Manar Makhoul

ORCID: 0000-0003-4970-209X
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About
Contact & Profiles
Research Areas
  • Wheat and Barley Genetics and Pathology
  • Plant Disease Resistance and Genetics
  • Genetic Mapping and Diversity in Plants and Animals
  • Plant nutrient uptake and metabolism
  • Genomics and Phylogenetic Studies
  • Chromosomal and Genetic Variations
  • Genetics and Plant Breeding
  • Plant Genetic and Mutation Studies
  • Agricultural pest management studies
  • Genetic and Environmental Crop Studies
  • Rice Cultivation and Yield Improvement
  • Crop Yield and Soil Fertility
  • Plant Pathogens and Resistance
  • Genetic diversity and population structure

Justus-Liebig-Universität Gießen
2020-2025

Conversion of SNP chip assays into locus-specific KASP markers requires adapted strategies in polyploid species with high genome homeology. Procedures are exemplified by QTL-associated SNPs hexaploid wheat. Kompetitive allele-specific PCR (KASP) commonly used marker-assisted commercial plant breeding due to their cost-effectiveness and throughput for sample volumes. However, conversion trait-linked from array-based detection technologies is particularly challenging crop species, the presence...

10.1007/s00122-020-03608-x article EN cc-by Theoretical and Applied Genetics 2020-06-04

ABSTRACT Vicine and convicine (v‐c) pose significant challenges to the global use of faba beans, causing favism in susceptible individuals. Developing low v‐c beans is crucial enhance their utilization safety, but progress has been hindered by insufficient genomic tools for marker‐assisted selection. This challenge linked complex nature responsible locus, which makes marker development difficult. While a two‐base pair insertion/deletion VC1 correlates with phenotype, converting this variant...

10.1111/pbr.13276 article EN cc-by Plant Breeding 2025-03-09

Abstract Key message PHOTOPERIOD-1 homoeologous gene copies play a pivotal role in regulation of flowering time wheat. Here, we show that their influence also extends to spike and shoot architecture even impacts root development. The sequence diversity three the European winter wheat was analyzed by Oxford Nanopore amplicon-based multiplex sequencing molecular markers panel 194 cultivars representing breeding progress over past 5 decades. A strong, consistent association with an average 8%...

10.1007/s00122-024-04634-9 article EN cc-by Theoretical and Applied Genetics 2024-05-10

The incorporation of root traits into elite germplasm is typically a slow process. Thus, innovative approaches are required to accelerate research and pre-breeding programs targeting improve yield stability in different environments soil types. Marker-assisted selection (MAS) can help speed up the process by selecting key genes or quantitative trait loci (QTL) associated with traits. However, this approach limited due complex genetic control number well-characterised large effect QTL....

10.1186/s13007-021-00834-2 article EN cc-by Plant Methods 2022-01-10

The gene VERNALIZATION1 ( VRN1 ) is a key controller of vernalization requirement in wheat. genome hexaploid wheat Triticum aestivum harbors three homoeologous loci on chromosomes 5A, 5B, and 5D. Structural sequence variants including small large deletions insertions single nucleotide polymorphisms (SNPs) the genes not only play an important role control requirement, but also have been reported to be associated with other yield related traits Here we used single-molecule sequencing barcoded...

10.3389/fpls.2022.942461 article EN cc-by Frontiers in Plant Science 2022-07-15

In a cross between two homozygous Brassica napus plants of synthetic and natural origin, we demonstrate that novel structural genome variants from the parent cause immediate diversification among F1 offspring. Long read sequencing in twelve sister revealed five large-scale rearrangements where both parents carried different alleles but heterozygous genomes were not identical heterozygotes as expected. Such spontaneous part homoeologous exchanges or segmental deletions identified different,...

10.3389/fpls.2022.1057953 article EN cc-by Frontiers in Plant Science 2022-11-18

Abstract Seminal root angle (SRA) is an important architectural trait associated with drought adaptation in cereal crops. To date, all attempts to dissect the genetic architecture of SRA durum wheat ( Triticum Desf.) have used large association panels or structured mapping populations. Identifying changes allele frequency generated by selection provides alternative approach that can increase power and precision QTL detection. This study aimed map quantitative loci (QTL) for genotyping lines...

10.1002/tpg2.20490 article EN cc-by-nc-nd The Plant Genome 2024-07-23

Abstract In a cross between two homozygous Brassica napus plants of synthetic and natural origin, we demonstrate that novel structural genome variants from the parent cause immediate diversification among F1 offspring. Long read sequencing in twelve sister revealed five large-scale rearrangements where both parents carried different alleles but heterozygous genomes were not identical heterozygotes as expected. Such spontaneous part homoeologous exchanges or segmental deletions identified...

10.1101/2022.06.27.497715 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2022-06-29
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