A5068596801- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- HER2/EGFR in Cancer Research
- Cell Adhesion Molecules Research
- Advanced Biosensing Techniques and Applications
- Cancer Treatment and Pharmacology
- Trace Elements in Health
- Radiopharmaceutical Chemistry and Applications
- Cancer Research and Treatments
- Virus-based gene therapy research
- Pesticide Exposure and Toxicity
- Cancer Cells and Metastasis
- Cassava research and cyanide
- Insect and Pesticide Research
- Sarcoma Diagnosis and Treatment
- Toxin Mechanisms and Immunotoxins
- Cannabis and Cannabinoid Research
- Neurological Disorders and Treatments
- Occupational exposure and asthma
- Pharmacological Receptor Mechanisms and Effects
- Caveolin-1 and cellular processes
- Proteoglycans and glycosaminoglycans research
- Immune cells in cancer
- Microbial Inactivation Methods
- Gastrointestinal Tumor Research and Treatment
Seagen (United States)
2007-2024
United States Army
2003-2008
Corixa Corporation
2006
United States Army Medical Research Institute of Infectious Diseases
2003
United States Department of the Army
2002
CECOM Software Engineering Center
2000
Armed Forces Institute of Pathology
1999
CD133/prominin-1 is a pentaspan transmembrane glycoprotein overexpressed in various solid tumours including colorectal and glioblastomas. CD133 was found here to be highly expressed >or=50% of pancreatic, gastric intrahepatic cholangiocarcinomas. Quantitative flow cytometric analysis showed that panel established hepatocellular, pancreatic cancer cell lines at levels higher than normal epithelial cells or bone marrow progenitor cells. A murine anti-human antibody (AC133) conjugated potent...
Ricin is a toxic lectin derived from the seed of Ricinus communis (castor plant). It lethal in small quantities when disseminated as an aerosol. We determined impact using two types exposure chambers and different particle sizes on deposition ricin aerosols mice. Initially, inhalation (whole-body [WB] or nose-only [NO]) were compared same size aerosol (1 micro m) to determine potential upon respiratory presented dose. then assessed role by with distinctly sized distributions. Selected organs...
CD70 is an ideal target for antibody-based therapies because of its aberrant high expression in renal carcinomas and non-Hodgkin lymphomas highly restricted normal tissues. The profiling has been limited the lack a CD70-specific reagent that works formalin-fixed paraffin-embedded (FFPE) We generated murine monoclonal antibodies (mAbs) specific validated their specificity by western blot analysis developed protocol immunohistochemistry on FFPE CD70+ tumour cell lines were used testing...
CD70 (CD27L) is a member of the tumor necrosis factor family aberrantly expressed on number hematologic malignancies and some carcinomas. expression malignant cells coupled with its highly restricted normal makes an attractive target for monoclonal antibody (mAb)-based therapies. We developed humanized anti-CD70 antibody, SGN-70, herein describe antitumor activities this mAb.CD70 primary tumors was evaluated by immunohistochemical staining Hodgkin lymphoma, non-Hodgkin multiple myeloma,...
L523S is an immunogenic lung cancer antigen that has demonstrated preclinical safety when the gene injected intramuscularly as expressive plasmid (pVAX/L523S) and delivered following incorporation into E1B-deleted adenovirus (Ad/L523S). We performed a phase I clinical trial in 13 stage IB, IIA, IIB non-small-cell patients. pVAX/L523S (8 mg on days 0 14 all cohorts) Ad/L523S (1, 20, 400 × 109 vp 28 56, cohorts 1, 2, 3, respectively) were administered to 3 patients each of three cohorts. No...
Abstract Here, we report the development of an antibody–drug conjugate, ASG-5ME, which targets solute carrier receptor SLC44A4. SLC44A4 is a member family putative choline transporters that show to be markedly upregulated in variety epithelial tumors, most notably prostate and pancreatic cancer. normally expressed on apical surface secretory cells, but cancer expression not restricted luminal advanced undifferentiated tumors. ASG-5ME consists human IgG2 anti-SLC44A4 antibody conjugated...
Phosgene (CG) is a highly irritant gas widely used industrially as chemical intermediate for the production of dyes, pesticides, and plastics, can cause life-threatening pulmonary edema within 24 hours exposure. This study was designed to investigate acute changes in lung tissue histopathology selected bronchoalveolar lavage fluid (BALF) factors over time determine early diagnostic indicators Three groups 40 male mice each were exposed 32 mg/m3 (8 ppm) CG 20 minutes, 3 control filtered room...
Abstract Nonclinical safety and pharmacokinetic data for monomethyl auristatin E (MMAE) 14 vedotin antibody–drug conjugates (ADC) were evaluated to determine patterns of toxicity, consistency results, species differences between rats monkeys. Most nonclinical toxicities antigen-independent, common across ADCs, included hematologic, lymphoid, reproductive toxicity related MMAE pharmacology. Hematologic was the dose-limiting (DLT) or predominant majority ADCs in both species. Tissue expression...
An animal dosing model and related maximum tolerated dose (MTD) were developed for repeated exposures in guinea pigs to three organophosphorus chemical warfare nerve agents (CWNA). Male animals injected subcutaneously with sarin (GB), soman (GD) or VX once a day (Monday through Friday) 2-, 4-, 13-weeks. initial 13-week study each CWNA employed doses of vehicle (normal saline), 0.2x, 0.4x, 0.6x, 0.8x the previously established acute LD(50). A 2-week 4-week exposure also performed agent at...
An 18-year-old gelding Thoroughbred was referred to Vir-ginia–Maryland Regional College of Veterinary Medicinefor evaluation anorexia, pyrexia, chronic weight loss, anda distended abdomen. Three years previously, this horse wasdiagnosed with acute septic hepatocholangitis based onphysical, bacteriologic, biochemical, and histopathologicfindings.Streptococcus viridans isolated from the liverbiopsy samples. Treatment included intravenous fluids, B vi-tamins, antibiotics. The recovered had...
Abstract AGS-5 is a novel transmembrane antigen discovered by Agensys using transcriptional profiling that highly expressed in variety of epithelial tumors. A fully human IgG2k monoclonal antibody binds to with high affinity was conjugated the anti-microtubulin drug, monomethyl auristatin E (MMAE), via conditionally labile valine-citrulline (vc) maleimidocaproyl linker yield an average drug: ratio 3.7. Following cell surface binding, ASG-5ME internalized and trafficked through endocytic...
A 6-year-old spayed female Domestic Shorthair cat presented with a 1 to 2-month history of blindness and altered behavior. At necropsy, 1-cm-diameter, firm white mass was found arising from the subependymal region right lateral ventricular wall that protruded into partially filled lumen. Histologically, there well-demarcated, expansile paraventricular neoplasm composed moderately pleomorphic cells within richly fibrillar matrix arranged in interlacing streams perivascular pseudorosette-like...
Ricin is a toxic lectin derived from the seed of Ricinus communis (castor plant). It lethal in small quantities when disseminated as an aerosol. We determined impact using two types exposure chambers and different particle sizes on deposition ricin aerosols mice. Initially, inhalation (whole-body [WB] or nose-only [NO]) were compared same size aerosol (1 µm) to determine potential upon respiratory presented dose. then assessed role by with distinctly sized distributions. Selected organs...
TPS1143 Background: First identified as an estrogen-inducible gene in a breast cancer cell line, LIV-1 is multispan transmembrane protein of the solute-carrier family 39 with putative zinc transporter and metalloproteinase activity. As downstream target STAT3, it promotes epithelial-to-mesenchymal transition that important malignant progression to metastasis. expressed number cancers highest prevalence level expression breast, prostate, melanoma. Additionally, has been linked metastasis...
<div>Abstract<p>Nonclinical safety and pharmacokinetic data for monomethyl auristatin E (MMAE) 14 vedotin antibody–drug conjugates (ADC) were evaluated to determine patterns of toxicity, consistency results, species differences between rats monkeys. Most nonclinical toxicities antigen-independent, common across ADCs, included hematologic, lymphoid, reproductive toxicity related MMAE pharmacology. Hematologic was the dose-limiting (DLT) or predominant majority ADCs in both...
<p>Supplementary Figure 2. Reproductive toxicity with vedotin ADCs in cynomolgus monkeys.</p>
<p>Supplementary Figure 1. Hematopoietic and lymphoid tissue toxicity in cynomolgus monkeys administered a vedotin ADC or MMAE.</p>
Abstract Fucose is a sugar that widely incorporated in cellular glycoconjugates and has been implicated for roles tumor progression metastasis. An analog of fucose, 2-fluorofucose, shown to have activity vitro vivo as an inhibitor glycoconjugate fucosylation promising antitumor cancer growth models well potentiation the protective effect mouse B cell lymphoma idiotype vaccine against challenge by live A20 cells (Okeley N.M. et al, PNAS 110, 5404-5409, 2013). The effects 2-fluorofucose...
Supplementary Figures S1-S4 from Preclinical Characterization of SGN-70, a Humanized Antibody Directed against CD70