A5100401552- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Advanced biosensing and bioanalysis techniques
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- RNA regulation and disease
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Genetics, Aging, and Longevity in Model Organisms
- Phytochemicals and Antioxidant Activities
- Cytomegalovirus and herpesvirus research
- Adipokines, Inflammation, and Metabolic Diseases
- Bacterial biofilms and quorum sensing
- Diphtheria, Corynebacterium, and Tetanus
- Peptidase Inhibition and Analysis
- Electrocatalysts for Energy Conversion
- Inhalation and Respiratory Drug Delivery
- Wound Healing and Treatments
- Antimicrobial Resistance in Staphylococcus
University of Massachusetts Chan Medical School
2016-2020
Catholic University of Korea
2013-2014
Korea University
2007-2013
Bucheon St. Mary's Hospital
2013
Yonsei University
2013
Kyoto University
2009
As one of their countermeasures against CRISPR-Cas immunity, bacteriophages have evolved natural inhibitors known as anti-CRISPR (Acr) proteins. Despite the existence such examples for type II systems, we currently know relatively little about breadth Cas9 inhibitors, and most direct targets are uncharacterized. In this work identify two new II-C anti-CRISPRs cognate orthologs, validate functionality in vitro bacteria, define inhibitory spectrum a panel demonstrate that they act before DNA...
Abstract CRISPR-associated (Cas) DNA-endonucleases are remarkably effective tools for genome engineering, but have limited target ranges due to their protospacer adjacent motif (PAM) requirements. We demonstrate a critical expansion of the targetable sequence space type II-A enzyme through identification natural 5 $$^{\prime}$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msup><mml:mrow/><mml:mrow><mml:mo>′</mml:mo></mml:mrow></mml:msup></mml:math> -NAAN-3 PAM preference...
CRISPR-Cas systems are bacterial adaptive immune pathways that have revolutionized biotechnology and biomedical applications. Despite the potential for human therapeutic development, there many hurdles must be overcome before its use in clinical settings. Some safety concerns arise from editing activity unintended cell types or tissues upon vivo delivery (e.g., by adeno-associated virus (AAV) vectors). Although tissue-specific promoters serotypes with tissue tropisms can used, suitably...
While genome editing has been revolutionized by the advent of CRISPR-based nucleases, difficulties in achieving efficient, nuclease-mediated, homology-directed repair (HDR) still limit many applications. Commonly used DNA donors such as plasmids suffer from low HDR efficiencies cell types, well integration at unintended sites. In contrast, single-stranded (ssDNA) can produce efficient with minimal off-target integration. this study, we describe use ssDNA phage to efficiently and...
CRISPR-Cas9 systems provide powerful tools for genome editing. However, optimal employment of this technology will require control Cas9 activity so that the timing, tissue specificity, and accuracy editing may be precisely modulated. Anti-CRISPR proteins, which are small, naturally occurring inhibitors CRISPR-Cas systems, well suited purpose. A number anti-CRISPR proteins have been shown to potently inhibit subgroups but their maximal inhibitory is generally restricted specific homologs....
Abstract Identification of modules in molecular networks is at the core many current analysis methods biomedical research. However, how well different approaches identify disease-relevant types gene and protein remains poorly understood. We launched “Disease Module DREAM Challenge”, an open competition to comprehensively assess module identification across diverse protein-protein interaction, signaling, co-expression, homology, cancer-gene networks. Predicted network were tested for...
The function of whcB, one the four whiB homologues Corynebacterium glutamicum, was assessed. Cells carrying P180-whcB clone, and thus overexpressing whcB gene, showed retarded growth, probably due to increased sensitivity oxidants, whereas cells lacking (ΔwhcB) did not. However, growth retardation not observed in with additionally whcE deleted. Furthermore, ΔwhcE phenotype, characterized by slow reversed P180-whcB. Like which is also known as a homologue, gene preferentially expressed...
We have reported a transcription profile of an adapted Corynebacterium glutamicum that showed enhanced oxidative stress resistance. To construct artificial stress-resistant strain, gene clusters in the β-ketoadipate pathway, which were up-regulated artificially expressed wild-type C. glutamicum. The strain was unable to grow under 2 mM H2O2 containing minimal medium, while strains expressing pca restored growth same and pcaHGBC expression most significant effect among clusters. expressions...
The Corynebacterium glutamicum WhcA protein, which inhibits the expression of oxidative stress response genes, is known to interact with SpiA protein. In this study, we constructed and analyzed spiA mutant cells goal better understanding function gene. A C. strain overexpressing gene showed retarded cell growth, was caused by an increased sensitivity oxidants. Expression whcA genes repressed oxidant diamide, indicating coordinate regulation dispensability in under stress. spiA-overexpressing...
Abstract While genome editing has been revolutionized by the advent of CRISPR-based nucleases, difficulties in achieving efficient, nuclease-mediated, homology-directed repair (HDR) still limit many applications. Commonly used DNA donors such as plasmids suffer from low HDR efficiencies cell types, well integration at unintended sites. In contrast, single-stranded (ssDNA) can produce efficient with minimal off-target integration. Here, we describe use ssDNA phage to efficiently and...
Abstract CRISPR-Cas systems are bacterial adaptive immune pathways that have revolutionized biotechnology and biomedical applications. Despite the potential for human therapeutic development, there many hurdles must be overcome before its use in clinical settings. Some safety concerns arise from persistent activity of Cas9 after desired editing is complete, or unintended cell types tissues upon vivo delivery [e.g. by adeno-associated viruses (AAV)]. Although tissue-specific promoters...
Respiratory diseases, ranging from minor infections to serious chronic diseases and malignancies, negatively affect the respiratory system are influenced by various environmental factors such as air pollution, occupational hazards, tobacco smoke, well lifestyle, genetic causes, infectious agents. The prevalence severity of requires development advanced models better understand their pathophysiology develop effective treatments. In this context, 3D bioprinting technology is emerging an...
CRISPR-Cas systems are widely used for genome engineering technologies, and in their natural setting, they play crucial roles bacterial archaeal adaptive immunity, protecting against phages other mobile genetic elements. Previously we discovered bacteriophage-encoded Cas9-specific anti-CRISPR (Acr) proteins that serve as countermeasures host immunity by inactivating 1 . We hypothesized the evolutionary advantages conferred anti-CRISPRs would drive widespread occurrence of these nature 2–4...