A5035277433- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Microbial Fuel Cells and Bioremediation
- Nuclear Structure and Function
- Metal Extraction and Bioleaching
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- Cancer therapeutics and mechanisms
- Muscle Physiology and Disorders
- Corrosion Behavior and Inhibition
- Endoplasmic Reticulum Stress and Disease
- Fungal and yeast genetics research
- Wheat and Barley Genetics and Pathology
- Neonatal and fetal brain pathology
- Prenatal Screening and Diagnostics
- Synthesis and bioactivity of alkaloids
- Chromosomal and Genetic Variations
- Chromium effects and bioremediation
- Gene Regulatory Network Analysis
- Leptospirosis research and findings
- SARS-CoV-2 detection and testing
- RNA regulation and disease
- Hemoglobinopathies and Related Disorders
- Cerebrospinal fluid and hydrocephalus
- Vitamin C and Antioxidants Research
Memorial Sloan Kettering Cancer Center
2024
Texas A&M University
2024
Pfizer (United States)
2021-2022
Massachusetts Institute of Technology
2017-2021
Howard Hughes Medical Institute
2000-2021
Providence College
2012-2014
University of Virginia Health System
2012
Brigham and Women's Hospital
2010
Harvard University
2010
Evotec (United Kingdom)
2008-2009
AU-rich elements (AREs) present in the 3′ untranslated regions of many protooncogene, cytokine, and lymphokine messages target them for rapid degradation. HuR, a ubiquitously expressed member ELAV (embryonic lethal abnormal vision) family RNA binding proteins, selectively binds AREs stabilizes ARE-containing mRNAs transiently transfected cells. Here, we identify four mammalian proteins that bind HuR known to be essential its ability shuttle between nucleus cytoplasm stabilize mRNA: SETα,...
AU-rich elements (AREs) located in the 3′ untranslated region target mRNAs encoding many protooncoproteins, cytokines, and lymphokines for rapid degradation. HuR, a ubiquitously expressed member of embryonic lethal abnormal vision (ELAV) family RNA-binding proteins, binds ARE sequences selectively stabilizes ARE-containing reporter when overexpressed transiently transfected cells. HuR appears predominantly nucleoplasmic but has been shown to shuttle between nucleus cytoplasm via novel...
Aneuploidy, a condition characterized by chromosome gains and losses, causes reduced fitness numerous cellular stresses, including increased protein aggregation. Here, we identify complex stoichiometry imbalances as major cause of aggregation in aneuploid cells. Subunits complexes encoded on excess chromosomes aggregate cells, which is suppressed when expression other subunits coordinately altered. We further show that are either degraded or nearly effective degradation at lowering levels...
Facioscapulohumeral muscular dystrophy (FSHD) is caused by misexpression of the DUX4 transcription factor in skeletal muscle that results transcriptional alterations, abnormal phenotypes and cell death. To gain insight into kinetics DUX4-induced stresses, we activated expression myoblasts performed longitudinal RNA sequencing paired with proteomics phosphoproteomics. This analysis revealed changes cellular physiology upon activation, including DNA damage altered mRNA splicing....
Abstract Background Hfq is an RNA chaperone protein that has been broadly implicated in sRNA function bacteria. Here we describe the construction and characterization of a null allele gene encodes Shewanella oneidensis strain MR-1, dissimilatory metal reducing bacterium. Results Loss hfq S. results variety mutant phenotypes, all which are fully complemented by addition plasmid-borne copy wild type gene. Aerobic cultures hfq∆ grow more slowly through exponential phase than cultures, reach...
Aneuploid yeast cells are in a chronic state of proteotoxicity, yet do not constitutively induce the cytosolic unfolded protein response, or heat shock response (HSR) by factor 1 (Hsf1). Here, we demonstrate that an active environmental stress (ESR), hallmark aneuploidy across different models, suppresses Hsf1 induction models single-chromosome gain. Furthermore, engineered activation ESR absence was sufficient to suppress euploid subsequent while increasing thermotolerance and blocking...
Kirsten rat sarcoma (KRAS) is a frequently mutated oncogene responsible for several oncogenic KRAS variants and driving tumor proliferation. Some nonsmall cell lung cancer (NSCLC) tumors exhibit G12C mutations, which can be targeted inhibition using covalent more recently noncovalent inhibitors. Sotorasib was the first FDA-approved inhibitor that has shown efficacy in patients, but with mixed responses. The lack of attributed to heterogeneity (lack mutations) and/or inefficient delivery....
The RNA chaperone Hfq fulfills important roles in small regulatory (sRNA) function many bacteria. Loss of the dissimilatory metal reducing bacterium Shewanella oneidensis strain MR-1 results slow exponential phase growth and a reduced terminal cell density at stationary phase. We have found that defect hfq mutant LB is result heme levels. Both levels can be completely restored by supplementing medium with 5-aminolevulinic acid (5-ALA), first committed intermediate synthesized during...
Shewanella oneidensis is a dissimilatory metal reducing bacterium that can utilize range of terminal electron acceptors when grown under anaerobic conditions. Our primary goal to identify and characterize genes encode small regulatory non‐coding RNAs (sRNAs) in S. . sRNA are regulated by changes environmental conditions themselves regulate other inexact base pairing their mRNA targets. We particularly interested sRNAs may the process reduction. The highly conserved RNA chaperone protein Hfq...