A5103041659- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- X-ray Spectroscopy and Fluorescence Analysis
- Electron and X-Ray Spectroscopy Techniques
- Carcinogens and Genotoxicity Assessment
- Radiation Effects and Dosimetry
- Ion-surface interactions and analysis
- Radiation Therapy and Dosimetry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Advanced X-ray Imaging Techniques
- Radiation Shielding Materials Analysis
- CRISPR and Genetic Engineering
- Boron Compounds in Chemistry
- Drug Transport and Resistance Mechanisms
- Biopolymer Synthesis and Applications
- Molecular Biology Techniques and Applications
- Metal complexes synthesis and properties
- Mass Spectrometry Techniques and Applications
- bioluminescence and chemiluminescence research
- Cancer therapeutics and mechanisms
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Laser-Ablation Synthesis of Nanoparticles
National Institutes for Quantum Science and Technology
2016-2025
Japan Atomic Energy Agency
2001-2015
Kansai Photon Science Institute
2012-2015
Japan Society for the Promotion of Science
2003-2004
Japan Synchrotron Radiation Research Institute
2001-2004
SPring-8
2001-2004
Japan Science and Technology Agency
2003-2004
Advanced Science Research Center
2004
Kyoto University
1997-1999
SignificanceDNA damage causes loss of or alterations in genetic information, resulting cell death mutations. Ionizing radiations produce local, multiple DNA sites called clustered damage. In this study, a complete protocol was established to analyze the complexity damage, wherein damage-containing genomic fragments were selectively concentrated via pulldown, and visualized by atomic force microscopy. It found that X-rays Fe ion beams caused also produced with high complexity. beam-induced...
The abasic site (apurinic/apyrimidinic site) is the most common lesion in DNA and suggested to be an important intermediate mutagenesis carcinogenesis. We have recently reported a novel assay for detection quantitation of sites [Kubo, K., Ide, H., Wallace, S. S., & Kow, Y. W. (1992) Biochemistry 31, 3703-3708]. In this assay, aldehyde group first modified by probe bearing biotin residue, called Aldehyde Reactive Probe (ARP) then tagged quantified ELISA-like assay. However, previous study,...
Sequence-dependent mechanical properties of DNA could play essential roles in nuclear processes by affecting histone-DNA interactions. Previously, we found that the entry site first nucleosomes from transcription start (+ 1 nucleosome) budding yeast enriches AA/TT steps, but not exit site, and biased presence was associated with levels genes. Because is a rigid dinucleotide step, considered causes unwrapping. However, our previous MNase-seq experiments reconstituted left some doubt regarding...
The near-edge X-ray absorption fine structure (NEXAFS) of evaporated thin films DNA nucleobases on an Au-coated Si surface at room temperature has been investigated in the regions around nitrogen and oxygen K-edges using highly monochromatic synchrotron soft X-rays. Each nucleobase shows a specific spectrum originating from resonant excitation 1s electrons or to antibonding states, some which strongly depend polarization angle incident From analysis dependences π* resonance intensity, it is...
Complex DNA damage, defined as at least two vicinal lesions within 10-20 base pairs (bp), induced after exposure to ionizing radiation, is recognized fatal damage human tissue. Due the difficulty of directly measuring aggregation nano-meter scale, many cluster analyses inelastic interactions based on Monte Carlo simulation for radiation track structure in liquid water have been conducted evaluate damage. Meanwhile, experimental technique detect complex has evolved recent decades, so both...
Clustered DNA damage is considered an important factor in determining the biological consequences of ionizing radiation. In this study, we successfully estimated localization abasic sites (APs) exposed to radiation using fluorescence resonance energy transfer (FRET) without any involvement repair enzyme functions. A linearized plasmid (pUC19 digested by Sma I) was irradiated with: (60)Co γ rays; (4)He(2+) (2.0 MeV/u) particles; and (12)C(5+) (0.37 particles solid state. donor or acceptor...
. Time-dependent yields of chemical products resulting from water radiolysis play a great role in evaluating DNA damage response after exposure to ionizing radiation. Particle and Heavy Ion Transport code System (PHITS) is general-purpose Monte Carlo simulation for radiation transport, which simulates atomic interactions originating discrete energy levels ionizations electronic excitations as well molecular physical stages. However, no simulating exists the PHITS package.
Potentials of glycosylated poly(amino acids) as carriers drugs and/or polynucleotides to the liver were studied in mice detail. Poly-L-glutamic acid (PLGA) and poly-L-lysine (PLL) selected carrier backbones modified with 2-imino-2-methoxyethyl 1-thiogalactoside or mannoside obtain galactosylated mannosylated derivatives. After intravenous injection at a dose 1 mg/kg, Gal-PLGA Man-PLGA selectively taken up by parenchymal cells (PC) nonparenchymal (NPC), respectively. Moreover, uptake...
The initial process of radiation damage in DNA was investigated by measuring the X-ray absorption near edge structures (XANES) within energy region around oxygen K-shell for DNA, cytosine and 2-deoxy-d-ribose. Irradiation XANES experiments were performed with BL23SU soft beamline, using synchrotron from 8 GeV electron storage ring at SPring-8. Samples mounted on gold-coated plates a vacuum chamber. spectra obtained photoelectron current samples. 2-Deoxy-d-ribose exposed to X rays peak...
Purpose: To understand the characteristics of DNA damage induced by Auger effect in ultrasoft X-irradiation. In situ electron paramagnetic resonance (EPR) spectroscopy as well biochemical analysis has been applied to examine induction both viewpoints intermediate species and final products.Materials methods: Unpaired a calf thymus film irradiated with monochromatic X-rays (270–580 eV) was observed using an X-band EPR spectrometer installed synchrotron beamline. determine yield single strand...
Positive ion desorption from thin films of DNA components, 2-deoxy-d-ribose, thymine, thymidine (dThd), and 5'-monophosphate (dTMP) was investigated in the oxygen K- shell edge excitation region using synchrotron ultrasoft X rays (538 eV). A large number molecular fragments, H(+), CH(x)(+), C(2)H(x)(+), CO(+), CH(x)O(+), C(3)H(x)(+), C(2)H(x)O(+) C(3)H(x)O(+) (x = 1, 2 3), were observed as desorbed ions 2-deoxy-d-ribose. Some these are related to simultaneous bond scission at particular C-C...