The high level of escapes from Atlantic salmon farms, up to two million fishes per year in the North Atlantic, has raised concern about potential impact on wild populations. We report a two-generation experiment examining estimated lifetime successes, relative natives, farm, F1 and F2 hybrids BC1 backcrosses farm salmon. Offspring ‘hybrids’ (i.e. all F1, groups) showed reduced survival compared with but grew faster as juveniles displaced parr, which group were significantly smaller. Where...

An efficient total DNA isolation protocol, suitable for routine population genetic screening purposes is described. This phenol based extraction can utilize fresh, frozen or ethanol preserved tissues.

The main molecular techniques which can be used to generate genetic markers, and the applications of these markers studies fish populations are outlined. Published ongoing studies, in authors' laboratories, on brown trout Atlantic salmon compare resolution applicability allozyme, mitochondrial DNA minisatellite (variable number tandem repeats) for population structuring, variation within populations, impact accidental deliberate introduction non‐native salmonids make‐up natural populations.

The lifetime success and performance characteristics of communally reared offspring wild native Burrishoole (native), ranched (ranched) non‐native (non‐native) Atlantic salmon Salmo salar from the adjacent Owenmore River were compared. Non‐native 0+ year parr showed a substantial downstream migration, which was not shown by parr. This appears to have been an active migration rather than competitive displacement may reflect adaptation environmental or physiographic conditions within catchment...

The phylogeographical structure of brown trout Salmo trutta in Britain and Ireland was studied using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis four mitochondrial DNA segments (16S/ND1, ND5/6, COXIII/ND5 ND5/12S). Analysis 3636 individuals from 83 sites-morphotypes revealed a total 25 haplotypes. These haplotypes were nested seven two-step clades. Although there clear geographical patterning to the occurrence derived clades, admixture among...

A purified mitochondrial DNA (mtDNA) probe was used to examine restriction fragment length polymorphisms produced by six enzymes ( Xba I, Eco RV, Ava II, Hinf Hae III, Mbo I) in 915 brown trout from western Europe. total of 20 composite haplotypes were found with one seven individual populations. Icelandic samples north, south, east, and west coast drainages showed only a single common haplotype contrast the high level polymorphism Irish Scottish The phylogeny mtDNA pattern distribution...

Despite recent advances in the understanding of interplay between a dynamic physical environment and phylogeography Europe, origins contemporary Irish biota remain uncertain. Current thinking is that Ireland was colonized post-glacially from southern European refugia, following end last glacial maximum (LGM), some 20 000 years BP. The Leisler's bat (Nyctalus leisleri), one few native mammal species, widely distributed throughout Europe but, with exception Ireland, generally rare considered...

Abstract Declines in the number of anadromous brown trout Karup River Denmark, due to environmental degradation, led stocking large numbers hatchery during 1980s. This practice was gradually replaced by with offspring electrofished local The genetic contribution fish current population river estimated restriction fragment length polymorphism analysis mitochondrial DNA, using seven endonucleases. Fish from strain as well five locations system, and a further unstocked were screened. Eight...

RFLP analysis of mitochondrial DNA (mtDNA) was used to study variation within and between ferox, sonaghen, gillaroo, the local names three types brown trout, Salmo trutta L., which live sympatrically in Lough Melvin, northwest Ireland. They spawn separate rivers or sections thus maintain reproductive integrity by their innate homing behaviour. In this study, 12 restriction enzymes were survey 4.9% genome 37 trout. Five informative (AvaII, HaeIII, HinfI, MboI, XbaI) these examine an...

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 421:173-182 (2011) - DOI: https://doi.org/10.3354/meps08895 Genetic parentage in squat lobsters Munida rugosa and M. sarsi (Crustacea, Anomura, Galatheidae) Deborah A. Bailie, Rosaleen Hynes, Paulo Prodöhl* School of Biological Sciences, Queen’s University Belfast, Medical Biology Centre, 97 Lisburn Road,...

A flexible panel consisting of 38 informative microsatellite markers for Salmo trutta is described. These were selected from a pool over 150 candidate loci that can be readily amplified in four multiplex PCR groups but other permutations are also possible. The basic properties each assessed six population samples both the Burrishoole catchment, west Ireland, and Lough Neagh, Northern Ireland. method to assess relative utility individual detection genetic structuring Given its flexibility,...

Mitochondrial DNA was examined in natural and hatchery‐reared stocks of brown trout, using different methods restriction analysis. The included the development a trout mt hybridization probe through cloning part mitochondrial genome. In addition, fragments were analysed by ethidium bromide staining end‐labelling. relative merits each these assessing levels genetic relatedness between evaluated. study revealed diagnostic mtDNA pattern which could be used as marker for discrimination two groups trout.

Contact zones between divergent forms of the same species are often characterised by high levels phenotypic diversity over small geographic distances. What processes involved in generating such diversity? One possibility is that introgression and recombination contact results greater genetic polymorphism. Alternatively, strong reproductive isolation may maintain distinct phenotypes, preventing homogenisation gene flow. freshwater-resident anadromous stickleback (Gasterosteus aculeatus L.)...

Over the past decade, common rock shrimp, Rhynchocinetes typusH. Milne Edwards, 1837, has been focus of extensive investigations on mating behaviour. The species is now perceived as a model system for study reproductive strategies and sexual conflict in crustaceans displaying external fertilization. Using molecular markers, current assesses whether social behaviour shrimp translates into true genetic parentage. In large mesocosm tank with |$\gt 200$| individuals both sexes, analysis 15...

Abstract Anthropogenic acidification in SW‐Scotland, from the early 19th Century onwards, led to extinction of several loch (lake) brown trout ( Salmo trutta ) populations and substantial reductions numbers many others. Higher altitude with no stocking influence, which are isolated above natural artificial barriers subjected greatest effect acidification, exhibited least intrapopulation genetic diversity (34% allelic richness accessible anadromous S. ). These, however, were characterised by...

Abstract Introduced non-native species can threaten native through interspecific hybridisation and genetic introgression. We assessed the prevalence of introgression between introduced European brown hare, Lepus europaeus , endemic Irish L. timidus hibernicus . Roadkill hares ( n = 56) were sequenced for a 379bp section mitochondrial DNA D-loop 474bp segment nuclear transferrin Tf ) gene. A species-specific indel in gene was present L.t. absent Excluding three from which molecular data could...

Plant growth promotion by Pseudomonas sp. 31-12 incorporated into a lettuce seed pelleting matrix was studied. We examined (1) the effect of five rhizosphere derived bacterial strains on green oak ( Lactuca sativa L.) germination, root and shoot growth, as strain selection step for coating pelletizing studies, (2) population stability stored three months at 4 °C, (3) in laboratory greenhouse coated pelletized seed. A spontaneous streptomycin mutant (str) used to determine size roots 15-...