A5064011318- Insect and Pesticide Research
- Probiotics and Fermented Foods
- Plant and animal studies
- Genomics and Phylogenetic Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Vector-borne infectious diseases
- Antibiotic Resistance in Bacteria
- Insect and Arachnid Ecology and Behavior
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Animal Nutrition and Physiology
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Antibiotic Use and Resistance
- Cancer-related gene regulation
- Health and Medical Research Impacts
- Microbial infections and disease research
- Innovations in Medical Education
- Antimicrobial Peptides and Activities
- Viral Infections and Vectors
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Virus Infections Studies
- Ethics in medical practice
- Bartonella species infections research
- Therapeutic Uses of Natural Elements
University of Veterinary Medicine
2018-2025
Semmelweis University
2023-2025
Institute of Molecular Life Sciences
2023
HUN-REN Research Centre for Natural Sciences
2023
University of Leicester
2023
Government of Western Australia Department of Health
2023
Public Health England
2023
Hypomethylation of long interspersed nuclear element 1 (LINE-1) is characteristic various cancer types, including colorectal (CRC). Malfunction several factors or alteration methyl-donor molecules' (folic acid and S-adenosylmethionine) availability can contribute to DNA methylation changes. Detection epigenetic alterations in liquid biopsies assist the early recognition CRC. Following investigations a Hungarian colon tissue sample set, our goal was examine LINE-1 blood samples along...
BackgroundAntimicrobial resistance (AMR) is caused by AMR determinants, mainly genes (ARGs) in the bacterial genome. Bacteriophages, integrative mobile genetic elements (iMGEs) or plasmids can allow ARGs to be exchanged among bacteria horizontal gene transfer (HGT). Bacteria, including with ARGs, found food. Thus, it conceivable that gastrointestinal tract, from gut flora could take up food.AimThe study objective was gain insight into ARG set carried commonly used probiotic may enter human...
Neuropathy is a serious and frequent complication of type 2 diabetes (T2DM). This study was carried out to search for genetic factors associated with the development diabetic neuropathy by whole exome sequencing. For this study, 24 patients long-term without underwent detailed neurological assessment Cardiovascular autonomic function evaluated cardiovascular reflex tests. Heart rate variability measured triangle index. Sensory nerve estimated Neurometer Medoc devices. Neuropathic symptoms...
Abstract In Hungary, as part of a nationwide, climatically balanced survey for next-generation sequencing-based study the honey bee ( Apis mellifera ) gut microbiome, repeated sampling was carried out during production season (March and May 2019). Among other findings, presence filamentous virus Am FV) detected in all samples, some at very high levels. FV-derived reads were more abundant March samples than samples. March, higher abundance FV-originated identified collected from warmer areas...
As pollinators and producers of numerous human-consumed products, honey bees have great ecological, economic health importance. The composition their bacteriota, for which the available knowledge is limited, essential body’s functioning. Based on our survey, we performed a metagenomic analysis samples collected by repeated sampling. We used geolocations that represent climatic types study area over two nutritionally extreme periods (March May) collection season. Regarding bacteriome...
The diagnosis of porcine circovirus 2 (PCV-2) systemic disease is based on clinical signs, microscopic lesions, and semiquantitative PCV-2 assessment by in situ visualization immunohistochemistry (IHC) or hybridization labeling within lymphoid tissue lesions. We aimed to digitally quantify IHC-labeled PCV-2-infected cells lymph nodes with natural ( n = 53) compare these quantitative results the subjective manual method, also viral DNA copy numbers corresponding formalin-fixed,...
Introduction One of the greatest challenges our time is antimicrobial resistance, which could become leading cause death globally within a few decades. In context Health, it in common interest to mitigate global spread resistance by seeking alternative solutions, alongside appropriate drug selection and responsible use. Probiotics offer potential avenue reduce antibiotic usage; however, there scarcity research that examines commercial products terms carrying genes (ARGs) involved development...
The beneficial effects of balneotherapy have been proven by numerous clinical studies on locomotor disorders. To date, there is only scant data changes in the microbiome system skin during balneotherapy. aim this study was to compare thermal water and tap skin's healthy volunteers. 30 female volunteers participated study. experimental group (of 15 women) spent 30-min 10 times, Gabriella Spring's baths (i.e., mineral containing sodium hydrogen carbonate).The controlled (15 had same, but...
Abstract To date, single-nucleotide polymorphisms (SNPs) have been the most intensively investigated class of in genome wide associations studies (GWAS), however, other classes such as insertion-deletion or multiple nucleotide length polymorphism (MNLPs) may also confer disease risk. Multiple reports shown that 5p15.33 prostate cancer risk region is a particularly strong expression quantitative trait locus (eQTL) for Iroquois Homeobox 4 ( IRX4) transcripts. Here, we demonstrate using...
The authors aimed to investigate eight strains of Escherichia coli (E. coli) from Hungarian layer flocks for antimicrobial resistance genes (ARG), using metagenomic methods. were isolated cloacal swabs healthy adult layers. This study employed shotgun sequencing-based genetic and bioinformatic analysis along with determining phenotypic minimum inhibitory concentrations. A total 59 ARGs identified in the E. isolates, carrying against 15 groups antibiotics. Among these, 28 as transferable....
Genetic alterations are well known to be related the pathogenesis and prognosis of papillary thyroid carcinoma (PTC). Some miRNA expression dysregulations have previously been described in context cancer development including carcinoma. In our study, we performed original molecular diagnostics on tissue samples own patients. We aimed identify all dysregulated miRNAs potential association with PTC via sequencing much higher numbers control-matched analyzing a wider variety types than previous...
Methicillin-resistant Staphylococcus aureus bearing the mecC gene (mecC-MRSA) has been reported from animals and humans in recent years. This study describes first mecC-MRSA isolates of human equine origin Hungary (two horses one a veterinarian, who treated infected horses, but was asymptomatic). MRSA were identified by cultivation PCR detection species-specific spa mecA/mecC methicillin resistance genes. The characterized antibiotic susceptibility testing, MLST, spa, SCCmec typing, PFGE...
Livestock-Associated Methicillin-ResistantStaphylococcus aureus (LA-MRSA) strains of clonal complex (CC) 398 are widely disseminated in pigs and considered emerging pathogens human medicine. To investigate the prevalence, genetic characteristics, zoonotic potential pathogen pig production settings, dust samples were collected from 40 operations Hungary, along with nasal swabs attending veterinarians other swine professionals (n = 27) 2019. MRSA isolates further characterized by performing...
Abstract Tick-borne infections, including those of bacterial origin, are significant public health issues. Antimicrobial resistance (AMR), which is one the most pressing challenges our time, driven by specific genetic determinants, primarily antimicrobial genes (ARGs) bacteria. In work, we investigated occurrence ARGs in genomes tick-borne species that can cause human infections. For this purpose, processed short/long reads 1550 isolates genera Anaplasma (n = 20), Bartonella 131), Borrelia...
Climate-sensitive disease vectors, such as ticks, respond to the environment with changes in their microbiome. These can affect emergence or re-emergence of various vector-borne pathogens, causative agent Lyme borreliosis (LB) tick-borne encephalitis. This aspect is particularly emphasized light climate change. The climatically representative assessment microbiome differences developmental stages most common Central European tick species, Ixodes ricinus, deepens our understanding potential...
The liver with resident tissue macrophages is the site of vivid innate immunity, activated also by pathogen-associated molecular patterns (PAMPs) leaking through intestinal barrier. As gut-derived inflammatory diseases are outstanding importance in broiler chickens, present study aimed to establish a proper hepatic model comparing action different PAMPs from poultry pathogens on chicken 2D and 3D primary hepatocyte—non-parenchymal cell co-cultures, latter newly developed magnetic bioprinting...
As the prevalence of antimicrobial resistance genes is increasing in microbes, we are facing return preantibiotic era. Consecutively, number studies concerning antibiotic and its spread environment rapidly growing. Next generation sequencing technologies widespread used many areas biological research no exception. For rapid annotation whole genome metagenomic results considering resistance, several tools data resources were developed. These databases, however can differ fundamentally type...
Single-cell transcriptomics are powerful tools to define neuronal cell types based on co-expressed gene clusters. Limited RNA input in these technologies necessarily compromises transcriptome coverage and accuracy of differential expression analysis. We propose that bulk RNA-Seq pools defined by spatial position offers an alternative strategy overcome technical limitations. report a laser-capture microdissection (LCM)-Seq method which allows deep profiling fluorescently tagged neuron...
Raw milk and dairy products can serve as potential vectors for transmissible bacterial, viral protozoal diseases, alongside harboring antimicrobial-resistance genes. This study monitors the changes in gene pool raw cheese, from farm to consumer, utilizing next-generation sequencing. Five parallel sampling runs were conducted assess resistance pool, well phage or plasmid carriage mobility. In terms of taxonomic composition, Firmicutes phylum made up 41%, while Proteobacteria accounted 58%....
Probiotics are widely used in broiler chickens to support the gut microbiome, health, and reduce amount of antibiotics used. Despite their benefits, there is concern over ability carry spread antimicrobial resistance genes (ARGs), posing a significant public health risk. This study utilized next-generation sequencing investigate ARGs probiotics approved for poultry, focusing on potential be transferred via mobile genetic elements such as plasmids phages. We examined microbiome resistome...