A5052089699- Wound Healing and Treatments
- Dermatologic Treatments and Research
- Porphyrin Metabolism and Disorders
- Hemoglobin structure and function
- Organ Transplantation Techniques and Outcomes
- Plant Molecular Biology Research
- Cardiac Ischemia and Reperfusion
- Congenital heart defects research
- Plant tissue culture and regeneration
- Erythrocyte Function and Pathophysiology
- Pulmonary Hypertension Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Plant Genetic and Mutation Studies
- Electrospun Nanofibers in Biomedical Applications
- Anesthesia and Neurotoxicity Research
- Silk-based biomaterials and applications
- Hair Growth and Disorders
- Cardiac Fibrosis and Remodeling
University of Indonesia
2019-2024
This protocol introduces a streamlined and efficient method for isolating human fibroblast from skin primary cell culture with specific focus on its application to keloid, hypertrophic scar, normal biopsies. Additionally, the absence of suitable animal models keloid scar has led preclinical research rely in vitro studies using cultures. approach addresses challenges existing protocols terms time, cost, equipment, technical expertise required. The involves derivation, culture,...
The healing of skin wounds involves various factors including keratinocytes, fibroblasts, extracellular matrix, nervous and vascular components the surrounding tissue. During wound healing, keratinocytes migrate from basal area to cover lesion area. important role in regeneration triggers interests isolate expand cells vitro. This study was attempted compare briefly enzymatic non-enzymatic isolation adult skin. Non-enzymatic based keratinocyte through explant culture allowed minimal sample...
Abstract Melastoma malabathricum L. is a plant potentially useful in phytoremediation, and its successful propagation would facilitate further research this application. The present study examined vitro culture of leaf explants using MS medium supplemented with thidiazuron (TDZ; 0, 0.1, 1 2 mg/L) 1-naphthaleneacetic acid (NAA; 0.1 alone combination. Leaf responded to all treatments by forming callus, except on combination mg mg/L TDZ NAA. callus formed tended be friable-compact compact...
Heme is a compound consisting of an iron (Fe) atom bound to pyrrole ring forming protoporphyrin IX (PPIX). Protoporphyrin combines with protein-forming hemoprotein that plays essential role in oxygen-binding and transport as well the process energy production mitochondria. Some cancer cells have more heme biosynthesis than normal cells, which thought be linked cell growth. Inhibition some leads decreased proliferation. This review article discusses synthesis heme, metabolism, needed for...
The aim of this study was to analyze the effect heme synthesis inhibition on cytoglobin expression and its correlation with keloid fibroblast viability proliferation. conducted primary culture fibroblasts. Heme in fibroblasts inhibited using succinyl acetone. We measured ALAD enzyme activity a colorimetric method; mRNA qRT-PCR; protein ELISA immunocytochemistry; MTT test; proliferation BrdU test. results showed that level lower treated SA (1, 2.5, 5 mM) than control. CYGB expressions were...
Objective: The platelet-rich fibrin matrix (PRFM) is condensed plasma (PRP) and should possess a comparable biomechanical property to the transplanted sites, for them be physiologically functional. aim of this study was investigate effect human bone marrow mesenchymal stem cells (hBM-MSC) or umbilical cord (hUC-MSC) on properties PRFM.
 Methods: PRFM prepared by gelation PRP using 25 mmol CaCl2. resulting coin-shaped pellets, 5 cm in diameter 300 μm thick, were directly seeded with...