A5114608923- bioluminescence and chemiluminescence research
- CRISPR and Genetic Engineering
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
Zhejiang University
2023-2024
Summary The fungal bioluminescence pathway (FBP) was identified from glowing fungi, which releases self‐sustained visible green luminescence. However, weak limits the potential application of system. Here, we screened and characterized a C3′H1 (4‐coumaroyl shikimate/quinate 3′‐hydroxylase) gene Brassica napus , efficiently converts p ‐coumaroyl shikimate to caffeic acid hispidin. Simultaneous expression BnC3′H1 NPGA (null‐pigment mutant in A. nidulans ) produces more hispidin as natural...
Abstract Autoluminescent plants have been genetically modified to express the fungal bioluminescence pathway (FBP). However, a bottleneck in precursor production has limited brightness of these luminescent plants. Here, we demonstrate effectiveness utilizing computational model guide multiplex five-gene-silencing strategy by an artificial microRNA array enhance caffeic acid (CA) and hispidin levels By combining loss-of-function-directed metabolic flux with tyrosine-derived CA pathway,...