A5019334870- Analytical Chemistry and Chromatography
- Crystallization and Solubility Studies
- Chromatography in Natural Products
- Innovative Microfluidic and Catalytic Techniques Innovation
- Molecular Junctions and Nanostructures
- Gas Sensing Nanomaterials and Sensors
- Phase Equilibria and Thermodynamics
- Food Chemistry and Fat Analysis
- Chemical and Physical Properties in Aqueous Solutions
- Phytochemistry and biological activity of medicinal plants
TU Dortmund University
2021-2024
Centrifugal Partition Chromatography (CPC) utilizes a two-phase liquid–liquid system as mobile and stationary phases. During operation, the latter continuously drains out of rotor, despite it being in fact stationary, leading to decreasing separation efficiency over time, phenomenon still poorly understood today because neither simulations nor extensive experimental investigations have addressed this so-called bleeding. With model presented study, underlying hydrodynamics are discussed...
In downstream processes for peptides, crystallization is still used as the state-of-the-art separation step which knowledge about solubility of each single compound mandatory. Since determination experimental temperature-dependent data time-consuming and expensive, modeling based on physical properties such melting highly desired. Unfortunately, direct biomolecules using conventional differential scanning calorimetry not possible due to decomposition peptides before their melting. this work,...
Centrifugal Partition Chromatography is a type of Liquid-Liquid that offers several advantages compared to Liquid Chromatography. Two immiscible liquids are utilized, and one phase has be immobilized implement chromatographic separation. This performed with the help centrifugal force. As this immobilization not ideal, stationary continuously leaks out apparatus (so-called bleeding). We measured phase’s loss precisely implemented controller compensate for it during operation. innovative mode...
In this work the solubility of 15 amino acids and 18 peptides in aqueous 2-propanol solutions was successfully modelled using PC-SAFT that used recently determined experimental melting properties as input data.