A5002047599- interferon and immune responses
- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- Islanding Detection in Power Systems
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Microgrid Control and Optimization
- Smart Grid Energy Management
- Plant Reproductive Biology
- NF-κB Signaling Pathways
- Additive Manufacturing and 3D Printing Technologies
- Immune cells in cancer
- Circadian rhythm and melatonin
- Light effects on plants
- Computational Drug Discovery Methods
- RNA modifications and cancer
- Plant Gene Expression Analysis
- Viral Infections and Vectors
- CRISPR and Genetic Engineering
- Innovation and Socioeconomic Development
- Wind Turbine Control Systems
- Protist diversity and phylogeny
- Robotic Mechanisms and Dynamics
Chongqing University
2023
Sixin (China)
2023
The University of Texas Southwestern Medical Center
2017-2022
Molecular Biology Consortium
2022
Chongqing Three Gorges University
2022
University of Toronto
2015
Tsinghua University
2013
The binding of DNA to cyclic GMP-AMP synthase (cGAS) leads the production secondary messenger (cGAMP), which activates innate immune responses. We have shown that cGAS robustly induced formation liquidlike droplets in was activated. disordered and positively charged N terminus enhanced cGAS-DNA phase separation by increasing valencies binding. Long more efficient promoting liquid enzyme activity than short DNA. Moreover, free zinc ions both vitro cells separation. These results demonstrated...
The cyclic guanosine monophosphate (GMP)-adenosine (AMP) synthase (cGAS) detects microbial and self-DNA in the cytosol to activate immune inflammatory programs. cGAS also associates with chromatin, especially after nuclear envelope breakdown when cells enter mitosis. How is regulated during cell cycle transition not clear. Here, we found direct biochemical evidence that activity was selectively suppressed mitosis human lines uncovered two parallel mechanisms underlying this suppression....
The NF-κB essential modulator (NEMO) is a regulatory subunit of the IκB kinase (IKK) complex that phosphorylates inhibitors IκBs. NEMO mediates IKK activation by binding to polyubiquitin chains (polyUb). Here, we show Lys63(K63)-linked or linear polyUb robustly induced formation liquid-like droplets in which was activated. This liquid phase separation driven multivalent interactions between and polyUb. Both ubiquitin-binding (NUB) domain zinc-finger (ZF) mediated contributed cells. Moreover,...
The DNA-sensing enzyme cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) regulates inflammation and immune defense against pathogens malignant cells. Although cGAS has been shown to exert antitumor effects in several mouse models harboring transplanted tumor cell lines, its role tumors arising from endogenous tissues remains unknown. Here, we show that deletion of mice exacerbated chemical-induced colitis colitis-associated colon cancer (CAC). Interestingly, lacking were...
The rapid evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), such as the Omicron variants that are highly transmissible and immune evasive, underscores need to develop therapeutic antibodies with broad neutralizing activities. Here, we used LIBRA-seq technology, which identified SARS-CoV-2–specific B cells via DNA barcoding subsequently single-cell sequenced BCRs, identify an antibody, SW186, could neutralize major SARS-CoV-2 concern, including Beta, Delta, Omicron,...
Significance Vitamin C (also known as ascorbate), an essential nutrient for humans, plays important role in protection against oxidative stress. The ascorbate-dependent oxidoreductase cytochrome b 561 (Cyt ) is a family of highly conserved, multipass transmembrane enzymes found only eukaryotes. Cyt key ascorbate recycling and many other physiological processes, such iron absorption. atomic structure functional mechanism remain unknown. In this study, we report the high-resolution crystal...
Abstract Circadian clock mechanisms have been extensively investigated but the main rate-limiting step that determines circadian period remains unclear. Formation of a stable complex between proteins and CK1 is conserved feature in eukaryotic mechanisms. Here we show FRQ-CK1 interaction, not FRQ stability, correlates with Neurospor mutants. Mutations specifically affect interaction lead to severe alterations period. The has two roles negative feedback loop. First, it phosphorylation profile,...
Cyclic GMP-AMP synthase (cGAS) is a key DNA sensor that detects aberrant cytosolic arising from pathogen invasions or genotoxic stresses. Upon binding to DNA, cGAS activated and catalyzes the synthesis of cyclic (cGAMP), which induces potent antimicrobial antitumor responses. Kaposi sarcoma-associated herpesvirus (KSHV) human tumor virus causes sarcoma several other malignancies. We previously reported KSHV inhibitor (KicGAS) encoded by ORF52, inhibits enzymatic activity, but underlying...
Abstract Background MicroRNAs (miRNAs) and other types of small regulatory RNAs play critical roles in the regulation gene expression at post-transcriptional level plants. Cotton is one most economically important crops, but little known about miRNAs during cotton fiber elongation. Results Here, we combined high-throughput sequencing with computational analysis to identify (sRNAs) related elongation Gossypium hirsutum L. ( G. ). The sequence confirmed 79 miRNA families elongating cells...
Virtual synchronous generators (VSG) are considered a new generation of grid-friendly inverters with the same inertial support characteristics as (SG). However, inertia comes side effects power and frequency response deterioration. The existing VSG oscillation suppression methods have limitations such complex controller parameter tuning, altering characteristics, quality degradation. To address these issues, this paper proposes universal control strategy based on extended virtual (VSG-EVI)....
Coronavirus disease 2019 (COVID-19) caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide as pandemic and enormous global health economical damage. Since December 2019, more than 197 million cases have been reported, causing 4.2 deaths. In the settings of it is an urgent necessity for development effective COVID-19 treatment. While in-vitro screening hundreds antibodies isolated from convalescent patients challenging due to its high cost, use...
When the virtual synchronous generator (VSG) works in a grid with distorted voltages, it is difficult to guarantee quality of its output currents. The existing harmonic suppression schemes are limited various aspects. In this paper, new voltage-current hybrid control (VCHC) scheme proposed. At fundamental frequency, VCHC equivalent voltage follow command from VSG power loop. current at frequencies, which can suppress currents introduced by voltages. Compared methods, greatly improve without...
In recent years, the trapezoidal accelerationd and deceleration algorithm has been widely used in 3D printing stepper motor control research, but is prone to problems such as out-of-step overshoot during repeated fast start stop. order solve above problems, paper focuses on system of screw extrusion type metal platform, proposed a S-trapezoidal acceleration based algorithm, simulated compared with for analysis, finally performed experimental verification. The results show that S-shaped this...