A5102825703- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Neutropenia and Cancer Infections
- Prenatal Screening and Diagnostics
- Congenital Diaphragmatic Hernia Studies
- Bladder and Urothelial Cancer Treatments
- Infectious Aortic and Vascular Conditions
- Mollusks and Parasites Studies
- Vector-borne infectious diseases
- Sarcoma Diagnosis and Treatment
- Pneumonia and Respiratory Infections
- Parasites and Host Interactions
- Orthopedic Infections and Treatments
- Infectious Disease Case Reports and Treatments
- Extracellular vesicles in disease
- Tuberculosis Research and Epidemiology
- Diagnosis and treatment of tuberculosis
- Infectious Diseases and Mycology
- Pancreatic and Hepatic Oncology Research
- Orthopaedic implants and arthroplasty
- Parasite Biology and Host Interactions
Standard testing fails to identify a pathogen in most patients with febrile neutropenia (FN). We evaluated the ability of Karius microbial cell-free DNA sequencing test (KT) infectious etiologies FN and its impact on antimicrobial management.This prospective study (ClinicalTrials.gov; NCT02912117) enrolled analyzed 55 FN. Up 5 blood samples were collected per subject within 24 hours fever onset (T1) every 2 3 days. KT results compared culture (BC) standard microbiological (SMT)...
Noninvasive diagnostic options are limited for invasive mold infections (IMIs). We evaluated the performance of a plasma microbial cell-free DNA sequencing (mcfDNA-Seq) test diagnosing pulmonary IMI after hematopoietic cell transplant (HCT).
Abstract Background Microbial cell-free DNA (mcfDNA) sequencing of plasma can identify the presence a pathogen in host. In this study, we evaluated duration detection by mcfDNA vs conventional blood culture patients with bacteremia. Methods Blood samples from culture-confirmed bloodstream infection were collected within 24 hours index positive and 48 to 72 thereafter. was extracted plasma, next-generation applied. Reads aligned against curated database. Statistical significance defined...
Abstract Background The diagnosis of infective endocarditis (IE) can be difficult, particularly if blood cultures fail to yield a pathogen. This study evaluates the potential utility microbial cell-free DNA (mcfDNA) as tool identify etiology IE. Methods Blood samples from patients with suspected IE were serially collected. mcfDNA was extracted plasma and underwent next-generation sequencing. Reads aligned against library containing sequences belonging >1400 different pathogens....
Background: Over 1 million Americans undergo joint replacement each year, and approximately in 75 will incur a periprosthetic infection. Effective treatment necessitates pathogen identification, yet standard-of-care cultures fail to detect organisms 10% 20% of cases require invasive sampling. We hypothesized that cell-free DNA (cfDNA) fragments from microorganisms infection can be found the bloodstream utilized accurately identify pathogens via next-generation sequencing. Methods: In this...
Abstract Background Laboratory confirmation of early Lyme borreliosis (LB) is challenging. Serology insensitive during the first days to weeks infection, and blood polymerase chain reaction (PCR) offers similarly poor performance. Here, we demonstrate that detection Borrelia burgdorferi (B.b.) cell-free DNA (cfDNA) in plasma can improve diagnosis LB. Methods B.b. samples using unbiased metagenomic cfDNA sequencing performed by a commercial laboratory (Karius Inc) was compared with serology...
Intravesical instillation of bacille Calmette-Guérin (BCG), an attenuated strain Mycobacterium bovis, is adjuvant immunotherapy for bladder carcinoma. Typical complications include fever, malaise, and dysuria. However, more severe have been reported, including granulomatous pneumonitis, BCG sepsis, vascular infections. We present a case infrarenal abdominal aortic aneurysm complicated by iliopsoas abscess 2 years after treatment discuss novel diagnostic tool mycobacterial identification.
Host inflammatory responses predict worse outcome in severe pneumonia, yet little is known about what drives dysregulated inflammation. We performed metagenomic sequencing of microbial cell-free DNA (mcfDNA) 83 mechanically ventilated patients (26 culture-positive, 41 culture-negative 16 uninfected controls). Culture-positive had higher levels mcfDNA than those with pneumonia and controls (p<0.005). Plasma biomarkers (fractalkine, procalcitonin, pentraxin-3 suppression tumorigenicity-2)...
Background Chorioamnionitis has been linked to spontaneous preterm labor and complications such as neonatal sepsis. We hypothesized that microbial cell-free (cf) DNA would be detectable in maternal plasma patients with chorioamnionitis could the basis for a non-invasive method detect fetal exposure microorganisms. Objective The purpose of this study was determine whether next generation sequencing cfDNA chorioamnionitis. Study design Maternal (n = 94) umbilical cord 120) were collected...
Introduction Pulmonary invasive mold infections (IMI) remain a significant cause of morbidity and mortality after hematopoietic cell transplant (HCT). Non-invasive diagnostic options are limited, particularly for non-Aspergillus molds. Given differences in spectrum activity toxicities associated with mold-active therapy, early diagnosis targeting specific IMI is important. Objectives We evaluated the performance Karius cell-free plasma next-generation sequencing (NGS) test detecting HCT...
Abstract Background AC or Rat Lungworm meningitis usually presents as a self-limited illness with headache and sensory changes, rarely progressing to coma, death, permanent brain damage. It is diagnosed by eosinophils in the CSF. Once limited Asia tropical Pacific transmission via slugs snails documented on US mainland 2018. We describe 2 unusual, severe examples of infection infants presenting ascending paralysis initial CSF without eosinophilia suggesting Guillain–Barre syndrome (GBS)....
Abstract Background Diagnosis of invasive fungal infections (IFIs), a life-threatening complication cancer therapy or hematopoietic cell transplantation (HCT) can be challenging, and IFI has poor outcomes. Prediction early non-invasive diagnosis in high-risk hosts before onset symptoms could reduce morbidity mortality. Because plasma mcfDNA NGS detect infections, may predict bloodstream immunocompromised patients, we hypothesized that might also infection clinical presentation. Methods In...
2017 Background: Patients with malignant gliomas (MG) are exposed to intensive treatments including surgery, radiation, chemotherapy and glucocorticoids. Low serum testosterone (T) has been reported in men systemic cancers, but little is known about this phenomenon patients MG. MG often develop symptoms of fatigue muscle weakness. We hypothesized that these could be related low T conducted a retrospective study assess Methods: A total 216 male seen the Brain Cancer Program outpatient clinic...