A5074950183- Cancer Genomics and Diagnostics
- Platelet Disorders and Treatments
- Multiple Myeloma Research and Treatments
- Chronic Lymphocytic Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Hemophilia Treatment and Research
- Acute Myeloid Leukemia Research
- Protein Degradation and Inhibitors
- Cancer Immunotherapy and Biomarkers
- Autoimmune Bullous Skin Diseases
- Lung Cancer Treatments and Mutations
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Radiomics and Machine Learning in Medical Imaging
- RNA Interference and Gene Delivery
- Bioinformatics and Genomic Networks
- Alzheimer's disease research and treatments
- HIV/AIDS drug development and treatment
- Venous Thromboembolism Diagnosis and Management
- Histone Deacetylase Inhibitors Research
- Molecular Biology Techniques and Applications
- Blood groups and transfusion
- RNA modifications and cancer
- PI3K/AKT/mTOR signaling in cancer
- Blood properties and coagulation
North China University of Science and Technology Affiliated Hospital
2018-2025
North China University of Science and Technology
2015-2024
Intermolecular (United States)
2013-2018
Nanyang Institute of Technology
2014
Dana-Farber Cancer Institute
2010-2013
SunEdison (United States)
2013
Center for Excellence in Molecular Cell Science
2012
Shanghai Institutes for Biological Sciences
2012
Chinese Academy of Sciences
2012
Tongji University
2012
Dysregulation of autophagy, a cellular catabolic mechanism essential for degradation misfolded proteins, has been implicated in multiple neurodegenerative diseases. However, the mechanisms that lead to autophagy dysfunction are still not clear. Based on results genome-wide screen, we show reactive oxygen species (ROS) serve as common mediators upstream activation type III PI3 kinase, which is critical initiation autophagy. Furthermore, ROS play an function induction kinase and response...
Background Immune activation is a strong predictor of disease progression in HIV infection. Combinatorial plasma biomarker signatures that represent surrogate markers immune both viremic and aviremic patients on combination antiretroviral therapy (cART) have not been defined. Here, we identify inflammatory signature distinguishes between cART healthy controls examine relationships this to progression. Methods Multiplex profiling ELISA were used detect 15 cytokines/chemokines, soluble IL-2R...
We describe here a novel method for integrating gene and miRNA expression profiles in cancer using feed-forward loops (FFLs) consisting of transcription factors (TFs), miRNAs their common target genes. The dChip-GemiNI (Gene Network-based Integration) statistically ranks computationally predicted FFLs by explanatory power to account differential between two biological conditions such as normal cancer. GemiNI integrates not only data but also derived information about TF–target miRNA–mRNA...
Alzheimer's disease (AD) is a neurodegenerative disor-der and the most common cause of dementia in elderly people. Nu-merous studies have focused on dysregulated genes AD, but pathogenesis still unknown. In this study, we explored critical hippocampal pathways that might potentially be involved AD. Four transcriptome datasets for hip-pocampus patients with AD were downloaded from ArrayExpress, gene signature was identified by integrated analysis mul-tiple transcriptomes using novel...
Background & Objective Genome-wide profiles of tumors obtained using functional genomics platforms are being deposited to the public repositories at an astronomical scale, as a result focused efforts by individual laboratories and large projects such Cancer Genome Atlas (TCGA) International Consortium. Consequently, there is urgent need for reliable tools that integrate interpret these data in light current knowledge disseminate results biomedical researchers user-friendly manner. We have...
<title>Abstract</title> Building on promising phase 1 pilot results demonstrating safety and efficacy of BBM-H901 (AAV-mediated FIX Padua gene therapy) in restoring factor IX activity (FIX:C) among 10 patients with haemophilia B, we conducted current studies to verify its larger Chinese B patients. A multicentre, single-arm, open-label study (phase 1/2: dose-escalation, n = 6; 3: fixed-dose, 26) a long- term study(n 10) were conducted. Eligible participants received 5×10¹² vg/kg...
Summary Primary immune thrombocytopenia (ITP) is a haemorrhagic disorder with complex pathogenesis, wherein autoreactive B‐cell‐mediated platelet destruction plays crucial role. Bruton's tyrosine kinase (BTK) widely expressed and essential for cells. Several BTK inhibitors have been used clinically to treat haematological malignancies, while few studies are focusing on the regulatory role of in ITP. This study aims explore feasibility underlying mechanisms novel inhibitor orelabrutinib...
A novel, highly purified 10% intravenous immunoglobulin (IVIG) formulation was evaluated for both therapeutic efficacy and safety profile in adult patients diagnosed with persistent or chronic primary immune thrombocytopenia (ITP). This phase III, multicenter, open-label, single-arm clinical trial enrolled Chinese ITP presenting baseline platelet counts below 30 × 109/L. Participants received administration of IVIG at a standardized dosage 1 g/kg/day two consecutive days. The endpoint...
Abstract Background The clinical management of chronic myeloid leukemia (CML) patients requires the identification type BCR::ABL1 transcript at diagnosis and monitoring its expression potential tyrosine kinase inhibitor (TKI) resistance mutations during treatment. Detection resistant mutation type-specific amplification from RNA. Methods In this study, a custom RNA-based next-generation sequencing (NGS) assay (Dup-Seq BCR::ABL1) that enables (a) (b) detection common atypical types was...
Sphingosine kinase 1 (SphK1) is an oncogenic enzyme promoting transformation, proliferation, and angiogenesis of a number human tumors. However, its effect on hepatocellular carcinoma (HCC) behavior has not been fully clarified. The purpose this study was to determine the correlation between HCC SphK1, evaluate SphK1 inhibitor N,N-dimethylsphingosine (DMS) in HCC. expression measured tissue samples from 76 paired adjacent noncancerous liver tissues (NT) by immunohistochemistry, quantitative...
Frail elderly patients with newly diagnosed multiple myeloma (NDMM) have inferior survival and less benefit from high-dose therapies. This prospective study aimed to investigate the efficacy, safety, quality of life (QoL) induction treatment ixazomib/lenalidomide/dexamethasone (IRd) ixazomib/pegylated liposomal doxorubicin/dexamethasone (IDd) followed by ixazomib/dexamethasone (Id) maintenance therapy in frail, NDMM.
Abstract Introduction: The Oncomine™ Focus Assay (OFA) is aimed to simultaneously detect and report hotspot mutations, Copy Number Variants (CNVs) gene fusions in 52 oncogenes clinically relevant solid tumors. With minimal DNA/RNA input, OFA employs AmpliSeq™ library preparation chemistry enrich target regions for Ion Torrent™ Next Generation Sequencing. are annotated actionability by Oncomine® Knowledgebase. Here we an analytical validation of OFA. Methods: DNA RNA, extracted from the FFPE...
Abstract Introduction: The Oncomine® Cancer Panel (OCP) is designed to simultaneously detect and report hotspot mutations, Copy Number Variants (CNVs) gene fusions in 143 genes with minimal DNA/RNA sample input. OCP utilizes the AmpliSeq™ library preparation chemistry for Ion Torrent™ Next Generation Sequencing (NGS) platform, combination data annotations by Knowledgebase. Here we analytical validation of OCP. Methods: DNA RNA, extracted from FFPE processed GM12878, was used as negative...
Abstract The clinical management of CML and Ph+ ALL patients requires the identification BCR::ABL1 transcript variants drug resistance mutations. Currently these assessments need to be performed using separate assays resulting in higher sample requirements, longer turnaround times costs. In this study a custom NGS assay (Dup-Seq BCR-ABL1) was developed validated that enables co-detection (common atypical) design covers BCR exon 1 ABL1 10 employs duplicate PCR amplification BCR-ABL1 from...
Abstract Comprehensive genomic profiling (CGP) of tumor sample using large targeted NGS panels (e.g. MSK-IMPACT) has been shown to allow the detection biomarkers from hundreds cancer relevant genes involved in and immunotherapy. Illumina TruSight Oncology 500 (TSO500), a commercial CGP assay, allows concurrent analysis DNA RNA simultaneously detect multiple types variants across 523 genes. The assay is intended single nucleotide (SNV), insertions deletions (indel), multinucleotide (MNV),...