A5070932477- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Viral Infections and Immunology Research
- Vibrio bacteria research studies
- Nicotinic Acetylcholine Receptors Study
- RNA and protein synthesis mechanisms
- Ion channel regulation and function
- RNA modifications and cancer
- DNA Repair Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Cardiac electrophysiology and arrhythmias
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Immune Cell Function and Interaction
- Insect and Pesticide Research
- CRISPR and Genetic Engineering
Skolkovo Institute of Science and Technology
2023-2025
Institute of Molecular and Cell Biology
2024
Lomonosov Moscow State University
2020
Viruses compete with each other for limited cellular resources, and some viruses deliver defense mechanisms that protect the host from competing genetic parasites. PARIS is a system, often encoded in viral genomes, composed of 53 kDa ABC ATPase (AriA) 35 TOPRIM nuclease (AriB). Here we show AriA AriB assemble into 425 supramolecular immune complex. We use cryo-EM to determine structure this complex which explains how six molecules propeller-shaped scaffold coordinates three subunits AriB....
Viruses compete with each other for limited cellular resources, and some deliver defence mechanisms that protect the host from competing genetic parasites
Abstract Anti-phage systems of the BREX (BacteRiophage EXclusion) superfamily rely on site-specific epigenetic DNA methylation to discriminate between host and invading DNA. We demonstrate that in Type I systems, defense require site binding by BrxX (PglX) methyltransferase employing S-adenosyl methionine as a cofactor. determined 2.2-Å cryoEM structure Escherichia coli bound target dsDNA revealing molecular details recognition. Structure-guided engineering expands its specificity...
ArdB, ArdA, and Ocr proteins inhibit the endonuclease activity of type I restriction-modification enzymes (RMI). In this study, we evaluated ability to different subtypes Escherichia coli RMI systems (IA, IB, IC) as well two Bacillus licheniformis systems. Furthermore explored, antirestriction against a III system (RMIII) EcoPI BREX. We found that DNA-mimic proteins, ArdA exhibit inhibition activity, depending on which RM tested. This effect might be linked DNA mimicry nature these proteins....
Bacteriophage BF23 is a close relative of phage T5, prototypical Tequintavirus that infects Escherichia coli. was isolated in the middle XXth century and extensively studied as model object. Like carries long ∼9.7 kb terminal repeats, injects its genome into infected cell two-stage process, multiple specific nicks double-stranded genomic DNA. The two phages rely on different host secondary receptors-FhuA (T5) BtuB (BF23). Only short fragments genome, including region encoding receptor...
ABSTRACT Anti-phage systems of the BREX (BacteRiophage EXclusion) superfamily rely on epigenetic DNA methylation to discriminate between host and invading DNA, but their mechanism protection remains enigmatic. We demonstrate that in Type I systems, both defense are based site-specific recognition by BrxX (PglX) methyltransferase require S-adenosyl methionine cofactor. present a 2.2-Å cryoEM structure Escherichia coli bound target dsDNA, which reveals molecular details paves way for rational...
Abstract Bacterial defence systems are tightly regulated to avoid autoimmunity. In Type I restriction–modification (R–M) systems, a specific mechanism called restriction alleviation (RA) controls the activity of module. case Escherichia coli R–M system EcoKI, RA proceeds through ClpXP-mediated proteolysis complexes bound non-methylated sites that appear after replication or reparation host DNA. Here, we show is also induced in presence plasmids carrying EcoKI recognition sites, phenomenon...
Using
An abstract is not available for this content so a preview has been provided. As you have access to content, full PDF via the ‘Save PDF’ action button.
In the laboratory of metagenome analysis there were obtained Escherichia coli BW25113 mutants resistant to infection with T7 phage and possessing a mucoid phenotype. Tasks set in carrying out whole genome sequencing their further bioinformatic processing on basis order determine mechanism acquiring resistance bacteriophages. Bioinformatic included searching variants for strains under investigation by Bowtie2 SNP calling software, prediction importance IGV-browser, predicting functions use...