A5044292652- Bacteriophages and microbial interactions
- Proteoglycans and glycosaminoglycans research
- Bacterial biofilms and quorum sensing
- Wound Healing and Treatments
- Glycosylation and Glycoproteins Research
- Cystic Fibrosis Research Advances
- Pancreatic function and diabetes
- Microbial infections and disease research
- Fibroblast Growth Factor Research
- Diabetes and associated disorders
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Genomics and Phylogenetic Studies
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Diabetes Management and Research
- Angiogenesis and VEGF in Cancer
- Platelet Disorders and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pressure Ulcer Prevention and Management
- Neonatal Respiratory Health Research
Stanford University
2016-2025
University of California, San Francisco
2022
Stanford Medicine
2014-2021
Palo Alto University
2014-2021
Stratford University
2020
Baylor College of Medicine
2017
Benaroya Research Institute
2007-2016
Virginia Mason Medical Center
2009-2012
University of Washington
2010-2012
University of Washington Medical Center
2006-2011
Phage subverts immune response Pseudomonas aeruginosa ( Pa ) is a multidrug-resistant Gramnegative bacterium commonly found in health care settings. infections frequently result considerable morbidity and mortality. Sweere et al. that type of temperate filamentous bacteriophage infects integrates into associated with chronic human wound infections. Likewise, wounds mice colonized phage-infected were more severe longer-lasting than those by alone. Immune cell uptake resulted phage RNA...
Abstract Scavenger receptors constitute a large family of proteins that are structurally diverse and participate in wide range biological functions. These expressed predominantly by myeloid cells recognize variety ligands including endogenous modified host-derived molecules microbial pathogens. There currently eight classes scavenger receptors, many which have multiple names, leading to inconsistencies confusion the literature. To address this problem, workshop was organized United States...
Highlights•Filamentous Pf bacteriophage are produced by Pseudomonas aeruginosa•Pf phage interact with host and microbial polymers to assemble higher order structures•Pf increase the viscosity of in cystic fibrosis airway secretions•The Pf-induced liquid crystal biofilm matrix boosts tolerance desiccation antibioticsSummaryBiofilms—communities bacteria encased a polymer-rich matrix—confer ability persist pathologic contexts, such as (CF) airways. How into biofilms is largely unknown. We find...
In humans, multiple genes in the interleukin (IL)-2/IL-2 receptor (IL-2R) pathway are associated with type 1 diabetes. However, no link between IL-2 responsiveness and CD4(+)CD25(+)FOXP3(+) regulatory T-cells (Tregs) has been demonstrated diabetic subjects despite role of these IL-2-dependent cells controlling autoimmunity. Here, we address whether altered impacts persistence FOXP3 expression Tregs subjects.Persistence was assessed by culturing sorted CD4(+)CD25(hi) natural measuring over...
Prosthetic joint infection (PJI) is a potentially limb-threatening complication of total knee arthroplasty. Phage therapy promising strategy to manage such infections including those involving antibiotic-resistant microbes, and target microbial biofilms. Experience with phage for associated retained hardware limited. A 62-year-old diabetic man history right arthroplasty 11 years prior who had suffered multiple episodes prosthetic despite numerous surgeries prolonged courses antibiotics,...
Pseudomonas strains that produce filamentous bacteriophage are associated with chronic infection and increased antibiotic resistance in patients cystic fibrosis.
Bacteriophages-viruses that infect bacteria-are abundant within our bodies, but their significance to human health is only beginning be explored. Here, we synthesize what currently known about phageome and its interactions with the immune system. We first review how phages indirectly affect immunity via bacterial expression of phage-encoded proteins. next directly influence innate clearance. Finally, discuss adaptive against implications for phage/bacterial interactions. In light these data,...
Abstract Hyaluronan is a glycosaminoglycan present in the extracellular matrix. When hyaluronan degraded during infection and injury, low m.w. forms are generated whose interactions influence inflammation angiogenesis. Intact high hyaluronan, conversely, conveys anti-inflammatory signals. We demonstrate that enhances human CD4+CD25+ regulatory T cell functional suppression of responder proliferation, whereas does not. High also up-regulates transcription factor FOXP3 on cells. These effects...
Work by our group and others has demonstrated a role for the extracellular matrix receptor CD44 its ligand hyaluronan in CD4(+)CD25(+) regulatory T cell (Treg) function. Herein, we explore mechanistic basis this observation. Using mouse FoxP3/GFP(+) Treg, find that costimulation promotes expression of FoxP3, part through production IL-2. This promotion IL-2 was resistant to cyclosporin A treatment, suggesting may promote bypassing FoxP3-mediated suppression NFAT. increased IL-10 partially...
We describe a role for ECM as biosensor inflammatory microenvironments that plays critical in peripheral immune tolerance. show hyaluronan (HA) promotes induction of Foxp3- IL-10-producing regulatory T cells (TR1) from conventional T-cell precursors both murine and human systems. This is, to our knowledge, the first description an component inducing cells. Intact HA, characteristic healing tissues, TR1 capable abrogating disease IL-10-dependent mouse colitis model whereas fragmentary typical...
Hyaluronan (HA) is an extracellular matrix glycosaminoglycan that present in pancreatic islets, but little known about its involvement the development of human type 1 diabetes (T1D). We have evaluated whether islets and lymphoid tissues T1D nondiabetic organ donors differ amount distribution HA HA-binding proteins (hyaladherins), such as inter-α-inhibitor (IαI), versican, tumor necrosis factor-stimulated gene-6 (TSG-6). was dramatically increased both within islet outside endocrine cells,...
Standard testing fails to identify a pathogen in most patients with febrile neutropenia (FN). We evaluated the ability of Karius microbial cell-free DNA sequencing test (KT) infectious etiologies FN and its impact on antimicrobial management.This prospective study (ClinicalTrials.gov; NCT02912117) enrolled analyzed 55 FN. Up 5 blood samples were collected per subject within 24 hours fever onset (T1) every 2 3 days. KT results compared culture (BC) standard microbiological (SMT)...
ABSTRACT Pseudomonas aeruginosa is an important opportunistic human pathogen that lives in biofilm-like cell aggregates at sites of chronic infection, such as those occur the lungs patients with cystic fibrosis and nonhealing ulcers. During growth a biofilm, P. dramatically increases production filamentous Pf bacteriophage (Pf phage). Previous work indicated when vivo phage was inhibited, less virulent. However, it not clear how abundant quantities similar to produced by biofilms under vitro...
Pf bacteriophage (phage) are filamentous viruses that infect Pseudomonas aeruginosa and enhance its virulence potential. virions can lyse kill P. through superinfection, which occurs when an already infected cell is by the same or similar phage.
Abstract Endothelial progenitor cells (EPCs) contribute to de novo angiogenesis, tissue regeneration, and remodeling. Interleukin 10 (IL‐10), an anti‐inflammatory cytokine that primarily signals via STAT3, has been shown drive EPC recruitment injured tissues. Our previous work demonstrated overexpression of IL‐10 in dermal wounds promotes regenerative repair STAT3‐dependent regulation fibroblast‐specific hyaluronan synthesis. However, IL‐10's role specific mode action on recruitment,...