A5048060547- 3D Printing in Biomedical Research
- Hydrogels: synthesis, properties, applications
- Monoclonal and Polyclonal Antibodies Research
- Polymer Surface Interaction Studies
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Nanoplatforms for cancer theranostics
- Nanofabrication and Lithography Techniques
- Photochromic and Fluorescence Chemistry
- Graphene and Nanomaterials Applications
- Nonlinear Optical Materials Studies
- Angiogenesis and VEGF in Cancer
- Alzheimer's disease research and treatments
- Bone Tissue Engineering Materials
- Cellular Mechanics and Interactions
- Marine Biology and Environmental Chemistry
- Biotin and Related Studies
- Advanced Biosensing Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Proteoglycans and glycosaminoglycans research
- Parkinson's Disease Mechanisms and Treatments
- Advanced Polymer Synthesis and Characterization
- Silk-based biomaterials and applications
- Advanced Sensor and Energy Harvesting Materials
- Neurogenesis and neuroplasticity mechanisms
McMaster University
2015-2024
Boston Children's Hospital
2013-2024
Harvard University
2013-2024
Hamilton Health Sciences
2020
University of Toronto
2008-2015
Massachusetts Institute of Technology
2013-2015
Muscular Dystrophy Canada
2015
McGill University
2009
National Measurement Laboratory
1954
We report plasmonic gold nanoshells and nanorods coated with reduced graphene oxide that produce an enhanced photothermal effect when stimulated by near-infrared (NIR) light. Electrostatic interactions between nanosized nanoparticles followed in situ chemical reduction generated oxide-coated nanoparticles; the coating was demonstrated using Raman HR-TEM. Reduced showed compared to noncoated or nonreduced nanoparticles. killed cells more rapidly than did
High-efficiency upconverted light would be a desirable stimulus for triggered drug delivery. Here we present general strategy to achieve photoreactions based on triplet-triplet annihilation upconversion (TTA-UC) and Förster resonance energy transfer (FRET). We designed PLA-PEG micellar nanoparticles containing in their cores hydrophobic photosensitizer annihilator molecules which, when stimulated with green light, undergo TTA-UC. The was then transferred by FRET photocleavable group (DEACM),...
Primary endothelial cells are guided in an agarose hydrogel scaffold that is chemically patterned with immobilized concentration gradient of VEGF165 using multiphoton laser patterning. It particularly compelling that, this 3D hydrogel, differentiate to tip and stalk cells, having the morphology observed vivo. Detailed facts importance specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by...
Atomic resolution map of the soluble amyloid beta assembly (Aβ<sub>n</sub>) “toxic surfaces” that facilitate early pathogenic events in Alzheimer's disease (AD).
Terthienobenzene (TTB, 6) was prepared through a new, high yield route along with pi-extended derivatives 10 and 11. Electropolymerization of tris-EDOT derivative 11 results in highly stable cross-linked conjugated polymer that shows polaron confinement between the TTB units as confirmed by UV-vis-NIR spectroelectrochemistry EPR.
The ability to create three-dimensional micropatterns within polymeric materials is applicable in a wide number of fields, from photonic bandgaps tissue engineering. We are particularly interested chemical patterning soft with view towards their use regenerative medicine. To this end, we created amines an agarose hydrogel using two-photon patterning. Agarose was first modified caged amines, derivative 6-bromo-7-hydroxycoumarin, which upon excitation cleaved the coumarin molecule thereby...
The ability to create three-dimensional biochemical environments that mimic those in vivo is valuable for the elucidation of fundamental biological phenomena and pathways. To this end, we designed a system which proteins can be photochemically patterned three dimensions within hydrogels under physiological conditions. Fibroblast growth factor-2 (FGF2) was immobilized agarose were modified with two-photon labile 6-bromo-7-hydroxycoumarin-protected thiols. Two different methods developed FGF2...
Increasing the polymer content on biosensors is important to improve sensor function by altering surface properties and increasing number of capture sites for analytes. Grafting-to methods are often employed but may be limited insufficient immobilization. Herein, we have utilized Graft-then-Shrink (GtS) simultaneously increase grafting-to surfaces produce low-cost, local plasmon resonance (LSPR) Au biosensors. The were incorporated within microwell plates, where translocation materials...
Abstract With increased clinical use of antibodies, long‐term delivery strategies are needed to decrease injection frequency and improve health outcomes. A three‐component drug‐delivery system was developed for competitive affinity release a streptavidin–antibody conjugate from agarose–desthiobiotin hydrogels via controlled dissolution sparingly soluble biotin derivatives. The antibody localized in the hydrogel through streptavidin–desthiobiotin complexation. Dissolution derivatives disrupts...
Alpha synuclein (αS) oligomers are a key component of Lewy bodies implicated in Parkinson's disease (PD). Although primarily intracellular, extracellular αS exocytosed from neurons also contributes to PD pathogenesis through prion-like transmission mechanism. Here, we show at progressive degrees resolution that the most abundantly expressed protein, human serum albumin (HSA), inhibits oligomer (αSn) toxicity three-pronged First, endogenous HSA targets αSn with sub-μM affinity via...
Chemical immunotherapeutic strategies including Antibody Recruiting Molecules (ARMs - bivalent small molecules containing an antibody-binding domain (ABD) and a target-binding (TBD)) direct immune-mediated clearance of diseased cells. Anti-cancer ARM function relies on high tumor antigen valency, limiting against lower expressing tumors. To address this limitation, we report tunable multivalent immune recruitment (MIR) platform to amplify/stabilize antibody cells with valencies. An initial...
Bispecific antibodies (bsAbs) are emerging immune-therapeutics, and many formats exist that differ considerably in structure. However, little systematic data about how the spatial organization of their components influences activity, requiring innovative approaches combining empirical quantitative frameworks. This study presents a modular DNA nanotechnology platform to generate numerous bsAbs with surrogate geometries span structural features BiTE, IgG-like, IgG-conjugate platforms screen...
Poly(carboxybetaine) (pCB) hydrogels do not elicit a foreign body response due to their low-fouling properties, making them ideal implantable materials for in vivo drug and cell delivery. Current reported pCB are cross-linked using cytotoxic UV-initiated radical polymerization limiting clinical translation. For translation, we require situ biorthogonal cross-linking of that both low-swelling limit nonspecific interactions minimize tissue damage, respectively. To this end, synthesized...
Abstract With increased clinical use of antibodies, long‐term delivery strategies are needed to decrease injection frequency and improve health outcomes. A three‐component drug‐delivery system was developed for competitive affinity release a streptavidin–antibody conjugate from agarose–desthiobiotin hydrogels via controlled dissolution sparingly soluble biotin derivatives. The antibody localized in the hydrogel through streptavidin–desthiobiotin complexation. Dissolution derivatives disrupts...
Current methods to tune release rates of therapeutic antibodies (Abs) for local delivery are complex and routinely require bioconjugations that may reduce Ab bioactivity. To rapidly profiles bioactive Abs, we developed a biophysical interaction system within neutravidin modified poly(carboxybetaine) hydrogel (pCB-NT) tunes desthiobiotinylated Abs (D-Abs) using constant D-Ab combination. Herein, delivered bevacizumab (D-Bv), recombinant humanized monoclonal IgG1 antiangiogenic cancer...
Methods to reversibly control the chemical environment of hydrogels have application in three-dimensional cell culture study proliferation, migration and differentiation environments more representative vivo environments. Herein, we developed a method temporally agarose through non-covalent attachment peptide motifs. Streptavidin-GRGDS conjugates were immobilized desthiobiotin-modified desthiobiotin-streptavidin interaction (KD 10-11 M). was then displaced from gel by addition biotin, which...