A5101780940- Mycobacterium research and diagnosis
- Vector-borne infectious diseases
- Antifungal resistance and susceptibility
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Vectors
- Antibiotic Resistance in Bacteria
- Fungal Infections and Studies
- Infectious Diseases and Mycology
- Immunodeficiency and Autoimmune Disorders
- Vector-Borne Animal Diseases
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Orthopaedic implants and arthroplasty
- Gut microbiota and health
- Autoimmune Neurological Disorders and Treatments
- Neurological Disease Mechanisms and Treatments
- Hematopoietic Stem Cell Transplantation
- Lymphoma Diagnosis and Treatment
- Dementia and Cognitive Impairment Research
- Tissue Engineering and Regenerative Medicine
- Transplantation: Methods and Outcomes
- Pneumocystis jirovecii pneumonia detection and treatment
- Zoonotic diseases and public health
- Electrospun Nanofibers in Biomedical Applications
Stanford University
2017
Stanford Medicine
2017
Hong Kong Sanatorium and Hospital
2007-2010
Experimental studies have demonstrated that bone marrow (BM) cells can induce angiogenesis in ischaemic myocardium. Recently, several non-randomized pilot also suggested direct BM implantation appears to be feasible and safe patients with severe coronary artery diseases (CAD). We performed a randomized, blinded, placebo-controlled trial 28 CAD patients. After harvesting, we assigned receive low dose (1 × 106 cells/0.1 mL, n = 9), high (2 10) autologous or control (0.1 mL plasma/injection, 9)...
Noninvasive diagnostic options are limited for invasive mold infections (IMIs). We evaluated the performance of a plasma microbial cell-free DNA sequencing (mcfDNA-Seq) test diagnosing pulmonary IMI after hematopoietic cell transplant (HCT).
Abstract Background Microbial cell-free DNA (mcfDNA) sequencing of plasma can identify the presence a pathogen in host. In this study, we evaluated duration detection by mcfDNA vs conventional blood culture patients with bacteremia. Methods Blood samples from culture-confirmed bloodstream infection were collected within 24 hours index positive and 48 to 72 thereafter. was extracted plasma, next-generation applied. Reads aligned against curated database. Statistical significance defined...
Abstract Background The diagnosis of infective endocarditis (IE) can be difficult, particularly if blood cultures fail to yield a pathogen. This study evaluates the potential utility microbial cell-free DNA (mcfDNA) as tool identify etiology IE. Methods Blood samples from patients with suspected IE were serially collected. mcfDNA was extracted plasma and underwent next-generation sequencing. Reads aligned against library containing sequences belonging >1400 different pathogens....
<h3>Objective</h3> Although age-related confluent white-matter lesion (WML) is an important substrate for cognitive impairment, the mechanisms whereby WML induces impairment are uncertain. The authors investigated predictors in patients with WML. <h3>Methods</h3> Among 100 ischaemic stroke on MRI, assessed executive function and global cognition by Mattis Dementia Rating Scale—Initiation/Perseveration Subscale (MDRS I/P) Mini-Mental State Examination (MMSE), respectively. All volumetric...
Background: Over 1 million Americans undergo joint replacement each year, and approximately in 75 will incur a periprosthetic infection. Effective treatment necessitates pathogen identification, yet standard-of-care cultures fail to detect organisms 10% 20% of cases require invasive sampling. We hypothesized that cell-free DNA (cfDNA) fragments from microorganisms infection can be found the bloodstream utilized accurately identify pathogens via next-generation sequencing. Methods: In this...
Abstract Background Laboratory confirmation of early Lyme borreliosis (LB) is challenging. Serology insensitive during the first days to weeks infection, and blood polymerase chain reaction (PCR) offers similarly poor performance. Here, we demonstrate that detection Borrelia burgdorferi (B.b.) cell-free DNA (cfDNA) in plasma can improve diagnosis LB. Methods B.b. samples using unbiased metagenomic cfDNA sequencing performed by a commercial laboratory (Karius Inc) was compared with serology...
Host inflammatory responses predict worse outcome in severe pneumonia, yet little is known about what drives dysregulated inflammation. We performed metagenomic sequencing of microbial cell-free DNA (mcfDNA) 83 mechanically ventilated patients (26 culture-positive, 41 culture-negative 16 uninfected controls). Culture-positive had higher levels mcfDNA than those with pneumonia and controls (p<0.005). Plasma biomarkers (fractalkine, procalcitonin, pentraxin-3 suppression tumorigenicity-2)...
Abstract Background Sepsis is a leading cause of death and can be caused by wide range potential pathogens. In up to 40% cases, causative pathogen never identified. There need for improved diagnostic tests that accurately identify the breadth pathogens inform effective antimicrobial therapy. Methods We enrolled prospective cohort patients presenting hospital with signs symptoms sepsis. Plasma samples were collected NGS testing at time initial blood culture. Extracted plasma cell-free DNA was...
Introduction Pulmonary invasive mold infections (IMI) remain a significant cause of morbidity and mortality after hematopoietic cell transplant (HCT). Non-invasive diagnostic options are limited, particularly for non-Aspergillus molds. Given differences in spectrum activity toxicities associated with mold-active therapy, early diagnosis targeting specific IMI is important. Objectives We evaluated the performance Karius cell-free plasma next-generation sequencing (NGS) test detecting HCT...
We conducted an exploratory study of plasma microbial cell-free DNA sequencing for the diagnosis Lyme disease among pediatric patients. Low levels Borrelia burgdorferi (<3–5 molecules per microliter) were observed in 6/9 serologically confirmed participants, including 4/5 with arthritis and 2/3 multiple erythema migrans.
Abstract Background Diagnosing Lyme disease often involves laboratory evaluation, yet available tests have limitations. Serology remains negative for weeks after infection occurs, and may then remain positive years. Borrelia burgdorferi blood PCR testing has low sensitivity, rendering it unhelpful. We sought to determine whether an emerging technology, next-generation sequencing (NGS) of microbial cell-free DNA (mcfDNA), can detect B. in the plasma pediatric patients with erythema migrans...
Purpose:The United States Advisory Committee on Immunization Practices recommends that PCV13 be given in addition to 23-valent pneumococcal polysaccharide vaccine immunocompromised adults, such as chronic renal failure and SOT. However, data regarding this is sparse. The present study aimed assess the immunogenicity safety of SOT candidates recipients. Methods: Consecutive patients receiving pre-SOT evaluation receipts with stable clinical conditions 6 months post transplant were enrolled....
Abstract Background Pulmonary invasive mold infections (IMI) cause significant morbidity and mortality after hematopoietic cell transplant (HCT). Noninvasive diagnostic options are limited, particularly for non-Aspergillus (non-Asp) molds. Given differences in activity toxicities of antifungals, early diagnosis targeting specific IMI is important. We evaluated the performance Karius Test, a plasma microbial cell-free DNA next-generation sequencing (NGS) test, detecting HCT recipients....
Background: Erythema migrans (EM), the skin rash of early Lyme borreliosis (LB), is an insensitive and non-specific physical finding. Confirmation suspected cases challenging, as serology blood PCR are in LB. Here, we demonstrate that detection Borrelia burgdorferi (B.b.) cell-free DNA (cfDNA) plasma can improve diagnosis LB.Methods: We compared B.b.cfDNA samples with 40 patients physician-diagnosed EM, 28 whom were confirmed to have LB by biopsy culture (N=18), seroconversion (N=2) or both...