A5065145337- Contact Dermatitis and Allergies
- Animal testing and alternatives
- Allergic Rhinitis and Sensitization
- Advancements in Transdermal Drug Delivery
- Skin Protection and Aging
- Effects and risks of endocrine disrupting chemicals
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bee Products Chemical Analysis
- Dermatology and Skin Diseases
- Computational Drug Discovery Methods
- Pesticide Exposure and Toxicity
- Antioxidant Activity and Oxidative Stress
- Spectroscopy and Chemometric Analyses
- Immunotoxicology and immune responses
- Renal function and acid-base balance
- Digital Imaging in Medicine
- Nanoplatforms for cancer theranostics
- Analytical Chemistry and Chromatography
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical effects in animals
- Eicosanoids and Hypertension Pharmacology
- Cholesterol and Lipid Metabolism
- Steroid Chemistry and Biochemistry
- Free Radicals and Antioxidants
- Peroxisome Proliferator-Activated Receptors
Unilever (United Kingdom)
2011-2024
University of Bedfordshire
1999-2023
Abstract Next-Generation Risk Assessment is defined as an exposure-led, hypothesis-driven risk assessment approach that integrates new methodologies (NAMs) to assure safety without the use of animal testing. These principles were applied a hypothetical 0.1% coumarin in face cream and body lotion. For purpose evaluating NAMs, existing human data on excluded. Internal concentrations (plasma Cmax) estimated using physiologically based kinetic model for dermally coumarin. Systemic toxicity was...
Next Generation Risk Assessment (NGRA) is a procedure that integrates new approach methodologies (NAMs) to assure safety of product without generating data from animal testing. One the major challenges in application NGRA consumer products how extrapolate vitro points departure (PoDs) human exposure level associated with use. To bridge gap, physiologically based kinetic (PBK) modelling routinely used predict systemic (Cmax or AUC) external exposures. A novel framework was developed for...
Among the factors determining propensity of a chemical to induce skin allergy are penetration into and kinetics ingress. Confocal Raman spectroscopy can provide such information as it enables direct, spatially resolved measurement any uptake. Several chemicals be monitored at once, method is non-destructive (light in, light out) so that kept intact for repeated continuous measurement. was used follow 2.5 weight percent <i>trans</i>-cinnamaldehyde its delivery vehicle in vitro, up...
Combined with in vitro bioactivity data, physiologically based kinetic (PBK) models has increasing applications next generation risk assessment for animal-free safety decision making. A tiered framework of building PBK such application been developed complexity and refinements, as model parameters determined silico, vitro, human pharmacokinetic data become progressively available. modelling widely applied oral/intravenous administration, but less so on topically chemicals. Therefore, topical...
The mechanistic understanding of skin penetration underpins the design, efficacy and risk assessment many high-value products including functional personal care products, topical transdermal drugs. Stimulated Raman scattering (SRS) microscopy, a label free chemical imaging tool, combines molecular spectroscopy with submicron spatial information to map distribution chemicals as they penetrate skin. However, quantification is hampered by significant interference from signals constituents. This...
Development of risk assessment methods for skin sensitization in the absence toxicological data generated animals represents a major scientific and technical challenge. The first step human induction is transport sensitizer from applied dose on surface to epidermis, where innate immune activation occurs. Building previous development time course vitro permeation assay, new kinetic 10 sensitizers 2 nonsensitizers are reported. Multicompartmental modeling has been analyze determine candidate...
Synopsis Photocontact allergy, an acquired altered reactivity of the skin to light in presence a photosensitizer, has for many years been considered be delayed‐type hypersensitivity. The response postulated as being mediated via formation protein‐photoallergen conjugate acting complete antigen. purpose this paper is bring together evidence at molecular level which supports theory photoallergy. All photoallergens studied so far have shown able bind proteins under influence ultraviolet light....
Noncellular and cellular in vitro models for predicting intestinal absorption were used to investigate the transport metabolism of parabens. The biomimetic artificial membrane permeability assay (BAMPA) was constructed by impregnating a lipid solution on hydrophobic filter. Caco-2 cells at passage numbers 65 80 cultured either accelerated 3-day Biocoat system or standard 21-day Transwell cell culture system. Paraben across BAMPA showed parabolic relationship. lowest log P (p-hydroxybenzoic...
Allergic Contact Dermatitis (ACD; chemical-induced skin sensitisation) represents a key consumer safety endpoint for the cosmetics industry. At present, animal tests (predominantly mouse Local Lymph Node Assay) are used to generate sensitisation hazard data use in risk assessments. An testing ban on chemicals be will come into effect European Union (EU) from March 2009. This is also linked an EU marketing products containing any ingredients that have been subsequently tested animals, 2009 or...
In vitro skin absorption methods exist in Organisation for Economic Co-operation and Development (OECD) guideline form (No. 428) are used to estimate the degree of systemic penetration chemicals through skin. More detailed kinetics permeation compartments not described well by existing methods. This study was designed assess practical feasibility generating compartmental (stratum corneum/epidermal/dermal) disposition kinetic data topically applied chemicals. For chemically induced effects...
p-Hydroxybenzoate ester (paraben) preservatives are used in numerous orally administered products. The recognized route of metabolism for parabens is hydrolysis to p-hydroxybenzoic acid followed by conjugation and excretion. However, the presence alcohols, a presystemic transesterification pathway not previously reported human intestine can occur. Using intestinal (Caco-2) cells, it was observed that reduced markedly ethanol concentrations occur intestine, 0.25–0.5% (v/v). Ethanol 1.0–2.5%...
Abstract Estimating human exposure in the safety assessment of chemicals is crucial. Physiologically based kinetic (PBK) models which combine information on exposure, physiology, and chemical properties, describing absorption, distribution, metabolism, excretion (ADME) processes a chemical, can be used to calculate internal metrics such as maximum concentration area under concentration-time curve plasma or tissues test next-generation risk assessment. This article demonstrates development...