A5004314546- interferon and immune responses
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- Viral Infections and Outbreaks Research
- Mycobacterium research and diagnosis
- Microbial infections and disease research
- Molecular Sensors and Ion Detection
- DNA Repair Mechanisms
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Luminescence and Fluorescent Materials
- Viral Infections and Vectors
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Cytomegalovirus and herpesvirus research
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2025
Fudan University
2022-2025
Huashan Hospital
2025
Academy of Medical Sciences
2025
Shanghai Xuhui Central Hospital
2023
Shanghai Medical College of Fudan University
2021
State Key Laboratory of Medicinal Chemical Biology
2017
Nankai University
2017
Ten-eleven translocation (TET) enzymes oxidize 5-methylcytosine (mC) in DNA, contributing to the regulation of gene transcription. Diverse mutations TET2 are frequently found various blood cancers, yet full scope their functional consequences has been unexplored. Here, we report that a subset identified leukemia patients alter substrate specificity from acting on mC thymine. This neomorphic activity results substitutions at key residues involved interactions with base, including Asn1387 and...
Abstract Somatic loss-of-function mutations of the dioxygenase Ten-eleven translocation-2 (TET2) occur frequently in individuals with clonal hematopoiesis (CH) and acute myeloid leukemia (AML). These common hematopoietic disorders can be recapitulated mouse models. However, underlying mechanisms by which deficiency TET2 promotes these remain unclear. Here we show that cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS)-stimulator interferon genes (STING) pathway is...
Somatic mutations in DNA methyltransferase 3 A (DNMT3A) are frequently observed patients with hematological malignancies. Hematopoietic stem/progenitor cells (HSPCs) mutated DNMT3A demonstrate increased self-renewal activity and skewed lineage differentiation. However, the molecular mechanisms underlying these changes remain largely unexplored. In this study, we show that Dnmt3a loss leads to upregulation of endogenous retroviruses (ERVs) HSPCs, subsequently activating cGAS-STING pathway...
As an aberrant base in DNA, uracil is generated by either deoxyuridine (dU) misincorporation or cytosine deamination, and involved multiple physiological pathological processes. Genome-wide profiles of are important for study these Current methods whole-genome mapping all rely on uracil-DNA N-glycosylase (UNG) limited resolution, specificity, and/or sensitivity. Here, we developed a UdgX cross-linking polymerase stalling sequencing ("Ucaps-seq") method to detect dU at single-nucleotide...
A near-infrared fluorescent turn-on probe has been reported for specific HER2 imaging and synergistic enhancement of anticancer activity doxorubicin.
Abstract Somatic loss-of-function mutations of the dioxygenase Ten-eleven translocation-2 (TET2) occur frequently in individuals with clonal hematopoiesis (CH) and acute myeloid leukemia (AML). These common hematopoietic disorders can be recapitulated mouse models. However, underlying mechanisms by which deficiency TET2 promotes these remain largely unknown. Here we show that cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS)-stimulator interferon genes (STING) pathway is...
ABSTRACT As an aberrant base in DNA, uracil is generated by dUMP misincorporation or cytosine deamination, and involved multiple physiological pathological processes. Current methods for whole-genome mapping of all rely on uracil-DNA N -glycosylase (UNG) are limited resolution specificity. Here, we present a UNG-independent Single-Nucleotide Uracil Sequencing (SNU-seq) method utilizing the UdgX protein which specifically excises forms covalent bond with resulting deoxyribose. SNU-seq was...