A5094222268- Cancer, Lipids, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- ATP Synthase and ATPases Research
- RNA modifications and cancer
The University of Texas Health Science Center at San Antonio
2024
<p>Figure S1. Levels of PELP1 expression in 6 HCC cell lines.</p>
<p>Figure S2. SMIP34 treatment downregulates liver specific genes, MYC and E2F pathway targeted genes in HCC cells.</p>
Abstract Hepatocellular carcinoma (HCC) is one of the leading causes cancer-related deaths in United States, with a median survival period approximately 10 months. There an urgent need for development effective targeted therapies treatment HCC. Proline-, glutamic acid- and leucine-rich protein 1 (PELP1) signaling implicated progression many cancers, although its specific contribution to HCC not yet well understood. Analysis TCGA gene expression data sets immunohistochemistry analysis tissue...
Abstract Background: Hepatocellular carcinoma (HCC) is the fastest-rising cause of cancer-related mortality in United States. Furthermore, advanced HCC has a very poor prognosis, with median survival time only about ten months. Effective new targeted therapies are urgently needed for treatment HCC. Proline, glutamic acid-, and leucine-rich protein 1 (PELP1) protooncogene. PELP1 signaling been implicated development several cancers, however, its role progression remains unclear. The goal this...
<div>Abstract<p>Hepatocellular carcinoma (HCC) is one of the leading causes cancer-related deaths in United States, with a median survival period approximately 10 months. There an urgent need for development effective targeted therapies treatment HCC. Proline-, glutamic acid–, and leucine-rich protein 1 (PELP1) signaling implicated progression many cancers, although its specific contribution to HCC not yet well understood. Analysis The Cancer Genome Atlas gene expression data...
<p>Analysis of global transcriptional changes in <i>PELP1</i>-KD HCC cells. Volcano plot differentially expressed genes with PELP1-KD Hep3B cells is displayed (<i>n</i> = 3; <b>A</b>). PELP1-downregulated pathways were identified using gene signature (<b>B</b>). Gene set enrichment analysis plots show negatively enriched by (<b>C</b>). Heatmap images the specific affected (<b>D</b>). RT-qPCR was used to validate...
<p>PELP1 expression is upregulated in HCC, and high PELP1 associated with poor survival of patients HCC. Data obtained from TNMplot shows increased HCC (<b>A</b>). The results TCGA-UALCAN database show that progression (<b>B</b>). Clinical Proteomic Tumor Analysis Consortium tumor tissues compared normal (<b>C</b>). Association protein overall was Human Protein Atlas (<b>D</b>). TMA used to investigate the 86 six liver specimens by IHC...
<p><i>PELP1</i>-KD or SMIP34 treatment suppresses HCC xenograft tumor growth <i>in vivo</i>. Hep3B–control and Hep3B–<i>PELP1</i>-KD model cells were injected subcutaneously into female (<b>A</b>, <b>D</b>, <b>G</b>) male SCID mice (<b>B</b>, <b>E</b>, <b>H</b>). Tumor volumes assessed at 3 to 5 days of intervals. volume (<b>A</b> <b>B</b>), weights...
<p>Figure S1. Levels of PELP1 expression in 6 HCC cell lines.</p>
<p>Figure S2. SMIP34 treatment downregulates liver specific genes, MYC and E2F pathway targeted genes in HCC cells.</p>
<p><i>PELP1</i>-KD/SMIP34 treatment decreased cell viability, clonogenicity, and invasiveness of HCC cells. PELP1-KD in Huh7 Hep3B lines were confirmed by Western blot (<b>A</b>). Cell viability clonogenic assays performed to assess the impact PELP1 KD on growth cells (<b>B</b> <b>C</b>). The effect <i>PELP1</i>-KD invasion was using Matrigel chamber (<b>D</b>). SMIP34 six measured MTT assay (<b>E</b>)....
<p>SMIP34 treatment blocked PELP1-mediated extranuclear signaling and decreased global protein synthesis. Huh7, SNU398, Hep3B, SNU423 cells were treated with vehicle (DMSO 0.01%) or SMIP34 (12.5 μmol/L) to examine PELP1 degradation the stability of Rix1 complex by Western blotting (<b>A</b> <b>C</b>). SNU449 μmol/L), status known downstream targets analyzed (<b>B</b>). Hep3B Huh7 E2F1 c-Myc expressions was (<b>D</b>). (5, 10, 15 μm)...