A5002998279- Monoclonal and Polyclonal Antibodies Research
- Rheumatoid Arthritis Research and Therapies
- Complement system in diseases
- Systemic Lupus Erythematosus Research
- Renal Diseases and Glomerulopathies
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
- HER2/EGFR in Cancer Research
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Chronic Lymphocytic Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Radiopharmaceutical Chemistry and Applications
- Diabetes and associated disorders
- Toxin Mechanisms and Immunotoxins
- Thyroid Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Salivary Gland Disorders and Functions
- Spondyloarthritis Studies and Treatments
- Phagocytosis and Immune Regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Hemophilia Treatment and Research
- Protein Interaction Studies and Fluorescence Analysis
- Extracellular vesicles in disease
Bar-Ilan University
2023
University of Manchester
2012
Wellcome Centre for Cell-Matrix Research
2012
University Hospital of Wales
1985-2011
Cardiff University
2006-2010
University of Wales
1985-2000
Institute of Science and Technology
1989
Hammersmith Hospital
1978-1987
Hôpital Necker-Enfants Malades
1977
London Postgraduate Medical and Dental Education
1976-1977
Investigation of the serum complement system in 25 patients with various forms lipodystrophy showed no abnormality three total lipodystrophy; a single patient limb had evidence activation classical pathway. However, 21 partial lipodystrophy, 17 low C3, normal C4 and C2, concentrations, accompanied 14 by C3 splitting factor indistinguishable from nephritic factor, suggesting alternative These abnormalities occurred 10 without clinically overt renal disease. Seven nephritis; biopsies obtained...
Objectives . Despite recent major advances in the understanding of pathogenesis rheumatoid arthritis, with tumour necrosis factor-alpha (TNFα) established as a therapeutic target, comparatively little is known about mediators involved destructive and inflammatory pathways osteoarthritis (OA). Recently, it has become appreciated that an synovitis contributes not only to signs symptoms osteoarthritis, but also disease progression. Here, we use high-efficiency adenoviral gene transfer...
The metabolism of the fifth component complement (C5), and its relatonship to third (C3), has been studied in normal subjects patients by simultaneous administration radioiodine labeled C5 C3. In seven fractional catabolic rate ranged from 1.5 2.1% plasma pool/h extravascular/intravascular distribution ratio 0.22 0.78, these values being similar those obtained for C3, synthesis 71 134 mug/kg per h, with activation increase was nearly always less than that data also showed there increased...
Glioblastoma (GBM) is the most common and aggressive primary brain tumor. GBM contains a small subpopulation of glioma stem cells (GSCs) that are implicated in treatment resistance, tumor infiltration, recurrence, thereby considered important therapeutic targets. Recent clinical studies have suggested choice general anesthetic (GA), particularly propofol, during resection, affects subsequent response to treatments patient prognosis. In this study, we investigated molecular mechanisms...
Technetium-99m labelled, negatively charged liposomes accumulate in the inflamed tissue of rats with adjuvant induced arthritis. Up to 10 times more liposome accumulation was seen paws than control rats, and this represented 5.3% injected dose. The directly related size, maximal occurring less 100 nm diameter.
Abstract. We have investigated the prevalence of proteinuria in patients with Graves' disease and chronic autoimmune thyroiditis attending a routine thyroid clinic. Using urine protein creatinine index, was found 29.8% 9.5% same clinic but without these conditions. When were treated 131 I, measured by 24 h collections developed 9 14 pre-existing appeared to diminish 4 whom had been present before treatment. The fluctuation independent thyroglobulin microsomal antibody levels. unable confirm...
High performance liquid chromatography has shown that after intravenous injection cholesterol-poor liposomes (100 nm) are unstable and their phospholipid is redistributed. Under identical conditions cholesterol-rich remain structurally intact within the circulation. When injected intravenously accumulate inflamed paws of rats with adjuvant induced arthritis to same extent as liposomes. Uptake in tissue three liposome preparations was always significantly greater than uptake noted normal...
SUMMARY Clodronate, encapsulated within small unilamellar vesicles (SUVc) will deplete hepatic macrophages after intravenous injection. Functional studies, using probes to evaluate Fc and C3b uptake, showed a close correlation between the inhibition of receptor-mediated uptake depletion macrophages. Twenty milligrams clodronate SUVc produced · 90% clearance Fc- C3b-coated erythrocytes comparable reduction macrophage numbers. Inhibition these erythroctyes was closely related in Repopulation...
Abstract Four human monoclonal rheumatoid factors (MRF) were used to raise a panel of mouse antibodies (mAb) which selected in solid‐phase radioimmunoassay for binding MRF but not normal IgM. Three mAb, each raised against different MRF, bound the majority and also most polyclonal RF. other mAb selectively they no rarely any Competition studies using cold radiolabeled further indicated that these recognize distinct epitopes on MRF. RF activity was inhibited by 3 4 single 2 multiple It thus...
AbstractUsing liposomes radiolabelled by the 99mTechnetium-stannous chloride technique we have investigated effect of surface charge on stability isotope in vitro and vivo. Dialysis 99mTc-labelled positive, negative neutral liposomes, which had been incubated either saline or normal rat serum showed no significant loss from liposome with only 2 per cent dialysed. A comparison gel chromatography dialysis confirmed that most remained attached to surface, but it did reveal greater isotope,...
To investigate the effects of aminobisphosphonate, ibandronate, on course joint inflammation in rat antigen-induced arthritis (AIA) and release pro-inflammatory cytokines partially purified human peripheral blood mononuclear cells (PBMC).Rats with AIA received a single intra-articular injection ibandronate (1 mg) 7 days post-arthritis induction knee swelling was measured for thereafter. The (300 microg/ml) PBMC cytokine production activation marker expression were determined using polymerase...
SUMMARY In this study we evaluated the comparative efficacy of free and liposomally conjugated methotrexate on both disease indtiction suppression acute inflammation in rat adjuvant-induced arthritis. Rats were given either empty liposomes (E-LIPO), (MTX) or (MTX-LIPO) at a dose 100/μg/day for 7 consecutive days by intravenous route. When MTX treatment was initiated day arthritis induction drug suppressed bul did not abolish development joint inflammation. Free had no significant...
Two techniques have been studied for their suitability the analysis of stability liposomes: (1) High Performance Gel Permeation Liquid Chromatography (HPGPLC), a TSK G5000PW Ultrogel column; (2) (GPC), Sepharose 4B column. The dual radio-labelled, cholesterol-poor and cholesterol-rich, negatively charged liposomes in vitro (in saline serum), vivo, investigated using these two techniques. HPGPLC column proved to be superior technique liposome with advantages rapid run times, increased sample...
Liposomes with conventional and long‐circulation times were employed as carriers for the methotrexate derivative MTX‐γ‐DMPE (MTX‐EPC MTX‐PEG respectively), their mechanism of action was investigated in vitro vivo therapeutic efficacy assessed using rat collagen‐induced arthritis (CIA) model. At non‐toxic dose, both MTX‐EPC inhibited lipopolysaccharide (LPS) induced release IL‐1β from activated peritoneal macrophages (rPMΦ) a dose time dependent manner. Free (MTX) not active this respect....
Journal Article THE EFFECT OF LIPOSOMALLY CONJUGATED METHOTREXATE UPON MEDIATOR RELEASE FROM HUMAN PERIPHERAL BLOOD MONOCYTES Get access A. S. WILLIAMS, WILLIAMS Rheumatology Research Laboratory, University of Wales College MedicineHeath Park, Cardiff CF4 4XN Correspondence to: Williams. Search for other works by this author on: Oxford Academic PubMed Google Scholar Y. L. PUNN, PUNN N. AMOS, AMOS M. COOPER, COOPER B. D. Rheumatology, Volume 34, Issue 3, March 1995, Pages 241–245,...
A monoclonal rheumatoid factor binding assay has been employed to detect IgG type immune complexes in sera from 21 patients with minor recurrent aphthous ulceration and 31 control subjects. In only one of the ulcer group was there a marginal elevation above normal range, no statistically significant difference between patient groups. Assays intrinsic serum IgG, IgM IgA levels also showed differences groups, all mean values falling within ranges.