recombinant and directly interact with each other vitro, detected glutathione S-transferase (GST) pull-down assay. Furthermore, complex formation these proteins transiently...

Acid soluble collagen (ASC) from scales of croceine croaker (ASC-C) was successfully isolated with the yield 0.37% ± 0.08% (dry weight basis), and characterized as type I on basis amino acid analysis electrophoretic pattern. The antioxidant hydrolysate ASC-C (ACH) prepared through a two-stage in vitro digestion (4-h trypsin followed by 4-h pepsin), three peptides (ACH-P1, ACH-P2, ACH-P3) were further ACH using ultrafiltration, gel chromatography, RP-HPLC, their sequences identified...

Influence of amino acid compositions and peptide profiles on antioxidant capacities two protein hydrolysates from skipjack tuna (Katsuwonus pelamis) dark muscle was investigated. Dark muscles were hydrolyzed using five separate proteases, including pepsin, trypsin, Neutrase, papain Alcalase. Two hydrolysates, ATH NTH, prepared Alcalase respectively, showed the strongest further fractionated ultrafiltration gel filtration chromatography. fractions, Fr.A3 Fr.B2, isolated strong radical...

ABSTRACT Hepatitis C virus (HCV) NS5A has been reported to be important for the establishment of replication by adaptive mutations or localization, although its role in viral remains unclear. It was previously that interacts with NS5B via two regions isolate JK-1 and modulates activity RdRp (Y. Shirota et al., J. Biol. Chem., 277: 11149-11155, 2002), but biological significance this interaction not determined. In study, we addressed effect on HCV RNA an replicon system derived from M1LE (H....

The antioxidant activities of Methanol/Chloroform (MC) extracts and fractions five brown algae (Sargassum fusiforme, Sargassum kjellmanianum, Sargassum. pallidum, thunbergii horneri) from China were evaluated through DPPH/hydroxyl radical-scavenging activity reducing power. Total phenolic content was investigated using Folin–Ciocalteau reagent. MC extract S. kjellmanianum showed higher than other seaweeds. However, the all seaweeds lower DPPH Gallic Acid (GA) ascorbic acid (AscA) at 50...

Inspired by DPPH radical scavenging assay, an efficient method had been developed to acquire the hydrolysate from Sphyrna lewini muscle protein (SPH) orthogonal L9(3)4 test. Under optimal proteolysis parameters (proteolysis time 2 h, total enzyme dose 1.2%, temperature 60 °C and pH 6), rate of SPH reached 91.27 ± 2.69% at concentration 25 mg/mL. Proteolysis showed significant effects on preparation (p < 0.05). One peptide (SAP) was isolated using ultrafiltration, anion-exchange...

Enzymatic proteolysis of food proteins is considered a promising method to generate antibacterial peptides. The objective the present study was isolate and characterize peptide fraction from pepsin hydrolysate half-fin anchovy (Setipinna taty) with activity against Escherichia coli. most active HAHp2-3-I isolated by series chromatographic methods, including Sephadex G-25 chromatography, reverse high-performance liquid chromatography (RP-HPLC) Source 5RPC ST. Peptides identification carried...

For full use of the by-products during Siberian sturgeon ( Acipenser baerii ) processing, gelatin was extracted from cartilages using hot water method, and its physico-chemical properties protective function on ultraviolet-A injured human skin fibroblasts (HFSBs) were measured. Using single-factor orthogonal experiments, conditions for extracting cartilage optimized as extraction time 7 h, pH 9, material-to-liquid ratio (g/ml) 1:5, temperature 45°C. The prepared (TCG) with a yield 28.8 ±...

HCV NS5B is an RNA-dependent RNA polymerase (RdRP), a central catalytic enzyme for replication, which has the "palm and fingers" substructure. We recently identified five novel residues critical RdRP activity (Qin, W., Yamashita, T., Shirota, Y., Lin, Wei, Murakami, S. (2001)Hepatology 33, 728–737). Among them, GLU-18 His-502, far from center, may be involved in conformational change(s) as addressed some palm fingers enzymes. examined possibility that oligomerized, we could detect...

The efficient and non-destructive evaluation of Yongchuan Xiuya tea quality represents a key advancement in the industry. Near-infrared spectroscopy (NIRS), non-invasive analytical technology, allows for acquisition spectral data while preserving sample integrity. Through preprocessing employing synergy interval partial least squares (siPLS) method to identify characteristic regions, principal component analysis (PCA) is applied, followed by development Jordan–Elman artificial neural network...

The realization of natural resource value serves as a critical entry point for advancing rural revitalization within the framework ecological civilization construction, representing an essential approach to balancing conservation and economic development in national parks. Based on clarifying logical relationship driving mechanisms between revitalization, this paper employs field observation in-depth interviews using Baishanzu National Park case study analyze how general control zones parks...

Polysaccharides are very promising molecules in the field of pharmacotherapy. Knowing this, aim this study was to extract, characterize, and evaluate action polysaccharides noni juice, using biological models Type 2 diabetes mellitus processes. In study, one polysaccharide named NJSPd-1 separated from fermented fruit juice. The characterization assay showed that had a molecular weight (Mw) 18,545 Da. consisted galacturonic acid, galactose, rhamnose, arabinose, with molar ratio...

Mussels are a kind of economically valuable ocean bivalve shellfish. It has short harvest period and is susceptible to contamination during storage processing. Having proper preservation methods critical prevent quality deterioration. However, the effect low voltage variable frequency electric field compound preservative on freshness steamed mussels in ice-temperature still unknown. We utilized method coefficient variation weighting calculate overall scores stored under different conditions....

Achieving an excellent energy storage performance, together with a high cycling reliability, is desirable for expanding the technological applications of ferroelectric dielectrics. However, in well-crystallized material, concomitant polarizability and low polarization-saturation field have led to square-shaped polarization-electric loop, fatally impairing both recoverable density (<em>W</em>_rec) efficiency (<em>η</em>). Nanocrystalline films macroscopically amorphous structure...

The excellent energy storage performances of dielectric materials, a high density and efficiency, the stability in wide range temperature, frequency cycling time, are surely desirable for devices. A trade-off relationship between polarization breakdown strength, however, limits enhancement properties materials. To effectively boost efficiency capacitors, by inserting BiFeO3 layer into BaTiO3 film present case, symmetric BaTiO3/BiFeO3/BaTiO3 tri-layer heterostructure with...