A5026484851- Angiogenesis and VEGF in Cancer
- Nerve injury and regeneration
- Wound Healing and Treatments
- Estrogen and related hormone effects
- 3D Printing in Biomedical Research
- Per- and polyfluoroalkyl substances research
- Sarcoma Diagnosis and Treatment
- Nitric Oxide and Endothelin Effects
- Pluripotent Stem Cells Research
- Cardiac Fibrosis and Remodeling
- Virus-based gene therapy research
- Birth, Development, and Health
- Connexins and lens biology
- Nuclear Receptors and Signaling
- Tendon Structure and Treatment
- Reproductive Biology and Fertility
- Yersinia bacterium, plague, ectoparasites research
- Hereditary Neurological Disorders
- Histone Deacetylase Inhibitors Research
- Renin-Angiotensin System Studies
- Chemotherapy-induced cardiotoxicity and mitigation
- Vestibular and auditory disorders
- Spinal Cord Injury Research
- Musculoskeletal synovial abnormalities and treatments
- Hearing Loss and Rehabilitation
University of Padua
2021-2024
Istituto Ortopedico Rizzoli
2022-2024
Veneto Institute of Molecular Medicine
2023
Fondazione IRET ONLUS
2020-2022
University of Bologna
2017-2020
Chiesi (Italy)
2020
Hadassah Medical Center
2015-2017
University of Ferrara
2013-2015
Arcispedale Sant'Anna
2014
Perfluoroalkyl substances (PFASs) are synthetic chemicals widely used in industrial and consumer products. The environmental spreading of PFASs raises concerns for their impact on human health. In particular, the bioaccumulation humans due to exposure has been reported also total brain samples PFAS associated with neurodevelopmental disorders. this study we aimed investigate specific different areas. Our data major accumulation brainstem region, which is richly populated by dopaminergic...
Estrogens play a protective role in coronary artery disease. The mechanisms of action are still poorly understood, although for estrogens stimulation angiogenesis has been suggested. In several cell types, modulate the Notch pathway, which is involved controlling downstream vascular endothelial growth factor A (VEGF-A). goal our study was to establish whether activity cells and possible consequences on angiogenesis. Human umbilical vein (HUVECs) were treated with 17β-estradiol (E2) effects...
Unlike age-matched men, premenopausal women benefit from cardiovascular protection. Estrogens protect against apoptosis of endothelial cells (ECs), one the hallmarks dysfunction leading to disorders, but underlying molecular mechanisms remain poorly understood. The inflammatory cytokine TNFα causes EC while dysregulating Notch pathway, a major contributor survival. We have previously reported that 17β-estradiol (E2) treatment activates signaling in ECs. Here, we sought assess whether...
Nerve growth factor (NGF) is recognized as a pleiotropic molecule, exerting variety of biological effects on different cell types and pathophysiological conditions, its role in tissue wound healing has been recently highlighted. However, the preferential cellular target NGF still elusive complex molecular cross talk that accompanies healing. Thus, to explore possible targets skin healing, we investigated vitro responsiveness keratinocytes (cell line HEKa), fibroblasts BJ), endothelial cells...
In myelinating Schwann cells, connection between myelin layers is mediated by gap junction channels (GJCs) formed docked connexin 32 (Cx32) hemichannels (HCs). Mutations in Cx32 cause the X-linked Charcot-Marie-Tooth disease (CMT1X), a degenerative neuropathy without cure. A molecular link dysfunction and CMT1X pathogenesis still missing. Here, we describe high-resolution cryo-electron microscopy (cryo-EM) structures of GJC HC, along with two CMT1X-linked mutants, W3S R22G. While wild-type...
Experimental models for chronic skin lesions are excision and pressure ulcer, defined as "open" "closed" lesions, respectively, only the latter characterized by tissue hypoxia. Moreover, systemic diseases, such diabetes mellitus, affect wound repair. Thus, testing new therapies should be carefully selected according to expected targets. In this study, we present an extensive comparative histological, immunohistochemical, molecular characterization of these two in diabetic (db/db)...
The recent increase in human lifespan, coupled with unhealthy diets and lifestyles have led to an unprecedented cardiovascular diseases. Even the presence of a wide range therapeutic options variable efficacy, mortality due heart failure is still high there need identify new targets. Genetic vitro studies implicated Notch signalling development maintenance system through direct effect on biological functions vascular cells (endothelial smooth muscle cells) cardiomyocytes. also involved...
The lack of early diagnosis, progression markers and effective pharmacological treatment has dramatic unfavourable effects on clinical outcomes in patients with peripheral artery disease (PAD). Addressing these issues will require dissecting the molecular mechanisms underlying this disease. We sought to characterize Notch signaling atherosclerosis relevant lesions from femoral arteries symptomatic PAD patients.Plaque material common femoral, superficial or popliteal 20 was removed by...
Cell-based in vitro biological models traditionally use monolayer cell cultures grown over plastic surfaces bathing static media. Higher fidelity to a natural tissue is expected result from growing the cells three-dimensional (3D) matrix. However, due decreased rate of diffusion inherent increased distances within tridimensional space, proper fluidic conditions are needed this setting better approximate physiological environment. To aim, we here propose prototypal dynamic culture platform...
Spinal cord injury (SCI) is an incurable condition, in which a cascade of cellular and molecular events triggered by inflammation excitotoxicity impairs endogenous regeneration, namely remyelination axonal outgrowth. We designed treatment solution based on implantable biomaterial (electrospun poly (l-lactic acid) [PLLA]) loaded with ibuprofen triiodothyronine (T3) to counteract inflammation, thus improving regeneration. In vivo efficacy was tested implanting the drug-loaded PLLA rat model T8...
Tissue-engineered implants for bone regeneration require consideration regarding their mineralization and vascularization capacity. Different geometries, such as biomimetic designs lattices, can influence the mechanical properties capacity of bone-mimicking implants. Negative Embodied Sacrificial Template 3D (NEST3D) printing is a versatile technique across wide range materials that enables production scaffolds. In this study, different scaffold motifs (logpile, Voronoi, trabecular bone)...
It is unknown whether components present in heart failure (HF) patients’ serum provide an angiogenic stimulus. We sought to determine from HF patients affects angiogenesis and its major modulator, the Notch pathway, human umbilical vein endothelial cells (HUVECs). In treated with healthy subjects or at different stage we determined: (1) Sprouting angiogenesis, by measuring network (closed tubes) collagen gel. (2) Protein levels of receptors 1, 2, 4, ligands Jagged1, Delta‐like4. found a...
Although myocardial angiogenesis is thought to play an important role in heart failure (HF), the involvement of circulating proinflammatory and proangiogenic cytokines pathogenesis and/or prognosis HF has not been deeply investigated. By using a highly standardized proliferation assay with human endothelial cells, we first demonstrated that sera from older (mean age<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Nerve growth factor (NGF) is the protein responsible for development and maintenance of sensory skin innervation. Given role appropriate innervation in healing, NGF has been indicated as a possible prohealing treatment pathologic conditions characterized by nerve-ending loss, such chronic ulcers diabetes; however, its use therapeutic agent limited hyperalgesic effect. We tested effect topical application nonalgogenic derivative hNGFP61S/R100E two models ulcer induced <i>dbdb</i> diabetic...
While the role of thyroid hormones (THs) during fetal and postnatal life is well-established, their at preimplantation blastocyst development remains unclear. In this study, we used an embryonic stem cell line isolated from rat (RESC) to study effects THs retinoic acid (RA) on early pre-implantation stage. The results showed that play important in differentiation/maturation processes cells obtained embryoid bodies (EB), with hormone nuclear receptors (TR) (TRα TRβ), metabolic enzymes...
Perfluoro–alkyl substances (PFAS) are chemical pollutants with prevalent stability and environmental persistence. Exposure to PFAS, particularly perfluoro-octanoic acid (PFOA), has been associated increased diabetes-related cardiovascular mortality in subjects residing areas of high contamination, however the exact pathogenic mechanism remains elusive. Here we used HepG2 cells, an vitro model human hepatocyte, investigate possible role PFOA exposure alteration hepatic glucose metabolism....
Abstract Background Myxofibrosarcoma is a rare malignant soft tissue sarcoma characterised by multiple local recurrence and can become of higher grade with each recurrence. Consequently, myxofibrosarcoma represents burden for patients, challenge clinicians, an interesting disease to study tumour progression. Currently, few preclinical models are available. Methods In this paper, we present spontaneously immortalised patient-derived cell line (MF-R 3). We performed phenotypic characterization...