A5009870201- Tryptophan and brain disorders
- Pain Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- T-cell and B-cell Immunology
- Stress Responses and Cortisol
- Cancer Treatment and Pharmacology
- Connexins and lens biology
- Adolescent and Pediatric Healthcare
- RNA regulation and disease
- Psychosomatic Disorders and Their Treatments
- Child Development and Education
- Immunotherapy and Immune Responses
- Pregnancy-related medical research
- Bacterial Infections and Vaccines
- Maternal Mental Health During Pregnancy and Postpartum
- Ion channel regulation and function
- Autism Spectrum Disorder Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Birth, Development, and Health
- Human Health and Disease
- Glioma Diagnosis and Treatment
- Infectious Encephalopathies and Encephalitis
- Multiple Sclerosis Research Studies
- melanin and skin pigmentation
- Cannabis and Cannabinoid Research
Sydney Children’s Hospitals Network
2025
Children's Hospital at Westmead
2023-2025
The University of Sydney
2023
UNSW Sydney
2017-2023
Sensory problems such as neuropathic pain are common and debilitating symptoms in multiple sclerosis (MS), an autoimmune inflammatory disorder of the CNS. Regulatory T (Treg) cells critical for maintaining immune homeostasis, but their role MS-associated remains unknown. Here, we demonstrate that Treg cell ablation is sufficient to trigger experimental encephalomyelitis (EAE) facial allodynia immunized female mice. In EAE-induced mice, adoptive transfer spinal delivery cytokine...
OPINION article Front. Neurosci., 15 October 2019 | https://doi.org/10.3389/fnins.2019.01107
Immune cells play a critical role in promoting neuroinflammation and the development of neuropathic pain. However, some subsets immune are essential for pain resolution. Among them regulatory T (Tregs), specialised subpopulation that limit excessive responses preserve homeostasis. In this study, we utilised intrathecal adoptive transfer activated Tregs male female mice after peripheral nerve injury to investigate Treg migration whether Treg-mediated suppression behaviours is associated with...
DNA variations in the NF-kappa-B essential modulator (NEMO) gene are linked to incontinentia pigmenti (IP) and also immunodeficiency autoinflammatory conditions. Some patients with IP present neonatal vasculitis-like brain changes, although pathogenesis is unclear. We investigated cell-specific expression a neonate IP, who had encephalopathy, seizures, responded steroid treatment. Single-cell RNA (ribonucleic acid) sequencing (scRNAseq), using HIVE single-cell system, was performed on before...
Neurodevelopmental including autistic regression in children is often associated with inconclusive diagnostic investigations, leaving the underlying pathophysiology largely unexplored and poorly understood. To address this, we conducted peripheral blood multi-omics profiling 15 neurodevelopmental (median age 9 (4-14) years, 60% males), healthy controls 12 (7-16) males). Our comprehensive analysis included bulk RNA sequencing (8 vs. 8 controls), single-cell (two cohorts of 4 proteomics (4...
Environmental exposures across critical developmental windows can significantly influence brain development and contribute to the pathogenesis of neurodevelopmental disorders (NDDs). Emerging clinical evidence suggests that cumulative environmental factors during early result in more pronounced disease phenotypes offspring. Here, we developed a triple-hit model using C57Bl/6JAusb mice examine effects antenatal social stress, chronic high-fat diet consumption, postnatal poly(I:C) exposure on...
Paediatric acute-onset neuropsychiatric syndrome (PANS) is characterised by infection provoked abrupt and dramatic onset of obsessive compulsive disorder (OCD) or eating restriction, along with neurodevelopmental regression. Although the aetiology PANS remains uncertain, due to a prevailing neuroimmune hypothesis some children receive immune modulator intravenous immunoglobulin (IVIg). Using single-cell RNA sequencing peripheral cells, we examined gene expression in five (mean age 10.2...
Advances in Complementary & Alternative medicine Safety and Efficacy of Orally Administered Full-spectrum Medicinal Cannabis Plant Extract 0.08% THC (NTI164) Children with Autism Spectrum Disorder Dima El Sukkari1, Kanan Sharma1, Bobbi Fleiss2, Dion L Braganza1, Brooke A Keating3*, Yelda Ogru3, Esra Isikgel3, Alison Crichton4,5 Michael C Fahey1 1Monash Children's Clinical Trial Centre, Monash Hospital, Clayton, Victoria, Australia 2Health Biomedical Sciences, RMIT University, STEM College,...
Paediatric Acute-Onset Neuropsychiatric Syndrome (PANS) is characterised by abrupt onset obsessive compulsive disorder and regression in neurodevelopmental skills, triggered infection or stress. Whether PANS a distinct entity part of spectrum uncertain, its pathophysiology remains unclear. We show that children with (n=32) other non-PANS (n=68) disorders (total n=100) have higher reported early childhood infections loss previously acquired developmental skills compared to neurotypical...
Haematological toxicities occur in patients receiving oxaliplatin. Mild anaemia (grade 1-2) is a common side effect and approximately 90% of recipients develop measurable spleen enlargement. Although generally asymptomatic, oxaliplatin-induced splenomegaly independently associated with complications following liver resection for colorectal metastasis separately poorer patient outcomes. Here, we investigated haematological mice treated escalating dosages comparable to those prescribed cancer...
A modern approach for cancer treatment is the use of immunotherapy, and particularly immune checkpoint inhibitors, such as anti-programmed cell death protein 1 (PD-1), alone in combination with chemotherapy. The PD-1 pathway plays a crucial role inhibiting responses recently has been shown to modulate neuronal activity. However, impact blockade on development chemotherapy-induced peripheral neuropathy currently unknown. In this study, we show that C57BL/6 mice treated chemotherapeutic drug...
Abstract Introduction/Aims Clinically, the chemotherapeutic agent oxaliplatin can cause peripheral neuropathy, impaired balance, and muscle wastage. Using a preclinical model, we investigated whether exercise intervention could improve these adverse conditions. Methods Mice were chronically treated with alone or in conjunction exercise. Behavioral studies, including mechanical allodynia, rotarod, open‐field, grip‐strength tests, performed. After euthanasia, multiple organs four different...
Abstract Peripheral nerve injuries caused by focal constriction are characterised local ischaemia, axonal degeneration, demyelination, and neuroinflammation. The aim of this study was to understand temporal changes in the excitability properties injured motor axons a mouse model injury (NCI). following unilateral sciatic NCI studied male C57BL/6J mice distal site at acute (6 hours‐1 week) chronic (up 20 weeks) phases injury, using threshold tracking. Multiple measures excitability, including...