A5032694129- Alzheimer's disease research and treatments
- Protein Structure and Dynamics
- Rheumatoid Arthritis Research and Therapies
- Monoclonal and Polyclonal Antibodies Research
- Cardiac pacing and defibrillation studies
- Enzyme Structure and Function
- Neuroinflammation and Neurodegeneration Mechanisms
- Hepatitis B Virus Studies
- Protein Interaction Studies and Fluorescence Analysis
- Crystallization and Solubility Studies
- Cardiac Arrhythmias and Treatments
- Neuroscience and Neuropharmacology Research
- Neurological Disease Mechanisms and Treatments
- Atrial Fibrillation Management and Outcomes
- Computational Drug Discovery Methods
- Systemic Lupus Erythematosus Research
- Adrenal Hormones and Disorders
- Autoimmune and Inflammatory Disorders Research
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- Immunodeficiency and Autoimmune Disorders
- Cell Adhesion Molecules Research
- Cardiovascular Function and Risk Factors
- Supramolecular Self-Assembly in Materials
- Cellular transport and secretion
HM Hospitales
2022
Janssen (Netherlands)
2018
Johnson & Johnson (Netherlands)
2018
Johnson & Johnson (Sweden)
2018
Janssen (Belgium)
2018
Universidade do Porto
2011-2017
Center for Rheumatology
2013
Instituto de Biologia Molecular e Celular
2010-2011
The aggregation of amyloid-β peptide (Aβ) has been linked to the formation neuritic plaques, which are pathological hallmarks Alzheimer's disease. We synthesized peptides containing fluorinated amino acids and studied their effect on Aβ aggregation. were based sequence LVFFD, in valine was substituted by either 4,4,4-trifluorovaline or 4-fluoroproline, phenylalanine at position 3 replaced 3,4,5-trifluorophenylalanine. Our results demonstrate that fluorination hydrophobic residue is effective...
This work shows promising applications of ultrasound in promoting protein crystallization, which is important for structure determination by X-ray crystallography. It was observed that can be used as a nucleation promoter it decreases the energy barrier crystal formation. Crystallization experiments on egg-white lysozyme were carried out with and without ultrasonic irradiation using commercial crystallization plates placed temperature-controlled water baths. The nucleation-promoting effect...
Misfolding and aggregation of tau protein are closely associated with the onset progression Alzheimer's Disease (AD). By interrogating IgG+ memory B cells from asymptomatic donors peptides, we have identified two somatically mutated VH5–51/VL4–1 antibodies. One these, CBTAU-27.1, binds to motif in R3 repeat domain blocks into paired helical filaments (PHFs) by sequestering monomeric tau. The other, CBTAU-28.1, N-terminal insert region inhibits spreading seeds mediates uptake aggregates...
Aggregation of tau protein and spreading aggregates are pivotal pathological processes in a range neurological disorders. Accumulating evidence suggests that immunotherapy targeting may be viable therapeutic strategy. We have previously described the isolation antibody CBTAU-22.1 from memory B-cell repertoire healthy human donors. was shown to specifically bind disease-associated phosphorylated epitope C-terminus (Ser422) able inhibit P301S spinal cord lysates vitro, albeit with limited...
The methodology adopted by Michaelis and Menten in 1913 is still routinely used to characterize the catalytic power selectivity of enzymes. These kinetic measurements must be performed soon after purified enzyme mixed with a large excess substrate. Other time scales solution compositions are no less physiologically relevant, but fall outside range applicability classical formalism. Here we show that complete picture an enzyme's mode function critically obscured limited scope conventional...
Aggregation of tau protein and formation paired helical filaments is a hallmark Alzheimer's disease other tauopathies. Compared to proteins associated with neurodegenerative diseases, the reported in vitro aggregation kinetics for are less consistent presenting relatively high variability. Here we describe development an assay that mimics expected steps misfolding vivo. The uses longest isoform (huTau441) which contains both N-terminal acidic inserts as well four microtubule binding domains...
The study of drug candidates for the treatment amyloidosis and neurodegenerative diseases frequently involves in vitro measurements amyloid fibril formation. Macromolecular crowding off-pathway aggregation (OPA) are, by different reasons, two important phenomena affecting scalability inhibitors their successful application vivo. On one hand, cellular milieu is crowded with macromolecules that drastically increase effective (thermodynamic) concentration amyloidogenic protein. other...
Small temperature oscillations (TOs) are shown to increase the success rates of protein crystallization experiments by enhancing nucleation and inducing formation larger crystals. This effect was initially found when we accidentally used a defective incubator it is now confirmed for egg-white lysozyme at 14 20 °C, also glucose isomerase both under experimental conditions close zone proteins' phase diagram. Measurements times constant precipitant concentrations show that promoting due...
Alzheimer's disease (AD) is a progressive neurodegenerative condition in which aggregated tau and amyloid proteins accumulate the brain causing neuronal dysfunction eventually leads to cognitive decline. Hyperphosphorylated aggregates neuron are believed cause most of pathology associated with AD. These assumed be released into extracellular compartment taken up by adjacent healthy neurons where they induce further aggregation. This "prion-like" spreading can interrupted antibodies capable...
Purpose: Pacing at a fixed output have been used as guide to detect gaps after pulmonary vein isolation (PVI). This approach assumes that tissue capture during pacing is good surrogate for contact force (CF). There no information about the relationship between CF and threshold. The aim of our study analyze threshold in left atrium (LA).
<h3>Background</h3> In recent years the different drug treatments in rheumatic patients can lead to situations of immunosuppression associated with an increased risk infection by immunization. <h3>Objectives</h3> To present preliminary results vaccination against hepatitis B double-dose schedule and its effectiveness these patients. following protocol Hospital Universitario 12 de Octubre. <h3>Methods</h3> Of 532 adult referred Department Rheumatology at Immunization Unit Preventive Medicine...
<h3>Background</h3> The different drug treatments in rheumatic patients can lead to situations of immunosuppression and predispose infection by immunization. <h3>Objectives</h3> To present preliminary results antibody response immunisation with a double-doses recombinant hepatitis B vaccine its effectiveness these patients. <h3>Methods</h3> Of the 532 adult referred Department Rheumatology at Immunization Unit Preventive Medicine this hospital assess their vaccination status from beginning...
ABSTRACT Misfolding and aggregation of tau protein are closely associated with the onset progression Alzheimer’s Disease (AD). By interrogating IgG + memory B cells from asymptomatic donors peptides, we have identified two somatically mutated V H 5-51/V L 4-1 antibodies. One these, CBTAU-27.1, binds to motif in R3 repeat domain blocks into paired helical filaments (PHFs) by sequestering monomeric tau. The other, CBTAU-28.1, N-terminal insert region inhibits spreading seeds mediates uptake...
Alzheimer's disease (AD) is a progressive neurodegenerative condition in which aggregated tau and amyloid proteins accumulate the brain causing neuronal dysfunction eventually leads to cognitive decline. Hyperphosphorylated aggregates neuron are believed cause most of pathology associated with AD. These assumed be released into extracellular compartment taken up by adjacent healthy neurons where they induce further aggregation. This "prion-like" spreading can interrupted antibodies capable...