A5056561031- Advanced MRI Techniques and Applications
- Advanced NMR Techniques and Applications
- Mitochondrial Function and Pathology
- Neonatal and fetal brain pathology
- Atomic and Subatomic Physics Research
- Cardiac Imaging and Diagnostics
- Cardiovascular Function and Risk Factors
- Cerebrospinal fluid and hydrocephalus
- Metabolism and Genetic Disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Quantum, superfluid, helium dynamics
- Elasticity and Material Modeling
- Neurogenesis and neuroplasticity mechanisms
- Optical Imaging and Spectroscopy Techniques
- Tuberous Sclerosis Complex Research
- Fetal and Pediatric Neurological Disorders
- Vascular Tumors and Angiosarcomas
- Bipolar Disorder and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Adaptive Filtering Techniques
- Medical Imaging Techniques and Applications
- Hearing Loss and Rehabilitation
- Neurofibromatosis and Schwannoma Cases
- Voice and Speech Disorders
- Sarcoma Diagnosis and Treatment
Cincinnati Children's Hospital Medical Center
2019-2025
University of Cincinnati
2012-2024
University of California, San Francisco
2023
National Cancer Institute
2023
Boehringer Ingelheim (Germany)
2023
Boehringer Ingelheim (Austria)
2023
University of Cincinnati Medical Center
2023
Sabin Vaccine Institute
2015
The brain’s neuroreparative capacity after injuries such as ischemic stroke is partly contained in the neurogenic niches, primarily subventricular zone (SVZ), which lies close contact with cerebrospinal fluid (CSF) produced by choroid plexus (ChP). Despite wide range of their proposed functions, ChP/CSF remain among most understudied compartments central nervous system (CNS). Here, we report a mouse genetic tool (the ROSA26iDTR line) for noninvasive, specific, and temporally controllable...
Two recently developed novel rodent models have been reported to ablate microglia, either by genetically targeting microglia (via Cx3cr1-creER: iDTR + Dtx) or through pharmacologically the CSF1R receptor with its inhibitor (PLX5622). Both widely used in recent years define essential functions of and led high impact studies that moved field forward.Using Cx3cr1-iDTR mice combination Dtx via PLX5622 diet we compared two MRI histology study general anatomy brain CSF/ventricular systems....
Abstract This work demonstrates the first whole brain “high spatial resolution” 7 Li MR spectroscopy imaging in bipolar disorder subjects. The vivo quantification is validated by a phantom containing 5 mM lithium salt using identical radiofrequency sequence and protocol. study demonstration of distribution patients on therapy 3D approach. results show that level strongly correlated with serum concentration. brain‐to‐serum ratios for average local maximum were 0.39 ± 0.08 ( r = 0.93) 0.92...
ABSTRACT The quantification of cardiac motion using magnetic resonance imaging (CMR) has shown promise as an early-stage marker for cardiovascular diseases. Despite the growing popularity CMR-based myocardial strain calculations, measures complete spatiotemporal strains (i.e., three-dimensional over cycle) remain elusive. Complete calculations are primarily hampered by poor spatial resolution, with rapid wall also challenging reproducibility such strains. We hypothesize that a...
Neonatal hydrocephalus affects about one child per 1000 births and is a major congenital brain abnormality. We previously discovered gene mutation within the coiled-coil domain-containing 39 (Ccdc39) gene, which causes progressive (prh) phenotype in mice due to lack of ependymal-cilia-mediated cerebrospinal fluid (CSF) flow. In this study, we used CRISPR/Cas9 introduce Ccdc39 into rats, are more suitable for imaging surgical experiments. The Ccdc39prh/prh mutants exhibited mild...
The creatine transporter (CrT) maintains brain (Cr) levels, but the effects of its deficiency on energetics adaptation under stress remain unclear. There are also no effective treatments for CrT deficiency, second most common cause X-linked intellectual disabilities. Herein, we examined consequences in and stress-adaptation responses plus intranasal Cr supplementation. We found that CrT-deficient (CrT-/y) mice harbored dendritic spine synaptic dysgenesis. Nurtured newborn CrT-/y maintained...
Abstract The brain’s neuroreparative capacity after injuries such as ischemic stroke is contained in the neurogenic niches, primarily subventricular zone (SVZ), which lies close contact with cerebrospinal fluid (CSF) produced by choroid plexus (ChP). Despite wide range of their proposed functions, ChP/CSF remain among most understudied compartments central nervous system (CNS). Here we report a mouse genetic tool (the ROSA26iDTR line) for non-invasive, specific, and temporally controllable...
Individuals with neurofibromatosis type 1 develop rat sarcoma virus (RAS)–mitogen-activated protein kinase–mitogen-activated and extracellular signal-regulated kinase (RAS-MAPK-MEK)–driven nerve tumors called neurofibromas. Although MEK inhibitors transiently reduce volumes of most plexiform neurofibromas in mouse models (NF1) patients, therapies that increase the efficacy are needed. BI-3406 is a small molecule prevents Son Sevenless (SOS)1 interaction Kirsten viral oncoprotein (KRAS)-GDP,...
Tuberous sclerosis complex (TSC) is characterized by multisystem, low-grade neoplasia involving the lung, kidneys, brain, and heart. Lymphangioleiomyomatosis (LAM) a progressive pulmonary disease affecting almost exclusively women. TSC LAM are both caused mutations in TSC1 TSC2 that result mTORC1 hyperactivation. Here, we report single-cell RNA sequencing of lungs identified activation genes sphingolipid biosynthesis pathway. Accordingly, expression acid ceramidase (ASAH1) dihydroceramide...
Neonatal hydrocephalus is a congenital abnormality resulting in an inflammatory response and microglial cell activation both clinically animal models. Previously, we reported mutation motile cilia gene, Ccdc39 that develops neonatal progressive (prh) with microglia. We discovered significantly increased amoeboid-shaped activated microglia periventricular white matter edema, reduced mature homeostatic grey matter, myelination the prh model. Recently, role of models adult brain disorders was...
Background An imaging method that allows quantitative fibrosis estimates is needed to facilitate the diagnosis of chronic liver disease. Amide proton transfer (APT) and tissue sodium concentration (TSC) could meet this need. Hypothesis APT TSC correlate with in a mouse model Study Type Prospective. Phantoms/Animal Model Male C57Bl/6 mice given CCl 4 or vehicle ( N = 8 each) twice weekly for 16 weeks. Field Strength/Sequence Liver T 1 (Look‐Locker gradient recalled echo [GRE] sequence), 2...
Fast apparent transverse relaxation (short T 2 *) is a common obstacle when attempting to perform quantitative 1 H MRI of the lungs. While * times are longer for pulmonary hyperpolarized (HP) gas functional imaging (in particular gaseous 129 Xe), can still lead inaccuracies sequences requiring echo (such as diffusion weighted images) or readout duration spiral sequences). This especially true in preclinical studies, where high magnetic fields shorter than typically seen human studies....
Introduction: Left ventricular diastolic dysfunction (LVDD) is often manifested by impaired relaxation and myocardial stiffening in the left ventricle (LV). While impairments function of myofilament regulatory proteins are known to deteriorate LV relaxation, effects passive on active forces remain unknown. Methods: A diabetic murine model LVDD (db/db, n=5) wild-type mice (WT, were used study association between tissue-level stiffness organ-level relaxation. Early transmitral flow velocity...
Purpose Diffusion and lung morphometry imaging using hyperpolarized gases are promising tools to quantify pulmonary microstructure noninvasively in humans animal models. These techniques assume the motion encoded is exclusively diffusive gas displacement, but impact of cardiac on measurements has never been explored. Furthermore, although diffusion validated against histology mice 3 He, it for 129 Xe. Here, we examine effect validate Xe mice. Theory Methods Mice were imaged...
The trachea expands and retracts almost uniformly while breathing, but these dynamics are altered by congenital malformation injury to tracheal cartilage. Tracheal collapse—tracheomalacia—is a common comorbidity in of disorders, including bronchopulmonary dysplasia (BPD), idiopathic pulmonary fibrosis (IPF) cystic (CF) can cause life-threatening airway obstruction. While tracheomalacia is clinically diagnosed via bronchoscopy, no tool exists noninvasively assess small animal models. Here we...
Hypoxia-ischemia (HI), which disrupts the oxygen supply-demand balance in brain by impairing blood supply and cerebral metabolic rate of (CMRO 2 ), is a leading cause neonatal injury. However, it unclear how post-HI hypothermia helps to restore balance, as cooling reduces CMRO . Also, transient HI leads secondary energy failure (SEF) brains remains elusive. Using photoacoustic microscopy, we examined effects on awake 10-day-old mice, supplemented bioenergetic analysis purified cortical...
Hypoxia-ischemia (HI), which disrupts the oxygen supply-demand balance in brain by impairing blood supply and cerebral metabolic rate of (CMRO 2 ), is a leading cause neonatal injury. However, it unclear how post-HI hypothermia helps to restore balance, as cooling reduces CMRO . Also, transient HI leads secondary energy failure (SEF) brains remains elusive. Using photoacoustic microscopy, we examined effects on awake 10-day-old mice, supplemented bioenergetic analysis purified cortical...
Hyperpolarized (HP) xenon-129 (