A5101728543- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Multiple Myeloma Research and Treatments
- Cancer therapeutics and mechanisms
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Radiopharmaceutical Chemistry and Applications
- Lymphoma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Connective tissue disorders research
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Cancer Immunotherapy and Biomarkers
- Protein Degradation and Inhibitors
- Skin and Cellular Biology Research
- T-cell and B-cell Immunology
- Biochemical and Structural Characterization
- Proteoglycans and glycosaminoglycans research
- Wnt/β-catenin signaling in development and cancer
- Peptidase Inhibition and Analysis
- Cancer Treatment and Pharmacology
- Synthesis and Biological Evaluation
- Autoimmune Bullous Skin Diseases
- Immunodeficiency and Autoimmune Disorders
Seagen (United States)
2010-2023
University of Washington
1990-2006
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
1994-2004
Fred Hutch Cancer Center
1991-2003
North Seattle College
2000
Chonnam National University
1999
Rush University Medical Center
1990
Presbyterian St. Luke's Medical Center
1990
United States Department of Veterans Affairs
1990
CD133/prominin-1 is a pentaspan transmembrane glycoprotein overexpressed in various solid tumours including colorectal and glioblastomas. CD133 was found here to be highly expressed >or=50% of pancreatic, gastric intrahepatic cholangiocarcinomas. Quantitative flow cytometric analysis showed that panel established hepatocellular, pancreatic cancer cell lines at levels higher than normal epithelial cells or bone marrow progenitor cells. A murine anti-human antibody (AC133) conjugated potent...
Laminin 5 regulates anchorage and motility of epithelial cells through integrins α6β4 α3β1, respectively. We used targeted disruption the LAMA3 gene, which encodes α3 subunit laminin other isoforms, to examine developmental functions that are regulated by adhesion basement membrane (BM). In homozygous null animals, profound abnormalities were detected resulted in neonatal lethality, consistent with removal all α3-laminin isoforms from BMs. Alterations three different cellular identified....
We have isolated cDNA clones encoding the entire 170-kDa chain of epiligrin (alpha 3Ep) and a genomic clone alpha 3Ep gene (LamA3). Analysis multiple revealed two distinct transcripts 3EpA 3EpB). Sequencing transcript indicated sequence structural homology to laminin 1 2 chains that extend from domain IIIa through carboxyl-terminal G domain. The 3EpB encodes larger amino-terminal contains additional epidermal growth factor repeats sequences corresponding IV laminin. Fluorescence in situ...
Type II collagen, like other fibrillar collagens, is synthesized as a procollagen containing amino (NH2)-and carboxyl (COOH)-terminal extension peptides. Based on cDNA cloning of human (Baldwin, C. T., Reginato, A. M., Smith, C., Jimenez, S. A., and Prockop, D. J. (1989) Biochem. 262, 521-528) rat (Kohno, K., Martin, G. R., Yamada, Y. (1984) Biol. Chem. 259, 13668-13673) type procollagen, it was concluded that much the NH2-terminal propeptide seen in pro-alpha 1(I) missing. Analysis genomic...
Metastatic renal cell carcinoma (RCC) is an aggressive disease refractory to most existing therapeutic modalities. Identifying new markers for progression and drug targets RCC will benefit this unmet medical need. We report a subset of clear papillary aberrantly expressing the lymphocyte activation marker CD70, member tumor necrosis factor superfamily. Importantly, CD70 expression was found be maintained at metastatic sites RCC. Anti-CD70 antibody-drug conjugates (ADC) consisting auristatin...
Abstract B-cell maturation antigen (BCMA) is expressed on normal and malignant plasma cells represents a potential target for therapeutic intervention. BCMA binds to two ligands that promote tumor cell survival, proliferation inducing ligand (APRIL) activating factor. To selectively malignancies, we developed antibodies with blocking activity could cytotoxicity of multiple myeloma (MM) lines as naked or antibody-drug conjugates. We show SG1, an inhibitory antibody, blocks APRIL–dependent...
CD70 is an ideal target for antibody-based therapies because of its aberrant high expression in renal carcinomas and non-Hodgkin lymphomas highly restricted normal tissues. The profiling has been limited the lack a CD70-specific reagent that works formalin-fixed paraffin-embedded (FFPE) We generated murine monoclonal antibodies (mAbs) specific validated their specificity by western blot analysis developed protocol immunohistochemistry on FFPE CD70+ tumour cell lines were used testing...
CD70 (CD27L) is a member of the tumor necrosis factor family aberrantly expressed on number hematologic malignancies and some carcinomas. expression malignant cells coupled with its highly restricted normal makes an attractive target for monoclonal antibody (mAb)-based therapies. We developed humanized anti-CD70 antibody, SGN-70, herein describe antitumor activities this mAb.CD70 primary tumors was evaluated by immunohistochemical staining Hodgkin lymphoma, non-Hodgkin multiple myeloma,...
In epidermal wounds, precursor laminin 5 (α3β3γ2) is deposited in the provisional basement membrane (PBM) before other BM components. Precursor contains G4/5 globular domains at carboxyl terminus of α3 chain. Here, function was evaluated deposition 5. Soluble 5, secreted by keratinocytes culture, cleaved an endogenous protease releasing G4/5. Thrombin, a serum protease, cleaves indistinguishably from protease. human but not bound and mouse null for chain (α3–/– MKs). The rescued adhesion...
We have developed a highly active and well-tolerated camptothecin (CPT) drug-linker designed for antibody-mediated drug delivery in which the lead molecule consists of 7-aminomethyl-10,11-methylenedioxy CPT (CPT1) derivative payload attached to novel hydrophilic protease-cleavable valine-lysine-glycine tripeptide linker. A defined polyethylene glycol stretcher was included improve properties drug-linker, facilitating high antibody-drug conjugate (ADC) loading, while reducing propensity...
Integrin beta-6, a component of the heterodimeric adhesion receptor alpha-v/beta-6, is overexpressed in numerous solid tumors. Its expression has been shown by multiple investigators to be negative prognostic indicator diverse cancers including colorectal, non-small cell lung, gastric, and cervical. We developed SGN-B6A as an antibody-drug conjugate (ADC) directed integrin beta-6 deliver clinically validated payload monomethyl auristatin E (MMAE) cancer cells. The antibody specific for does...