A5038187351- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
EpiCypher (United States)
2022-2024
Histone post-translational modifications (PTMs) play a critical role in chromatin regulation. It has been proposed that these PTMs form localized 'codes' are read by specialized regions (reader domains) chromatin-associated proteins (CAPs) to regulate downstream function. Substantial effort made define [CAP: histone PTM] specificities, and thus decipher the code guide epigenetic therapies. However, this largely done using reductive approach of isolated reader domains peptides, which cannot...
In nucleosomes, histone N-terminal tails exist in dynamic equilibrium between free/accessible and collapsed/DNA-bound states. The latter state is expected to impact N-termini availability the epigenetic machinery. Notably, H3 tail acetylation (e.g. K9ac, K14ac, K18ac) linked increased H3K4me3 engagement by BPTF PHD finger, but it unknown if this mechanism has a broader extension. Here, we show that promotes nucleosomal accessibility other H3K4 methyl readers, importantly, extends writers,...
The role of histone post-translational modifications (PTMs) in chromatin structure and genome function has been the subject intense debate for more than 60 years. Though complex, discourse can be summarized two distinct — deceptively simple questions: What is PTMs? And how should they studied? Decades research show these queries are intricately linked far from straightforward. Here we provide a historical perspective, highlighting arrival new technologies shaped discovery insight. Despite...
The precise regulation of chromatin composition is critical to gene expression and cellular identity, thus a key component in development disease. Robust assays study features, including histone post-translational modifications (PTMs) chromatin-associated proteins (e.g., transcription factors or PTM readers), are essential understand their function identify novel therapeutic strategies. To this end, Cleavage Under Targets Release Using Nuclease (CUT&RUN) has emerged as powerful tool for...
ABSTRACT In nucleosomes, histone N-terminal tails exist in dynamic equilibrium between free/accessible and collapsed/DNA-bound states. The latter state is expected to impact N-termini availability the epigenetic machinery. Notably, H3 tail acetylation ( e.g. , K9ac, K14ac, K18ac) linked increased H3K4me3 engagement by BPTF PHD finger, but it unknown if this mechanism has broader extension. Here we show that promotes nucleosomal accessibility other H3K4 methyl readers, importantly, extends...
Abstract Chromatin structure drives gene expression programs during immune cell development and in cancer, making it central to the advancement of precision medicine. Indeed, study chromatin regulation has provided valuable insight on effector differentiation function, anti-tumor responses, therapeutic resistance. Epigenomic features – such as histone post-translational modifications (PTMs) chromatin-associated proteins mark distinct genomic compartments (e.g., promoters, enhancers) regulate...
Abstract Histone post-translational modifications (PTMs) play a critical role in chromatin regulation. It has long been suspected that the poorly understood capability of ‘PTM-specific’ antibodies (i.e., their specificity and efficiency) is prime driver reproducibility crisis biomedical research. Here we confirm validity this concern as it applies to epigenomic mapping studies. Extensive spike-in panels PTM-defined DNA-barcoded nucleosome standards show >70% >500 commercial...