A5037629507- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Acute Ischemic Stroke Management
- Monoclonal and Polyclonal Antibodies Research
- Intracerebral and Subarachnoid Hemorrhage Research
- Psychosomatic Disorders and Their Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Mental Health and Psychiatry
- Climate Change and Health Impacts
- Chronic Disease Management Strategies
- Air Quality and Health Impacts
- Immunodeficiency and Autoimmune Disorders
- Global Health Care Issues
- Carbohydrate Chemistry and Synthesis
- Parvovirus B19 Infection Studies
- Empathy and Medical Education
- Connective tissue disorders research
- Microbial infections and disease research
- Blood properties and coagulation
- Cerebrovascular and genetic disorders
- Interprofessional Education and Collaboration
- Stroke Rehabilitation and Recovery
- Sustainability and Climate Change Governance
- Healthcare innovation and challenges
East London NHS Foundation Trust
2024
Cambridge University Hospitals NHS Foundation Trust
2024
University of Bedfordshire
2024
University of Washington
2022-2023
University of Denver
2020-2021
Campbell Collaboration
2021
University of Bristol
2016
Bristol Institute for Transfusion Sciences
1997-2016
National Health Service
2016
NHS Blood and Transplant
2016
Cord blood stem cells are an attractive starting source for the production of red in vitro therapy because additional expansion potential compared with adult peripheral progenitors and cord banks usually being more representative national populations than donors. Consequently, it is important to establish how similar RBCs cells. In this study, we used multiplex tandem mass tag labeling combined nano-LC-MS/MS compare proteome reticulocytes. 2838 unique proteins were identified, providing most...
Abstract Background Functional somatic symptoms (FFS) and bodily distress disorders are highly prevalent across all medical settings. Services for these patients dispersed the health care system with minimal conceptual operational integration, do not currently access therapeutic offers in significant numbers due to a mismatch between their professionals’ understanding of nature symptoms. New service models urgently needed address patients’ needs align advances aetiological evidence...
Summary Tn polyagglutination results from inactivating mutations in C1GALT1C1 , an X‐borne gene encoding a core 1 β3‐galactosyltransferase‐specific molecular chaperone (cosmc) required for the functioning of T‐synthase (β 1,3‐galactosyltransferase), glycosyltransferase essential correct biosynthesis O ‐glycans. This study found novel (Glu152Lys, Ser193Pro and Met1Ile) coding sequence three positive individuals complete lack cDNA expression was observed additional individual. In addition,...
Introduction Goal-setting is recommended for patients with multimorbidity, but there little evidence to support its use in general practice. Objective To assess the feasibility of goal-setting before undertaking a definitive trial. Design and setting Cluster-randomised controlled trial compared control six practices. Participants Adults two or more long term health conditions at risk unplanned hospital admission. Interventions General practitioners (GPs) underwent training were asked...
Dantu, a previously undescribed low-incidence red cell antigen, is inherited as Mendelian dominant character. The Dantu antigen associated with very weak s protease resistant N and either or no U antigen. Two of the propositi had been shown to have an unusual hybrid MNSs sialoglycoprotein, it probably this which carries these N, antigens well A study family one propositus suggests, by conventional genetics, that not controlled locus; possible explanation given. Several examples anti-Dantu...
The null phenotype of the Lutheran blood group system, Lu(null) or Lu(a-b-), is characterized by lack all system antigens. It can arise from three genetic backgrounds: recessive, dominant, X-linked. recessive type appears to result homozygosity for an inactive LU gene.Three unrelated individuals were assessed standard serologic tests. All exons gene directly sequenced amplified genomic DNA. validity observed mutations within was confirmed use either restriction enzymes allele-specific...
Background and Objectives: We have analysed the appearance disappearance of cell surface markers during crythropoiesis, in vitro . Materials Methods: CD34 + haemopoietic progenitor cells were isolated from umbilical cord blood cultured by three different methods, all presence erythropoietin. The methods included two one‐stage techniques, one serum‐free, other with serum present, scrum‐present two‐stage method. Results: on differentiating erythroid varied slightly sample to sample, but...
BACKGROUND: The KEL2/KEL1 (k/K) blood group polymorphism represents 578C>T in the KEL gene and Thr193Met Kell glycoprotein. Anti‐KEL1 can cause severe hemolytic disease of fetus newborn. Molecular genotyping for * 1 is routinely used assessing whether a at risk. Red cells (RBCs) from KEL:1 donor (D1) were found to have abnormal KEL1 expression during evaluation anti‐KEL1 reagents. STUDY DESIGN AND METHODS: methods, including exon 6 direct sequencing, applied. cDNA D1 was sequenced. Flow...
BACKGROUND: Lutheran is a complex blood group system consisting of 18 identified antigens. There are four pairs allelic antigens, whereas others independently expressed antigens high frequency. carried by the glycoproteins, which product single gene LU . STUDY DESIGN AND METHODS: Genomic DNA from 21 individuals 12 phenotypes was used for PCR amplification selected exons that were directly sequenced and compared to control common phenotype. RESULTS: mostly caused single‐nucleotide...
BACKGROUND: The JAHK antigen was first described in 1995 as a low‐frequency red blood cell antigen. Family studies confirmed the association of with rare r G phenotype Rh group system, which is associated weak expression C and e, but normal expression. allocated number RH53. serologic findings indicated location on RhCE protein, although molecular basis for has not been known. STUDY DESIGN AND METHODS: RHCE gene eight persons from three unrelated families analyzed by exon amplification...
The glycophorin (GP) molecule associated with the GP.Dane phenotype is a GP(A-B-A) hybrid that contains some amino acids encoded by Pseudoexon 3 of GYPB and Asn(45) GPA carries low-prevalence MNS antigens DANE Mur. Serum from woman English ancestry contained an immunoglobulin M alloantibody to high-prevalence antigen, purpose this study was identify molecular basis her phenotype.Hemagglutination, Western blotting, DNA analyses were performed standard methods.Tests proband's RBCs monoclonal...
Abstract. For some time, anomalous serological reactions have been observed when the same anti‐Sw a sera are tested against red cells from different individuals reported as Sw(a+). A comparative collaborative study using collection of Sw(a+) and was undertaken by 4 reference laboratories, it found that Sw represents heterogeneous group antigens can be subdivided into two categories. Both categories, 700:41 700:‐41, were shown to inherited.
Purpose With care clusters an established framework for mental health services it is timely to consider how use them deliver high quality, evidence based that socially inclusive and recovery oriented. This paper aims describe conceptual thinking about these issues, specifically in relation the challenges balances inherent packages approach. It seeks work develop internet based, high‐level description of such each cluster. Design/methodology/approach The background project described, along...
<title>Abstract</title> Background: Functional somatic symptoms and bodily distress disorders are highly prevalent across all medical settings. Services for these patients dispersed the health care system with minimal conceptual operational integration do not currently access therapeutic offers in significant numbers due to a mismatch between their professionals’ understanding of nature symptoms. New service models urgently required address patients’ needs align advances aetiological...
Mi.IX is a new phenotype in the Miltenberger series of MNS blood group system with frequency 0.43% Denmark. red cells are Mur+ but do not express any other established determinants. They react antibody, anti-DANE, which defines determinant present on phenotypes. Four propositi have been found. Their families show that Mi^lx inherited MS complex (lod score 3.69 at Θ = 0.00) produces trypsin-resistant M antigen. DANE has allotted ISBT number 002032 (MNS32). Serological and immunochemical...
For some time, anomalous serological reactions have been observed when the same anti-Sw^a sera are tested against red cells from different individuals reported as Sw(a+). A comparative collaborative study using collection of Sw(a+) and anti-Swa was undertaken by 4 reference laboratories, it found that Sw^a represents a heterogeneous group antigens can be subdivided into two categories. Both categories, 700:41 700:-41, were shown to inherited.
Red cells carrying the low-frequency MNS antigen M^g reacted with only example of anti-DANE, an antibody which had previously defined GP.Dane (Mi.IX) phenotype. Furthermore, + original anti-Mur (serum Mrs. Murrell), but none 14 other anti-Mur. Therefore, carry both DANE and atypical Mur antigen. Immunoblotting membranes from anti-M, eluates prepared anti-M^g Murrell's serum demonstrated a glycophorin A (GPA) molecule whose mobility was increased by apparent M(r) about 3,000 presumably due to...