A5068263408- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Plant Genetic and Mutation Studies
- Plant biochemistry and biosynthesis
- Fungal and yeast genetics research
- Bacterial biofilms and quorum sensing
- Studies on Chitinases and Chitosanases
- Biofuel production and bioconversion
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Chemical synthesis and alkaloids
- Enzyme Production and Characterization
- Nematode management and characterization studies
- Plant pathogens and resistance mechanisms
- Mollusks and Parasites Studies
- GABA and Rice Research
- Enzyme Catalysis and Immobilization
- Fungal Biology and Applications
Institute of Agrobiological Sciences
2007-2024
National Agriculture and Food Research Organization
2017-2022
University of Tsukuba
2006
Chitin is a major component of fungal cell walls and serves as molecular pattern for the recognition potential pathogens in innate immune systems both plants animals. In plants, chitin oligosaccharides have been known to induce various defense responses wide range plant cells including monocots dicots. To clarify machinery involved perception transduction oligosaccharide elicitor, high-affinity binding protein this elicitor was isolated from plasma membrane suspension-cultured rice cells....
Pseudomonas syringae strains deliver diverse type III effector proteins into host cells, where they can act as virulence factors. Although the functions of majority effectors are unknown, several have been shown to interfere with plant basal defense mechanisms. Type also could contribute bacterial by enhancing nutrient uptake and pathogen adaptation environment plant. We demonstrate that HopAM1 (formerly known AvrPpiB) enhances a weak in plants grown under drought stress. This is first...
Xanthomonas oryzae pv. is the causal agent of bacterial blight rice. The XopR protein, secreted into plant cells through type III secretion apparatus, widely conserved in xanthomonads and predicted to play important roles pathogenicity. Here, we examined function by constructing transgenic Arabidopsis thaliana plants expressing it under control dexamethasone (DEX)-inducible promoter. In treated with DEX, slightly delayed growth variegation on leaves were observed. Induction four...
Expression of OsWRKY71, a rice WRKY gene, was induced by biotic elicitors and pathogen infection. It also found that OsWRKY71 has features characteristic transcriptional repressor. Microarray analysis revealed several elicitor-induced defense-related genes were upregulated in cells overexpressing OsWRKY71. These results indicate the activation probably indirect.
Summary Protein phosphatases (PPs) counteract kinases in reversible phosphorylation events during numerous signal transduction pathways eukaryotes. PP2Cs, one of the four major classes serine/threonine‐specific PP family, are greatly expanded plants. Thus, PP2Cs thought to play a specific role pathways. Some rice classified subgroup K responsive infection by compatible Xanthomonas oryzae pv. , causal agent bacterial blight. In Arabidopsis thaliana orthologous PP2C genes ( AtPP2C62 and...
Seedling rot, caused by the bacterial pathogen Burkholderia glumae, is a major disease of rice. It originates from pathogen-contaminated seeds and thus mainly controlled pesticide treatments seeds. We previously demonstrated that seed-borne bacteria rice may be useful sustainable alternative to pesticides manage seedling but they are limited in terms variety. Here, we report another bacterium, Pantoea dispersa BB1, protects B. glumae. screened 72 isolates three genetically different...
Xanthomonas is one of the most widespread phytobacteria, causing diseases on a variety agricultural plants. To develop novel control techniques, knowledge bacterial behavior inside plant cells essential. campestris pv. campestris, vascular pathogen, causal agent black rot leaves Brassicaceae, including Arabidopsis thaliana. Among X. stocks in MAFF collection, we selected XccMAFF106712 as model compatible pathogen for A. thaliana reference ecotype Columbia (Col-0). Using modified green...
Abstract Burkholderia glumae is a causal agent of bacterial grain and seedling rot in rice, threat to stable global food supply. The virulence B. was suppressed when it inoculated on budding seed rather than non-budding seed. To clarify the phenomena, pathogen titer inside rice plant measured by serial dilution plating lysates from seedlings. Surprisingly, morphologically different types colonies were observed plates. These ‘contaminated’ seed-born bacteria (RSB) identified sequencing 16S...
Abstract Many factors produced by the pathogen Magnaporthe oryzae enhance its ability to infect rice. We found a novel infection‐promoting activity in supernatant of conidia suspension (SCS) M . The addition SCS promoted invasion excised rice leaf sheaths infectious hyphae. was heat‐stable and SCSs from five virulent three avirulent isolates on cv. Nipponbare ( Pia ). effect exclusively detected compatible interactions. infection plants non‐rice blast fungi not enhanced SCS. These results...
We previously detected infection-promoting activity in the supernatant of conidial suspension (SCS) rice blast fungus. In present study, a molecule carrying was purified and identified as 2'-deoxyuridine (dU). The dU strictly dependent on its chemical structure displayed characteristics consistent with those SCS. Notably, exclusively during interactions between virulent isolates fungus, number susceptible lesions leaf blades increased by dU, nonhost resistance plants not affected treatment...
To isolate genes responsive to N-acetylchitooligosaccharide elicitor in suspension-cultured rice cells, we screened ESTs by DNA microarray analysis, and were able identify novel elicitor-responsive genes. Sequencing analysis of three revealed that the up-regulated include a transcription factor, Myb, small G-protein, Rac, calmodulin. The results indicated genome-wide change gene expression response elicitor.
Rice seed-borne diseases caused by the bacterial pathogens Burkholderia glumae and B . plantarii pose a major threat to rice production worldwide. To manage these in sustainable manner, biocontrol strategy is crucial. In this study, we showed that gladioli NB6 (NB6), nonpathogenic bacterium, strongly protects from infection above-mentioned pathogens. was isolated indica cultivar Nona Bokra seedlings, which possesses genetic resistance We discovered cell suspensions of its culture filtrate...