A5058121881- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Head and Neck Cancer Studies
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Migration, Health and Trauma
- Inflammatory Biomarkers in Disease Prognosis
- Ferroptosis and cancer prognosis
- Immune cells in cancer
- Immune responses and vaccinations
- Pregnancy and preeclampsia studies
- Esophageal Cancer Research and Treatment
- Down syndrome and intellectual disability research
- Endometriosis Research and Treatment
- Health and Conflict Studies
- Circular RNAs in diseases
- Colorectal and Anal Carcinomas
- Cancer-related molecular mechanisms research
- Prenatal Screening and Diagnostics
Dartmouth College
2023-2025
Dartmouth–Hitchcock Medical Center
2023-2024
Abstract Aging is a significant risk factor for various human disorders, and DNA methylation clocks have emerged as powerful tools estimating biological age predicting health‐related outcomes. Methylation data from blood has been focus of more recently developed clocks. However, the impact immune cell composition on epigenetic acceleration (EAA) remains unclear only some incorporate partial type information when analyzing EAA. We investigated associations 12 types measured by cell‐type...
Menstrual effluent cell profiles have potential as noninvasive biomarkers of female reproductive and gynecological health disease. We used DNA methylation-based type deconvolution (methylation cytometry) to identify in self-collected menstrual effluent. During the second day their cycle, healthy participants collected using a vaginal swab, cup, pad. Immune proportions were highest cup samples, epithelial cells swab samples. Our work demonstrates feasibility utility profiling population-level...
Abstract Background: Head and neck squamous cell carcinomas (HNSCC) can occur in the oral cavity, pharynx, larynx, paranasal sinuses, nasal cavity. Immune checkpoint inhibitors (ICI) have improved survival multiple cancer types, including recurrent/metastatic (R/M) HNSCC. However, majority of patients with R/M HNSCC did not achieve objective response ICI clinical trials. Although tumor tissue DNA methylation has been proposed as a potential biomarker for response, less is known about blood...
Down syndrome (DS) is a genetic disorder caused by an extra copy of chromosome 21 that presents developmental dysfunction and intellectual disability. To better understand the cellular changes associated with DS, we investigated cell composition in blood, brain, buccal swab samples from DS patients controls using DNA methylation-based cell-type deconvolution. We used genome-scale methylation data Illumina HumanMethylation450k HumanMethylationEPIC arrays to profile trace fetal lineage cells...
Aim: This study addresses the challenge of predicting response head and neck squamous cell carcinoma (HNSCC) patients to immunotherapy. Methods: Using DNA methylation cytometry, we analyzed immune profiles six HNSCC who showed a positive immunotherapy over year without disease progression. Results: There was an initial increase in CD8 T memory cells natural killer during first four cycles immunotherapy, which then returned baseline levels after year. Baseline were lower responders but became...
Abstract Menstrual effluent cell profiles have potential as noninvasive biomarkers of female reproductive and gynecological health disease. We used DNA methylation-based type deconvolution (methylation cytometry) to identify in self-collected menstrual effluent. During the second day their cycle healthy participants collected using a vaginal swab, cup, pad. Immune proportions were highest cup samples, epithelial cells swab samples. Our work demonstrates feasibility utility profiling...
Abstract We investigated associations between prenatal genocidal trauma, including maternal rape, and postnatal adverse childhood experiences (ACEs) on DNA methylation of genes associated with the stress response. In a comparative cross-sectional study 91 Rwandan young adults, categorized by exposure to genocide without unexposed controls, we analyzed from dried blood spots assessed ACEs mental health at age 24. Prenatal rape was changes in BDNF SLC6A4 , association attenuated exposure....
Prenatal exposure to trauma, including genocide and maternal rape, adverse childhood experiences (ACEs), are associated with lifespan reduction. We evaluated whether prenatal or genocidal ACEs among individuals conceived during the 1994 against Tutsi in Rwanda were differences age acceleration three first-generation (Horvath, Hannum, PhenoAge) four second-generation epigenetic aging clocks (GrimAge, DunedinPace, YingDamAge, YingAdaptAge), given association between biological mortality. No...
We investigated associations between prenatal genocidal trauma, including maternal rape, and postnatal adverse childhood experiences (ACEs) on DNA methylation of genes associated with the stress response. In a comparative cross-sectional study 91 Rwandan young adults, categorized by exposure to genocide without unexposed controls, we analyzed from dried blood spots assessed ACEs depression anxiety symptoms at age 24. Prenatal rape was changes in BDNF SLC6A4, association attenuated after ACE...
Abstract Background: Standard-of-care anti-PD-1 therapy does not provide long-lasting benefits for most patients with recurrent/metastatic (R/M) head and neck squamous cell carcinoma (HNSCC). A current biomarker of potential response, PD-ligand-1 combined proportion score, has limitations due to the heterogeneity availability tumor samples. The development peripheral blood-based biomarkers offers a contemporary noninvasive sample immunotherapy response. DNA methylation-based immune...
<h3>Background</h3> Immune checkpoint inhibitors (ICIs) are approved to treat patients with recurrent/metastatic head and neck squamous cell carcinoma (HNSCC). However, currently biomarkers limited due the heterogeneity availability of tumor samples. The development peripheral offers an alternative noninvasive approach assess immunotherapy response. DNA methylation-based immune deconvolution provides opportunities for developing blood-based predict response outcomes in HNSCC....