A5049533478- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Advanced biosensing and bioanalysis techniques
- Insect symbiosis and bacterial influences
- Innovation and Socioeconomic Development
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Cytomegalovirus and herpesvirus research
- International Arbitration and Investment Law
- Analytical Chemistry and Sensors
- Escherichia coli research studies
- International Maritime Law Issues
- Venomous Animal Envenomation and Studies
- Genetics, Bioinformatics, and Biomedical Research
- DNA and Nucleic Acid Chemistry
- Plant Disease Resistance and Genetics
- Mosquito-borne diseases and control
University of Toronto
2016-2025
Washington University in St. Louis
1999-2025
Glasgow Caledonian University
2022
Canada Research Chairs
2000-2018
Argonne National Laboratory
2005
Ontario Institute for Cancer Research
2003-2005
University of Chicago
2005
University of New Brunswick
2002
Pfizer (United States)
1999
Intel (United States)
1997
CRISPR-Cas systems are one of the most widespread phage resistance mechanisms in prokaryotes. Our lab recently identified first examples phage-borne anti-CRISPR genes that encode protein inhibitors type I-F system Pseudomonas aeruginosa. A key question arising from this work was whether there other types genes. In current work, we address by demonstrating some same phages carrying also possess mediate inhibition I-E P. We have discovered four distinct families these These do not inhibit...
Recent years have seen the publication of both empirical and theoretical relationships predicting rates with which proteins fold. Our ability to test refine these has been limited, however, by a variety difficulties associated comparison folding unfolding rates, thermodynamics, structure across diverse sets proteins. These include wide, potentially confounding range experimental conditions methods employed date difficulty obtaining correct complete sequence structural details for...
Significance In double-stranded DNA tailed bacteriophages and herpes viruses, genomes are packaged into a preformed protein shell called the “prohead.” Terminase enzymes responsible for cleaving replicated concatemeric single genome units, mediating packaging of these prohead. We have discovered that member very large family nuclease-associated proteins “HNH proteins” is crucial component terminase reaction Escherichia coli phage HK97. Bioinformatic studies indicate HNH associated with...
ABSTRACT A phage moron is a DNA element inserted between pair of genes in one genome that are adjacent other related genomes. Phage morons commonly found within genomes, and number cases, they have been shown to mediate phenotypic changes the bacterial host. The temperate HK97 encodes element, gp15, its tail morphogenesis region absent most closely phages. We show gp15 actively expressed from prophage responsible for providing host cell with resistance infection by phages HK75, independent...
Phages play critical roles in the spread of virulence factors and control bacterial populations through their predation bacteria. An essential step phage lifecycle is genome entry, where infecting must productively interact with components cell envelope order to transmit its out viral particle into host cytoplasm. In this study, we characterize process for Escherichia coli HK97. We have discovered that HK97 injection requires activities inner membrane glucose transporter protein, PtsG,...
As one of their countermeasures against CRISPR-Cas immunity, bacteriophages have evolved natural inhibitors known as anti-CRISPR (Acr) proteins. Despite the existence such examples for type II systems, we currently know relatively little about breadth Cas9 inhibitors, and most direct targets are uncharacterized. In this work identify two new II-C anti-CRISPRs cognate orthologs, validate functionality in vitro bacteria, define inhibitory spectrum a panel demonstrate that they act before DNA...
Pf bacteriophage (phage) are filamentous viruses that infect Pseudomonas aeruginosa and enhance its virulence potential. virions can lyse kill P. through superinfection, which occurs when an already infected cell is by the same or similar phage.