A5052457438- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Molecular Biology Techniques and Applications
- Dementia and Cognitive Impairment Research
- Memory and Neural Mechanisms
- S100 Proteins and Annexins
- Amyotrophic Lateral Sclerosis Research
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Neurological diseases and metabolism
- Parkinson's Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Advanced Fluorescence Microscopy Techniques
- Traumatic Brain Injury Research
- Regulation of Appetite and Obesity
- Neurogenesis and neuroplasticity mechanisms
- Tryptophan and brain disorders
- Lysosomal Storage Disorders Research
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Ischemic Stroke Management
- Carbohydrate Chemistry and Synthesis
- Glycogen Storage Diseases and Myoclonus
- Neurogenetic and Muscular Disorders Research
Universität Ulm
2013-2024
Helmholtz-Institute Ulm
2022-2023
Objective To see whether the distribution patterns of phosphorylated 43kDa TAR DNA‐binding protein (pTDP‐43) intraneuronal inclusions in amyotrophic lateral sclerosis (ALS) permit recognition neuropathological stages. Methods pTDP‐43 immunohistochemistry was performed on 70μm sections from ALS autopsy cases (N = 76) classified by clinical phenotype and genetic background. Results with lowest burden pathology were characterized lesions agranular motor cortex, brainstem nuclei cranial nerves...
Abstract Background Post mortem human brain tissue is an essential resource to study cell types, connectivity as well subcellular structures down the molecular setup of central nervous system especially with respect plethora diseases. A key method immunostaining fluorescent dyes, which allows high-resolution imaging in three dimensions multiple simultaneously. Although there are large collections formalin-fixed brains, research often limited because several conditions arise that complicate...
Amyotrophic Lateral Sclerosis (ALS) is mainly characterized by the degeneration of corticospinal neurons and spinal α-motoneurons; vulnerable cells display prominent pTDP-43 inclusions. Evidence gathered from genetics, murine models, iPSC-derived point to early involvement synapses in disease course their crucial role pathogenic cascade. However, pathology studies, with specimens large post-mortem cohorts, mapping pattern synaptic disturbances over clinical neuropathological hallmarks...
Although Gaucher disease can be accompanied by Lewy pathology (LP) and extrapyramidal symptoms, it is unknown if LP exists in Fabry (FD), another progressive multisystem lysosomal storage disorder. We aimed to elucidate the distribution patterns of FD-related inclusions brain a 58-year-old cognitively unimpaired male FD patient suffering from predominant hypokinesia. Immunohistochemistry (CD77, α-synuclein, collagen IV) neuropathological staging were performed on 100-µm sections. Tissue...
Abstract We quantified and determined for the first time distribution pattern of neuropeptide NPFF in human cerebral cortex subjacent white matter. To do so, we studied n = 9 cases without neurological disorders 22 with neurodegenerative diseases, including sporadic amyotrophic lateral sclerosis (ALS, 8), Alzheimer’s disease (AD, Pick’s (PiD, 3), schizophrenia ( 3). NPFF-immunopositive cells were located chiefly, but not exclusively, superficial matter constituted there a subpopulation...
Cortical microinfarcts are the most widespread form of brain infarction but frequently remain undetected by standard neuroimaging protocols. Moreover, only partially detectable in hematoxylin-eosin-stained (H and E) 4-10 µm paraffin sections at routine neuropathological examination. In this short report, we provide two staining protocols for visualizing cortical 100-300 sections. For low-power microscopy, first protocol combines aldehyde fuchsine detection lipofuscin granules macrophages...
Abstract The entorhinal cortex (EC) is the main interface between sensory association areas of neocortex and hippocampus. It crucial for evaluation processing data long‐term memory consolidation shows damage in many brain diseases, example, neurodegenerative such as Alzheimer's disease developmental disorders. pre‐alpha layer EC humans (layer II) displays a remarkable distribution neurons islands. These cellular islands give rise to portion perforant path—the major reciprocal stream...
Neuropathologic studies of brains from autopsy series show tau inclusions (pretangles, neuropils threads, neurofibrillary tangles) are detectable more than a decade before amyloid-β (Aβ) deposition in Alzheimer's disease (AD) and develop characteristic manner that forms the basis for AD staging. An alternative position views pathological without Aβ as 'primary age-related tauopathy' (PART) rather prodromal AD. Recently, an early focus Ammon's horn second sector (CA2) with relative sparing...
Abstract Immunohistochemistry on archival human brains is often limited because several conditions arise that complicate the use for high-resolution fluorescence microscopy. In this study, we developed a novel clearing approach immunofluorescence-based analysis of perfusion- and immersion-fixed post mortem brain tissue, termed hCLARITY. hCLARITY optimized specificity by reducing off-target labeling yields very sensitive stainings in sections allowing super-resolution microscopy with...