A5007775614- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Plant responses to water stress
- Lipid metabolism and biosynthesis
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Light effects on plants
- Heat shock proteins research
- Plant Stress Responses and Tolerance
- Genomic variations and chromosomal abnormalities
- Soybean genetics and cultivation
- Plant Parasitism and Resistance
- Animal Genetics and Reproduction
- Oral and gingival health research
- Enzyme Structure and Function
- Genomics and Rare Diseases
- Protein Structure and Dynamics
Nanjing Agricultural University
2019-2024
Shanghai Normal University
2018-2021
Wuhan University
2015
Greenwood Genetic Center
2012
Abstract Reporters have been widely used to visualize gene expression, protein localization, and other cellular activities, but the commonly reporters require special equipment, expensive chemicals, or invasive treatments. Here, we construct a new reporter RUBY that converts tyrosine vividly red betalain, which is clearly visible naked eyes without need of using equipment chemical We show can be noninvasively monitor expression in plants. Furthermore, an effective selection marker for...
The outer wall of pollen and spores, namely the exine, is composed sporopollenin, which highly resistant to chemical reagents enzymes. In this study, we demonstrated that phenylpropanoid pathway derivatives are essential components sporopollenin in seed plants. Spectral analyses showed autofluorescence Lilium Arabidopsis similar lignin. Thioacidolysis NMR from Cryptomeria further revealed plants contains derivatives, including p-hydroxybenzoate (p-BA), p-coumarate (p-CA), ferulate (FA),...
Abstract DNA methylation is an important epigenetic mark that regulates the expression of genes and transposons. RNA-directed (RdDM) main molecular pathway responsible for de novo in plants. Although mechanism RdDM has been well studied Arabidopsis (Arabidopsis thaliana), most mutations cause no remarkable developmental defects Arabidopsis. Here, we isolated cloned Five Elements Mountain 1 (FEM1), which encodes RNA-dependent RNA polymerase 2 (OsRDR2) rice (Oryza sativa). Mutation OsRDR2...
We present a case of 9-month-old Hispanic female with Kabuki syndrome some infrequent manifestations including single umbilical artery, butterfly vertebrae, small larynx, preauricular pit, microtia internal ear abnormalities, abnormal calcium metabolism, premature thelarche, neonatal/persistent hypoglycemia and eventration the diaphragm. She was found to have previously unreported nonsense <i>MLL2</i> mutation. This is first that includes all such findings occurring...
Summary Inositol polyphosphate kinase ( IPK 2) is a key component of inositol signaling. There are two highly homologous kinases A t 2α and 2β) in rabidopsis. Previous studies that overexpressed or reduced the expression At 2β revealed their roles auxiliary shoot branching, abiotic stress responses root growth. Here, we report 2 α β act redundantly during pollen development, tube guidance embryogenesis. Single knock‐out mutants atipk2 were indistinguishable from wild type, whereas double...
CRISPR/Cas9 gene editing technology has been very effective in genes many plant species including rice. Here we further improve the current both efficiency and time needed for isolation of transgene-free target gene-edited plants. We coupled cassette with a unit that activates anthocyanin biosynthesis, providing visible marker detecting presence transgenes. The anthocyanin-marker assisted CRISPR (AAC) enables us to identify transgenic events even at calli stage, select transformants elevated...
Abstract The sporopollenin polymer is a major component of the pollen exine. Fatty acid derivatives synthesized in tapetum are among precursors sporopollenin. Progress has been made to understand metabolism rice; however, underlying molecular mechanisms remain elusive. We found that OsTKPR2 and OsTKPR1 share similar expression pattern, their coding proteins have subcellular localization enzyme activities towards reduced tetraketide α-pyrone hydroxylated α-pyrone. Unexpectedly,...
Heat-shock protein of 90 kDa (Hsp90) is a key molecular chaperone involved in folding the synthesized and controlling quality. Conformational dynamics coupled to ATPase activity N-terminal domain essential for Hsp90's function. However, relevant process still largely unknown plant Hsp90s, especially those required embryogenesis which inextricably tied up with human survival. Here, AtHsp90.6, member Hsp90 family Arabidopsis , was firstly identified as embryogenesis. Thus we modelled AtHsp90.6...
Pollen coat lipids form an outer barrier to protect pollen itself and play essential roles in pollen-stigma interaction. However, the precise molecular mechanisms underlying production, deposition, regulation, function of during anther development remain largely elusive. In lipid metabolism, 3-ketoacyl-coenzyme A synthases (KCS) are involved fatty acid elongation or very-long-chain (VLCFA) synthesis. this study, we identified six members Arabidopsis KCS family expressed anther. Among them,...
Abstract Brassinosteroids (BRs) are a group of steroid hormones that play crucial roles in plant growth and development. Atypical bHLH transcription factors lack the basic region for DNA binding have been implicated BR signaling. However, underlying mechanisms atypical bHLHs regulation rice (Oryza sativa) signaling still largely unknown. Here, we describe systematic characterization INCREASED LEAF INCLINATION (ILI) subfamily rice. A total 8 members, ILI1 to ILI8, with substantial sequence...
Genetically elucidating functions of gene families often requires simultaneous disruption several family members to overcome redundancy. CRISPR/Cas9-based editing technology can simultaneously knockout multiple target genes (Armario Najera et al., 2019; Li 2021; Ma 2015), but it is laborious isolate null combinations lacking transgenes (Miao 2018; Zhao 2018). Removal necessary because the CRISPR components generate new mutations and off-target events in next generations (Zhang 2014, Genetic...
Atypical basic helix-loop-helix (bHLH) transcription factors, which lack the region for DNA binding, are important elements of brassinosteroid (BR) signaling. Recently, our systematic characterization rice (Oryza sativa) INCREASED LEAF INCLINATION (ILI) subfamily atypical bHLHs revealed their indispensable roles in BR-mediated growth and development. Here, we reported isolation two additional ILI-interacting bHLHs, ATBS1-INTERACTING FACTOR 1 (OsAIF1)/OsbHLH176 OsAIF2/OsbHLH178. Genetic...
Abstract DNA methylation is an important epigenetic mark that regulates the expression of genes and transposons. RNA-directed (RdDM) main molecular pathway responsible for de novo in plants. In Arabidopsis, however, mutations RdDM cause no visible developmental defects, which raising question biological significance plant development. Here, we isolated cloned Five Elements Mountain 1 ( FEM1 ), encodes RNA-dependent RNA polymerase. Mutation substantially decreased genome-wide CHH levels...
Pollen coat lipids form an outer barrier to protect pollen itself and play essential roles in pollen-stigma interaction. However, the precise molecular mechanisms underlying production, deposition, regulation function during anther development remain largely elusive. 3-ketoacyl-coenzyme A synthases (KCS) are involved fatty acid elongation or very long chain (VLCFA) synthesis. Here, we identified six members of Arabidopsis KCS family expressed anther. Of them, KCS7, KCS15 KCS21 express...