A5046383184- Environmental remediation with nanomaterials
- Microbial bioremediation and biosurfactants
- Orthopedic Infections and Treatments
- Immunotherapy and Immune Responses
- Actinomycetales infections and treatment
- T-cell and B-cell Immunology
- Genomics and Phylogenetic Studies
- Advanced oxidation water treatment
- Orthopaedic implants and arthroplasty
- Sarcoma Diagnosis and Treatment
- Enhanced Oil Recovery Techniques
- Anaerobic Digestion and Biogas Production
- Mycobacterium research and diagnosis
- Infective Endocarditis Diagnosis and Management
- Bacterial Identification and Susceptibility Testing
- vaccines and immunoinformatics approaches
- Microbial Community Ecology and Physiology
- Antibiotic Resistance in Bacteria
- Microbial Fuel Cells and Bioremediation
Complete Genomics (United States)
2023
ABSTRACT Reductive dehalogenation of vinyl chloride (VC) to ethene is the key step in complete anaerobic degradation chlorinated ethenes. VC-reductive dehalogenase was partially purified from a highly enriched culture VC-respiring Dehalococcoides sp. strain VS. The enzyme reduced VC and all dichloroethene (DCE) isomers, but not tetrachloroethene (PCE) or trichloroethene (TCE), at high rates. By using reversed genetics, corresponding gene ( vcrA ) isolated characterized. Based on predicted...
ABSTRACT Shewanella oneidensis MR-1 is a facultative sediment microorganism which uses diverse compounds, such as oxygen and fumarate, well insoluble Fe(III) Mn(IV) electron acceptors. The donor spectrum more limited includes metabolic end products of primary fermenting bacteria, lactate, formate, hydrogen. While the utilization hydrogen an has been described previously, we report here formation from pyruvate under anaerobic, stationary-phase conditions in absence external acceptor. Genes...
Background: Over 1 million Americans undergo joint replacement each year, and approximately in 75 will incur a periprosthetic infection. Effective treatment necessitates pathogen identification, yet standard-of-care cultures fail to detect organisms 10% 20% of cases require invasive sampling. We hypothesized that cell-free DNA (cfDNA) fragments from microorganisms infection can be found the bloodstream utilized accurately identify pathogens via next-generation sequencing. Methods: In this...
Abstract Background The variable clinical presentation of IE requires a diagnostic tool that accurately detects wide range organisms, including in culture-negative (CN) scenarios. A sensitive molecular assay quantitates pathogen DNA could be useful to diagnose and evaluate response antimicrobial therapy. Methods We prospectively enrolled 30 hospitalized adult patients evaluated for acute classified using the Duke Criteria. Residual plasma samples within 24 hours and/or fresh whole blood...
Abstract The major histocompatibility complex (MHC) is a region of the human genome that key to immune system function but sometimes refractory genomic analyses due extreme polymorphism and structural variation. We performed targeted long-read sequencing de novo assembly MHC create 246 highly accurate, fully contiguous, phased full-length sequences, mostly from data provided by Human Pangenome Reference Consortium (HPRC). identified alleles at high resolution across 39 loci including class I...