A5089799055- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Plant Disease Management Techniques
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Plant Stress Responses and Tolerance
- Bacterial biofilms and quorum sensing
- Plant Reproductive Biology
- Plant Virus Research Studies
- Radiation Dose and Imaging
- Additive Manufacturing and 3D Printing Technologies
- Dental Radiography and Imaging
- Biochemical Analysis and Sensing Techniques
- Plant Gene Expression Analysis
- Mycorrhizal Fungi and Plant Interactions
- Plant and Fungal Interactions Research
- Glycosylation and Glycoproteins Research
- Dental Implant Techniques and Outcomes
- 3D Printing in Biomedical Research
- Studies on Chitinases and Chitosanases
- Genomics and Phylogenetic Studies
Institute of Agrobiological Sciences
2011-2025
National Agriculture and Food Research Organization
2014-2025
Kanagawa Dental University
2022-2024
RIKEN Center for Sustainable Resource Science
2017-2023
Okayama University
2016-2022
Kihara Institute for Biological Research
2020
Yokohama City University
2020
University of Tsukuba
2011
Plants are constantly exposed to threats from pathogenic microbes and thus developed an innate immune system protect themselves. On the other hand, many plants also have ability establish endosymbiosis with beneficial such as arbuscular mycorrhizal (AM) fungi or rhizobial bacteria, which improves growth of host plants. How evolved these systems managing opposite plant–microbe interactions is unclear. We show here that knockout (KO) mutants OsCERK1, a rice receptor kinase essential for chitin...
Plants evoke innate immunity against microbial challenges upon recognition of pathogen-associated molecular patterns (PAMPs), such as fungal cell wall chitin. Nevertheless, pathogens may circumvent the host PAMP-triggered immunity. We previously reported that ascomycete Magnaporthe oryzae, a famine-causing rice pathogen, masks surfaces with α-1,3-glucan during invasion. Here, we show surface is indispensable for successful infection fungus by interfering plant's defense mechanisms. The...
Chitin is a component of fungal cell walls, and its fragments act as elicitors in many plants. The plasma membrane glycoprotein CEBiP, which possesses LysM domains, receptor for the chitin elicitor (CE) rice. Here, we report that perception CE by CEBiP contributes to disease resistance against rice blast fungus, Magnaporthe oryzae, enhanced responses engineering increase tolerance. Knockdown expression allowed increased spread infection hyphae. To enhance defense CE, constructed chimeric...
OsCERK1 is a rice receptor-like kinase that mediates the signal of fungal cell wall component, chitin, by coordinating with lysin motif (LysM)-containing protein CEBiP. To further elucidate function in defense response, we disrupted using an Agrobacterium-mediated gene targeting system based on homologous recombination. In OsCERK1-disrupted lines, generation hydrogen peroxide and alteration expression response to chitin oligomer were completely abolished. The lines also showed lowered...
Summary Rhizoctonia solani is a soil‐borne fungus causing sheath blight. In consistent with its necrotrophic life style, no rice cultivars fully resistant to R. are known, and agrochemical plant defense activators used for blast, which upregulate phytohormonal salicylic acid (SA)‐dependent pathway, ineffective towards this pathogen. As result of the unavailability genetics, infection process remains unclear. We model monocotyledonous plants Brachypodium distachyon rice, evaluated effects...
Summary Plant cell surface receptor‐like kinases ( RLK s) mediate the signals from microbe‐associated molecular patterns MAMP that induce immune responses. Lipopolysaccharide LPS ), major constituent of outer membrane gram‐negative bacteria, is a common perceived by animals and plants; however, plant receptors/co‐receptors are unknown except for LORE , bulb‐type lectin S‐domain (B‐lectin SD 1‐ ) in Arabidopsis. Os CERK 1 multifunctional rice contains lysin motifs (LysMs) essential perception...
Tomato brown rugose fruit virus (ToBRFV) is an emerging of the genus Tobamovirus. ToBRFV overcomes tobamovirus resistance gene Tm-22 and rapidly spreading worldwide. Genetic resources for are urgently needed. Here, we show that clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9)-mediated targeted mutagenesis four tomato (Solanum lycopersicum) homologs TOBAMOVIRUS MULTIPLICATION1 (TOM1), Arabidopsis (Arabidopsis thaliana) essential...
Brachypodium distachyon is a promising model plants for grasses. Infections of by various pathogens that severely impair crop production have been reported, and the species accordingly provides an alternative platform investigating molecular mechanisms pathogen virulence plant disease resistance. To date, we broad picture immunity only in Arabidopsis rice; therefore, may constitute counterpart displays commonality uniqueness defence systems among species. Phytohormones play key roles biotic...
Rhizoctonia solani is a basidiomycete phytopathogenic fungus that causes rapid necrosis in wide range of crop species, leading to substantial agricultural losses worldwide. The species complex divided into 13 anastomosis groups (AGs) based on hyphal fusion compatibility and further subdivided by culture morphology. While R. classifications were shown be independent host specificity, it remains unclear whether different isolates share similar virulence mechanisms. Here, we investigated the...
Rhizoctonia solani is a soil-borne necrotrophic fungus that causes sheath blight in grasses. The basal resistance of compatible interactions between R. and rice known to be modulated by some WRKY transcription factors (TFs). However, genes defense responses involved incompatible interaction with remain unexplored, because no such are any host plants. Recently, we demonstrated Bd3-1, an accession the model grass Brachypodium distachyon, resistant and, upon inoculation fungus, undergoes rapid...
Bacterial canker of tomato (Solanum lycopersicon) caused by the Gram-positive bacterium Clavibacter michiganensis subsp. (Cmm) is an economically important disease. To understand host defense response to Cmm infection, transcriptome sequences in cotyledons were analyzed RNA-seq. Overall, 1788 and 540 genes upregulated downregulated upon respectively. Gene Ontology enrichment analysis revealed that involved response, phosphorylation, hormone signaling over-represented infection. Induced...
Plant defense inducers that mimic functions of the plant immune hormone salicylic acid (SA) often affect growth. Although benzothiadiazole (BTH), a synthetic analog SA, has been widely used to protect crops from diseases by inducing responses, we recently demonstrated but not BTH, confers resistance against Rhizoctonia solani, causal agent sheath blight disease, in Brachypodium distachyon. Here, BTH compromised Bd3-1 and Gaz4, two blight-resistant accessions B. distachyon, which activate...
Seedling rot, caused by the bacterial pathogen Burkholderia glumae, is a major disease of rice. It originates from pathogen-contaminated seeds and thus mainly controlled pesticide treatments seeds. We previously demonstrated that seed-borne bacteria rice may be useful sustainable alternative to pesticides manage seedling but they are limited in terms variety. Here, we report another bacterium, Pantoea dispersa BB1, protects B. glumae. screened 72 isolates three genetically different...
Purpose We considered the possibility of reducing industrial waste by fabricating and reusing dental models prepared using a fused deposition modeling (FDM) 3D printer polylactic acid (PLA) filaments. The purpose this study was to verify accuracy fabricated FDM PLA.
Plants activate defense responses through the recognition of microbe-associated molecular patterns (MAMPs). Recently, several pattern-recognition receptors (PRRs) have been identified in plants, paving way for manipulating MAMP signaling. CEBiP is a receptor chitin elicitor (CE) rice plasma membrane and XA21 member receptor-like protein kinase (RLK) family that confers disease resistance to bacterial leaf blight expressing sulfated Ax21. To improve blast, most serious fungal rice, we aimed...
We have purified and characterized an oligoxylosyl transfer enzyme (OxtA) from Bacillus sp. strain KT12. In the present study, a N-terminally His-tagged recombinant form of enzyme, OxtA(H)E, was overproduced in Escherichia coli applied to reaction with xylan hydroquinone produce 4-hydroxyphenyl β-d-oligoxylosides, β-(Xyl)n-HQ (n=1–4), by one step reaction. The obtained inhibited mushroom tyrosinase, which catalyzes oxidation l-DOPA quinine, IC50 values β-Xyl-HQ, β-(Xyl)2-HQ, β-(Xyl)3-HQ,...
Rhizoctonia solani is a necrotrophic plant pathogen with wide host range. R. species complex consisting of thirteen anastomosis groups (AGs) defined by compatibility hyphal fusion reaction and subgroups based on cultural morphology. The relationship between such classifications specificity remains elusive. Here, we investigated the pathogenicity seventeen isolates (AG-1 to 7) in Japan towards Arabidopsis thaliana using leaf soil inoculations. tested AGs, except AG-3 AG-6, induced symptoms...
Rhizoctonia solani is a necrotrophic phytopathogen belonging to basidiomycetes. It causes rice sheath blight which inflicts serious damage in production. The infection strategy of this pathogen remains unclear. We previously demonstrated that salicylic acid-induced immunity could block R. AG-1 IA both and Brachypodium distachyon. may undergo biotrophic process using effector proteins suppress host before stage. To identify genes expressed at the early process, here we developed an...
Salicylic acid (SA) is known to be involved in the immunity against
Plants protect themselves from microorganisms by inducing pattern-triggered immunity (PTI) via recognizing microbe-associated molecular patterns (MAMPs), conserved across many microbes. Although the MAMP perception mechanism and initial events during PTI have been well-characterized, knowledge of transcriptomic changes in plants, especially monocots, is limited intermediate terminal stages PTI. Here, we report a time-series high-resolution RNA-sequencing (RNA-seq) analysis leaf disks...
Rice seed-borne diseases caused by the bacterial pathogens Burkholderia glumae and B . plantarii pose a major threat to rice production worldwide. To manage these in sustainable manner, biocontrol strategy is crucial. In this study, we showed that gladioli NB6 (NB6), nonpathogenic bacterium, strongly protects from infection above-mentioned pathogens. was isolated indica cultivar Nona Bokra seedlings, which possesses genetic resistance We discovered cell suspensions of its culture filtrate...