A5034290455- RNA regulation and disease
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Wheat and Barley Genetics and Pathology
- Cancer-related molecular mechanisms research
Massachusetts Institute of Technology
2021-2024
Whitehead Institute for Biomedical Research
2024
Howard Hughes Medical Institute
2015-2021
Yale University
2015
Herpesvirus saimiri (HVS) is an oncogenic γ-herpesvirus that produces microRNAs (miRNAs) by cotranscription of precursor miRNA (pre-miRNA) hairpins immediately downstream from viral small nuclear RNAs (snRNA). The host cell Integrator complex, which recognizes the snRNA 3′ end processing signal (3′ box), generates 5′ ends HVS pre-miRNA hairpins. Here, we identify a novel box-like sequence (miRNA box) pre-miRNAs essential for biogenesis. In vivo knockdown and rescue experiments confirmed also...
Aneuploid yeast cells are in a chronic state of proteotoxicity, yet do not constitutively induce the cytosolic unfolded protein response, or heat shock response (HSR) by factor 1 (Hsf1). Here, we demonstrate that an active environmental stress (ESR), hallmark aneuploidy across different models, suppresses Hsf1 induction models single-chromosome gain. Furthermore, engineered activation ESR absence was sufficient to suppress euploid subsequent while increasing thermotolerance and blocking...
Abstract In mammalian cells, the integrated stress response (ISR) triggers ATF4 translation under general conditions of cell stress. Mammalian target rapamycin (mTOR) signaling also pro-growth conditions. While transcriptomic changes these two contradictory pathways are have been studied, a full understanding all capable increasing and how regulated interact with each other remains unknown. genome-wide CRISPRi screen, we found that loss CARS is sufficient to activate independent canonical...