A5016935487- Insect and Pesticide Research
- Hippo pathway signaling and YAP/TAZ
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Fibroblast Growth Factor Research
- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- Wnt/β-catenin signaling in development and cancer
- Bee Products Chemical Analysis
- Invertebrate Immune Response Mechanisms
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Animal Genetics and Reproduction
- Protease and Inhibitor Mechanisms
- Signaling Pathways in Disease
University of Maryland, College Park
2013-2022
Center for Biologics Evaluation and Research
2021
Honey bee health is mainly affected by Varroa destructor, viruses, Nosema spp., pesticide residues and poor nutrition. Interactions between these proposed factors may be responsible for the colony losses reported worldwide in recent years. In present study, effects of a honey virus, Israeli acute paralysis virus (IAPV), on foraging behaviors homing ability European bees (Apis mellifera L.) were investigated based proboscis extension response (PER) assays radio frequency identification (RFID)...
Gradients of signaling proteins are essential for inducing tissue morphogenesis. However, mechanisms gradient formation remain controversial. Here we characterized the distribution fluorescently-tagged proteins, FGF and FGFR, expressed at physiological levels from genomic knock-in alleles in Drosophila. produced larval wing imaginal-disc moves to air-sac-primordium (ASP) through FGFR-containing cytonemes that extend ASP contact wing-disc source. The number FGF-receiving extended by cells...
The aim of this study was to improve cage systems for maintaining adult honey bee (Apis mellifera L.) workers under in vitro laboratory conditions. To achieve goal, we experimentally evaluated the impact different cages, developed by scientists international research network COLOSS (Prevention COlony LOSSes), on physiology and survival bees. We identified three cages that promoted good bees from exhibited greater had relatively lower titers deformed wing virus, suggesting virus is a...
Ovarian activity not only influences fertility, but is also involved with the regulation of division labour between reproductive and behavioural castes female honey bees. In order to identify candidate genes associated ovarian activity, we compared gene expression patterns inactivated activated ovaries queens workers by means high-throughput RNA-sequencing technology. A total 1615 differentially expressed (DEGs) was detected virgin mated queens, more than 5300 DEGs were worker ovaries....
Nosema ceranae Fries et al., 1996, a microsporidian parasite recently transferred from Asian honey bees Apis cerana F., 1793, to European mellifera L., 1758, has been suspected as one of the major culprits worldwide bee colony losses. Spore load is commonly used criterion describe intensity infection. In this study, by providing Nosema-infected with sterilized pollen, we confirmed that pollen feeding increased spore loads several times either in presence or absence queen. By changing amount...
Abstract How signaling proteins generate a multitude of information to organize tissue patterns is critical understanding morphogenesis. In Drosophila , FGF produced in wing-disc cells regulates the development disc-associated air-sac-primordium (ASP). Here, we show that Glycosylphosphatidylinositol-anchored producing cell surface and this modification both inhibits free secretion promotes target-specific cytoneme contacts contact-dependent release. FGF-source ASP extend cytonemes present...
How morphogenetic signals are prepared for intercellular dispersal and signaling is fundamental to the understanding of tissue morphogenesis. We discovered an intracellular mechanism that prepares Drosophila melanogaster FGF Branchless (Bnl) cytoneme-mediated during development larval Air-Sac-Primordium (ASP). Wing-disc cells express Bnl as a proprotein cleaved by Furin1 in Golgi. Truncated sorts asymmetrically basal surface, where it received cytonemes extend from recipient ASP cells....
Binary transcription systems are powerful genetic tools widely used for visualizing and manipulating cell fate gene expression in specific groups of cells or tissues model organisms. These contain two components as separate transgenic lines. A driver line expresses a transcriptional activator under the control tissue-specific promoters/enhancers, reporter/effector harbors target placed downstream to binding site activator. Animals harboring both induce transactivation expression. Precise...
ABSTRACT How signaling proteins generate a multitude of information to organize tissue patterns is critical understanding morphogenesis. In Drosophila , FGF produced in wing-disc cells regulates the development disc-associated air-sac-primordium (ASP). Here, we show that Glycosylphosphatidylinositol-anchored producing cell surface and this modification both inhibits free secretion activates target-specific bidirectional FGF-FGFR through cytonemes. FGF-source recipient ASP extend cytonemes...
Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways.Classical genetic approaches in vitro biochemical or cell biological assays identified precursor forms most growth factor proteins processed by Furin.To quantitatively assess the potential role cleaving modulating intercellular dispersion Drosophila signaling protein, we developed simple assay combining...